Results 111 to 120 of about 31,733 (245)

产超广谱β-内酰胺酶表型及基因型特征的研究

open access: yesZhongguo shiyan zhenduanxue, 2011
目的分析大肠埃希菌、肺炎克雷伯菌临床分离株中超广谱β内酰胺酶(ESBL)的基因型分布情况。方法用表型确证试验确定临床标本中产ESBL的大肠埃希菌和肺炎克雷伯菌分别用TEM、SHV和CTX-M通用引物进行聚合酶链反应(PCR)扩增,鉴定其基因型。结果 66%的菌株为TEM基因型,14%的菌株为SHV基因型,10%的菌株为CTX-M型。结论大肠埃希菌、肺炎克雷伯菌临床分离株中超广谱β内酰胺酶(ESBL)的基因型分布以TEM基因型为主,部分菌株为SHV、CTX-M型。同一菌株内可以产生2种不同基因型的超广谱β-
黄晶, 许建成, 安倍莹, 白岩
doaj  

Screening of SNP Loci of Growth-related Candidate Gene and Genetic Diversity of Five Populations Based On Microsatellite Markers in Fujian Oyster, Crassostrea angulata [PDF]

open access: yes, 2015
福建牡蛎(Crassostreaangulata,C.angulata)又名葡萄牙牡蛎,具有重要的经济价值和生态价值,但由于长期未经选育以及人工育苗的应用,导致牡蛎养殖出现经济性状衰退及死亡率逐年增高等现象,亟需加强种质资源保护,开展遗传改良研究。本研究利用单核苷酸多态性分子标记(singlenucleotidepolymorphism,SNP)对福建牡蛎生长相关的5个候选基因,即胰岛素相关多肽(insulinrelatedpeptides,IRPs)、ɑ-淀粉酶(ɑ-Amylase,ɑ-AMY ...
楚瑞敏
core  

Learning Styles, Engagement and Anxiety in AI‐Mediated Writing: A Multimodal Feedback Study

open access: yesInternational Journal of Applied Linguistics, EarlyView.
ABSTRACT Artificial intelligence (AI) tools now permeate English academic writing. However, evidence on how feedback modalities align with student differences and with psychological mechanisms remains limited. Prior work often reduced learning styles to simple matches with delivery modes and treated learning engagement and writing anxiety as peripheral.
Yi Ren   +3 more
wiley   +1 more source

Design, synthesis, and biological evaluation of AHPN derivatives as RARγ agonists [PDF]

open access: yes, 2016
RARγ是核受体超家族的重要成员,可以参与调控细胞生长、分化、凋亡、代谢和胚胎发育等许多重要生理过程。RARγ表达水平和功能的改变将可能导致肿瘤的发生,因而被认为是肿瘤治疗中的一个重要靶点。 6-{3'-(1-金刚烷基)-4'-羟基苯基}-2-萘甲酸(AHPN)是已经报道的选择性RARγ激动剂,可以通过调控RARγ的功能从而发挥诱导细胞凋亡的作用,对急性和慢性淋巴细胞白血病有潜在的治疗作用,然而在使用的过程中出现了视黄酸类化合物常见的不良反应,限制了其成药。 本课题以AHPN母环为结构基础,以RARγ ...
沈宇飞
core  

Synthesizing Empirical Research on Learner Metacognition in Second Language Writing

open access: yesInternational Journal of Applied Linguistics, EarlyView.
ABSTRACT Despite considerable research interest in learner metacognition in second language (L2) writing, there is not yet a review that synthesizes the existing empirical findings and discusses their implications. To fill the void, this systematic review synthesized 39 empirical studies on learner metacognition in L2 writing and examined their salient
Chen Li, Icy Lee, Zhicheng Mao
wiley   +1 more source

PCR-SSP对潜在输血需要住院患者ABO基因分型的检测效果与意义

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2013
【目的】 调查潜在输血需要住院患者的ABO血型基因,分析ABO表型相同输血中ABO基因型的错配输血几率。【方法】 对502例潜在输血需要的住院患者,采用血清学方法和聚合酶链反应-序列特异性扩增技术(PCR-SSP)检测ABO血型的表型及基因型。比较两种方法学对ABO血型表型的一致率;统计各ABO等位基因及各ABO基因型的频率;并分析潜在输血需要的住院患者在接受ABO血型表型相同的输血时,ABO基因错配输血几率。 【结果】 血清学与PCR ...
doaj  

白血病基因诊断及其信息系统建立

open access: yesZhongguo shiyan zhenduanxue, 2006
目的用基因诊断技术对白血病分型诊断及微小残留病(MRD)监控,同时对所检测数据建立信息系统。方法巢式RT-PCR扩增急髓性白血病融合基因,PCR扩增急性淋巴细胞白血病基因重排,用Delphi程序设计信息系统。结果M2b型白血病能扩增出AML1/ETO融合基因;M3型白血病能检测出L型和S型融合基因;慢粒白血病可检测出b3a2和b2a3融合基因;急淋白血病可检测出IgH、TCRγ、TCRδ基因重排。结论基因诊断能有效进行白血病分型及MRD监控,信息系统的建立方便了检测信息的管理及查询。
孙新六
doaj  

MNK, IRR and FGFR genes in Haliotis diversicolor: molecular cloning, expression analysis and identification of SNPs associated with growth traits [PDF]

open access: yes, 2015
杂色鲍(Haliotisdiversicolor)是我国南方重要的养殖鲍种,研究与杂色鲍生长相关的基因,分析基因的表达规律,并筛查基因中与生长性状相关联的SNP位点,对于深入了解生长性状调控的分子机制,培育具有优良性状的新品种具有重要意义。本研究将MAPK互作激酶(MAPkinase-interactingkinase,MNK)、胰岛素相关多肽受体(Insulin-relatedpeptidereceptor,IRR)和成纤维细胞生长因子受体(Fibroblastgrowthfactorreceptor,
何佩茹
core  

From Presence to Policy: The Conditional Impact of Women's Representation in Top Management Teams in Chinese Local Governments

open access: yesPublic Administration, EarlyView.
ABSTRACT While public administration research has begun exploring the impact of women's representation in top leadership positions in public organizations, the conditions under which women's representation in top management teams (TMTs) produces policies specifically benefiting women remain underexamined.
Ting Huang   +3 more
wiley   +1 more source

中国东北汉族人群解偶联蛋白3基因-55C/T多态性与2型糖尿病相关性研究

open access: yesZhongguo shiyan zhenduanxue, 2010
目的探讨解偶联蛋白3(uncoupling protein 3,UCP3)基因-55C→T变异与中国东北地区2型糖尿病的关系。方法用聚合酶链反应-限制性片段长度多态性检测100例2型糖尿病患者(男/女为58/42)及113名糖耐量正常者(男/女为56/57)UCP3基因-55C→T变异的基因型。结果 2型糖尿病组与正常对照组三种基因型频率及等位基因频率分布差异均有显著性意义,P值分别为0.027和0.003,两组间携带T的基因型(CT+TT)频率差异有显著意义(P=0.008 ...
刘佳南   +4 more
doaj  

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