Zhongshan Daxue xuebao. Yixue kexue ban, 1999 目的: 构建携带人神经生长因子( hNGF)基因的真核细胞表达载体, 为应用 NGF 进行老年性痴呆(AD)等疾病基 因治疗打基础。方法: 应用基因重组技术将 hNGF cDNA 克隆到逆转录病毒载体 pLXSN 中 ,用限制性内切酶酶切分析重组质粒 pLNGFSN 中 hNGF 基因的插入方向,用 PCR、斑点杂交和 Southern 杂交对重组质粒 pLNGFSN 作进一步鉴定。结果: hNGF cDNA 已正确地克隆到逆转录病毒载体 pLXSN 中,而构建成重组逆转录病毒载体 pLNGFSN ...
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[Overexpression of lncRNA SNHG12 promotes docetaxel resistance of prostate cancer cells by activating PI3K/AKT signaling <i>via</i> interacting with ELAVL1]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoZhao C +5 more
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[Myelodysplastic neoplasms with acute myeloid leukemia-like mutations: clinical features, molecular profiles, and prognosis]. [PDF]
Zhonghua Xue Ye Xue Za ZhiBao ZF +13 more
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[IgM- κ and IgM- λ biclonal POEMS syndrome combined with essential thrombocythemia: a case report]. [PDF]
Zhonghua Xue Ye Xue Za ZhiWang J, Wu F, Zhai ZM.
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Zhongshan Daxue xuebao. Yixue kexue ban, 1990 将α-心钠素（α-hANF）基因克隆到PUC18载体上,经菌落原位杂交筛选鉴定出有α-hANF基因的克隆株。用BamⅡ和BamH Ⅰ消化并分离纯化得到含5′端部分α-hANF基因及PUC18载体的F1片段。用DNA合成仪合成3′端部分α-hANF基因的F2接头,在基因3′端加入谷氨酸的密码子GAA,作为表达产物用内肽酶Glu-C专一裂解的位置,并去掉基因3′端的终止信号TGA,将原BamH Ⅰ接头改为EcoR Ⅰ接头。最后,将F1和F2片段与α-hANF基因酶促连接后进行转化 ...
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[Antitumor component-Ι in <i>Agkistrodon halys</i> venom inhibits proliferation and migration of cisplatin-resistant gastric cancer cells by downregulating RAI14]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLi Y +7 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2013 【目的】 分析预测申克孢子丝菌ｃＤＮＡ中编号为Ｌｏｃｕｓ＿１６８＿Ｃｏｎｔｉｇ＿１序列编码的基因及蛋白质结构、功能特征，并进行分子克隆。 【方法】 利用ＮＣＢＩ及Ｅｘｐａｓｙ网站提供的ＢＬＡＳＴｘ、ＳｉｇｎａｌＩＰ、ＩｎｔｅｒＰｒｏ Ｓｃａｎ等生物信息学软件分析其序列，判断其是否为全长编码序列、同源序列名称，并分析其结构、定位及功能等；将其中的全长编码区利用ＰＣＲ方法进行扩增后克隆到ｐＥＴ－３０ａ（＋）载体，测序验证。 【结果】 该序列是亚精胺合成酶（ＳＰＤＳ）的同源基因，全长序列为１０６２ ｂｐ ...
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[<i>Qingjie Fuzheng</i> Granules alleviates 5-fluorouracil-induced skeletal muscle injury in tumor-bearing mice by inhibiting mitochondria-dependent apoptosis and activating the AMPK-PGC-1α pathway]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoZhao J +5 more
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[Clinical and molecular characteristics of myeloproliferative neoplasms patients with NFE2 gene mutations]. [PDF]
Zhonghua Xue Ye Xue Za ZhiZhao SY +15 more
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[Proteomics analysis of the effect and mechanism of ADSCs on full-thickness skin defects in diabetic rats]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiGu Y, Chen YZ, Wang SY, Song HY, Bai N.
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