Results 51 to 60 of about 976,356 (101)
Zhongguo shiyan zhenduanxue, 2006 目的研究肝癌原癌基因c-MET突变和缺失。方法采用PCR-SSCP和DNA测序方法,对37例肝癌患者肿瘤组织标本的c-MET基因15-19外显子进行检测,研究c-MET基因的缺失和突变情况。结果发现5例有c-MET基因外显子15-19存在点突变,突变率为7.4%。结论c-MET基因的突变与肝癌的发生和发展有一定的联系。
王继生, 周斌, 徐鲁白, 郑志强
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SWEET1‐mediated glucose transport is crucial for energy availability in Arabidopsis
New Phytologist, Volume 249, Issue 4, Page 1816-1830, February 2026.Summary The stress‐responsive hormone abscisic acid (ABA) is known for its inhibitory effects on various physiological processes, including seed germination, often resulting in energy deprivation. Interestingly, ABA‐induced germination inhibition can be alleviated by exogenous glucose (Glc), mimicking a functional interplay between ABA and sugar ...
Xueyi Xue +3 more
wiley +1 more source
, 1999 目的: 寻找中国人肝豆状核变性病(WD)基因突变的形式及频率。方法: 应用 PCR-SSCP 法对 45名 WD 患者进行 基因 exon5、8、14、18筛选, 对确定异常者进行 DNA 序列分析。结果: 在 45 名患者 DNA 样本的 PCR 扩增产物中未发现 exon 18 泳动异常,而 3 名患者显示exon14 多态性泳动异常, 但这种泳动也可见于正常标本中。4例患者显示 exon 5 单链构象多态性, 序列分析表明为 T 插入突变,其突变频率为 8.
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Rare Metals, Volume 45, Issue 1, January 2026.ABSTRACT High‐entropy alloys (HEAs) have emerged as a transformative class of materials distinguished by their complex chemical compositions, unique microstructures, and remarkable mechanical and functional properties. Traditionally, the discovery and optimization of HEAs have relied on conventional methods, including trial‐and‐error experimentation ...
Chrispin Ouko Zamzu +2 more
wiley +1 more source
一种大麦Waxy基因SNP突变的PCR-CTPP标记引物及方法
, 2014 本发明公开了一种大麦Waxy基因SNP突变的PCR-CTPP标记引物，包括外引物G3935-TWF2和G3935-TWR2；内引物G3935-TNF2和G3935-TNR2；本发明还公开了一种大麦Waxy基因SNP突变的PCR-CTPP标记方法，包括以下步骤：采用CTAB法提取待检测植株基因组DNA；利用提取的DNA为模板进行特异性PCR扩增；将PCR扩增产物进行电泳，对基因型进行判定。本发明使用四个引物结合一次PCR和电泳，便能在大麦群体中快速且经济地筛选出携带G3935突变为T位点的糯大麦材料 ...
潘志芬 +4 more
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Integrative Conservation, Volume 4, Issue 4, Page 675-685, December 2025.We demonstrated that Anguilla japonica eDNA concentration was allometrically scaled with their body size in both the laboratory and riverine environments. Integrating body size allometry has the potential to improve population abundance estimates in the field, whereas further clarification is required for understanding eDNA production mechanisms ...
Toshiaki S. Jo +2 more
wiley +1 more source
, 2017 家族性渗出性玻璃体视网膜病变(familial exudative vitreoretinopathy,FEVR)是一种遗传性眼科疾病,主要特征为视网膜周边血管发育异常和病变。由于病变程度不同,临床表型可从无明显症状到眼睛完全失明。本文通过研究3个中国FEVR家系和1个散发型患者,探索其致病突变与疾病表型之间的关系。收集家系中患者及亲属的外周血制备基因组DNA,设计引物以扩增FEVR致病基因FZD4、LRP5、NDP和TSPAN12的外显子区域并通过Sanger法进行测序。结果发现 ...
杨业明 +6 more
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PCR-SSCP检测急性髓系白血病患者FLT3基因及FLT3/ITD基因突变
Zhongguo shiyan zhenduanxue, 2009 目的研究急性髓系白血病（AML）患者DNA水平FLT3基因及其内部串联重复（ITD）突变。方法采用多聚酶链反应（PCR）联合单链构象多态性（SSCP）方法检测43例不同免疫分型AML患者DNA水平FLT3基因及FLT3/ITD基因突变。结果43例AML患者经PCR扩增,琼脂糖凝胶电泳FLT3基因检测全部阳性,未发现FLT3/ITD基因突变;SSCP电泳FLT3基因检测全部阳性,43例AML患者中有15例（35%）出现FLT3/ITD基因突变。结论PCR-SSCP适用于检测急性髓系白血病FLT3 ...
黄晶 +4 more
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Risk, Hazards &Crisis in Public Policy, Volume 16, Issue 4, December 2025.ABSTRACT Background Hazardous levels of contaminants produced by anthropogenic activities are increasingly pervading residential environs and urban areas. Hazardous contamination can pose significant health risks for citizens dwelling in exposure pathways.
Rosina Johnson +3 more
wiley +1 more source
, 2003 用氧氟沙星(Ofl)和链霉素(Sm)分别处理纤细裸藻获得褪色突变株,电子显微镜观察显示细胞中存在残留质体,其中一个Ofl突变株质体有原初类囊体膜形成,而两个Sm突变株质体内有异常致密、发达的膜结构。用PCR方法检查了质体DNA的9个基因,显示所有突变株均有核糖体蛋白基因丢失,其中一个Sm突变株仍保留质体DNA的大部分基因,其余突变株质体DNA则基本丢失。通过差异显示和RT ...
王江新,施之新,徐旭东
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