Results 21 to 30 of about 16,526 (215)
Big Cat Mortality in Subsistence Hunts in Amazonia 亚马孙雨林地区生计狩猎中的大型猫科动物致死情况研究
Wildlife Letters, Volume 3, Issue 1, Page 16-21, March 2025.We use data from a participatory biodiversity monitoring program to estimate the annual mortality of jaguars and pumas resulting from subsistence hunts in two Amazonian Extractive Reserves. We estimate that around 50 big cats are killed annually in the study area, which is consistent with an independent estimate for the same area and within the same ...
Elildo A. R. Carvalho Jr. +3 more
wiley +1 more source
中国南方一罕见迟发性脊椎骨骺发育不良大家系的表型和基因型的相关性研究
Zhongshan Daxue xuebao. Yixue kexue ban, 2022 目的揭示一罕见遗传性矮小症家系的分子发病机制，阐明患者表型和基因型的相关性，为今后的产前、植入前基因诊断奠定基础。方法在临床初诊、系谱分析的基础上，首先采用高通量测序技术对先证者及其家庭核心成员的DNA样品进行全外显子组测序。通过生信分析找出候选基因，在确定候选基因及其新变异后，采用PCR、Sanger测序、RT-PCR、q-PCR等方法对该新变异的致病性进行系列鉴定。结果通过全外测序、Sanger测序验证和生物信息分析，确定TRAPPC2基因及其携带的 c.94 del ...
黄颖 +4 more
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Constructhion and Eukaryotic Expression of FBXW7α and Effects On Migrationof Cholangiocarcinoma Cell [PDF]
, 2015 胆管癌是起源于胆管上皮的一种恶性肿瘤，近年来在全球的发病率逐年升 高，目前占据消化道肿瘤的3%,胆管癌患者5年生存率不足5%；并且这一状况 30年未曾改变。由于起病隐匿，发现比较困难，手术效果并不理想；为此我们 寄希望于采取新一代的基因诊断提高发现率；有人对人类多种肿瘤做了基因突变 测定，发现FBXW7在胆管癌中突变率较高。FBXW7有三种亚型，分别为FBXW7 α、FBXW7β和FBXW7γ；FBXW7α含有一个F-box基序，该基序直接与Skp 蛋白结合构成完整的SCF(Skp-cullin-F ...
翁中海
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Wildlife Letters, Volume 3, Issue 1, Page 22-29, March 2025.Human–wildlife conflict poses a major threat to conservation and livelihoods. Here, we outline two innovative approaches: one where local people monitor wildlife on their land using camera‐traps and receive tangible, significant benefits as a direct result of that recorded wildlife presence, and one where that approach is expanded into wider ...
Amy Dickman +8 more
wiley +1 more source
Analysis of Differentially Expressed Proteins in Self-Paired Sera of Advanced Non-small Cell Lung Cancer Patients Responsive to Gefin [PDF]
, 2009 Background and objective All the advanced NSCLC patients that received EGFR-TKI therapy will eventually relapse after a period of efficacy. The aim of this study is to investigate the serum biomarkers as potential predictive factors for the efficacy of ...
Ailin GUO +10 more
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A spatially explicit assessment of factors shaping attitudes toward African elephant conservation
Conservation Biology, Volume 39, Issue 1, February 2025.Abstract Conservation plans that explicitly account for the social landscape where people and wildlife co‐occur can yield more effective and equitable conservation practices and outcomes. Yet, social data remain underutilized, often because social data are treated as aspatial or are analyzed with approaches that do not quantify uncertainty or address ...
Sarah L. Carroll +9 more
wiley +1 more source
Exomic somatic variation of esophageal squamous cell carcinoma [PDF]
, 2015 目的：食管鳞状细胞癌（esophagealsquamouscellcarcinoma,ESCC）是全球常见的致死性恶性肿瘤。为进一步探讨其分子机制，我们利用全基因组外显子测序技术寻找食管鳞癌中多发的突变，包括点突变、结构变异等，并且进一步鉴定食管鳞癌中多发的单核苷酸变异（Singlenucleotidevariation,SNV）。在体细胞水平探讨食管鳞癌发生发展的分子机制。 方法：1.通过外显子测序生成并收集数据库ESCC测序数据。2.128对食管鳞癌外显子测序数据采用新一代基因序列比对方法 ...
李慧莉
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Wildlife Letters, Volume 2, Issue 4, Page 192-203, December 2024.Knowledge of the reproductive success of organisms in urban habitat fragments is needed to conserve biodiversity in our urbanizing world. We found that differences in nest success among birds in urban forest patches is explained by factors on three distinct spatial scales even across a limited urban development gradient.
Ohad J. Paris, Colin E. Studds
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2022 目的本研究通过第二代高通量测序（NGS）检测配对骨肉瘤转移灶和原发灶标本，分析骨肉瘤原发灶与转移灶的基因图谱差异，以期发现促进骨肉瘤发生和转移的相关分子及可能机制。方法12例转移患者的原发灶与转移病灶肿瘤组织样本，使用第二代高通量测序（NGS）进行检测，其中9对panel检测（678个基因）以及3对全外显子检测（WES），分析比较骨肉瘤原发灶与转移灶的基因图谱差异：基因拷贝数变异通过EXCAVATOR检测；DNA层面的融合突变通过Lumpy检测；RNA层面的融合突变通过Defuse + STAR ...
许明贤 +4 more
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