外源性SHIP基因表达抑制生长因子介导的 K562细胞增殖及Akt磷酸化
【目的】 探讨SHIP基因对IL-3诱导K562细胞系增殖的抑制作用,阐明SHIP对K562细胞作用的机制65377;【方法】 将慢病毒质粒pReceiver-Lv31-FIV和pReceiver-Lv31-SHIP转染K562细胞;转染细胞与IL-3共培养,Western blot法检测K562细胞Akt磷酸化;观察转染野生型SHIP基因对K562细胞增殖的影响;末端脱氧核苷酸转移酶介导的dUTP缺失末端标记(TUNEL)法检测细胞凋亡65377;【结果】 重组慢病毒载体pReceiver-Lv31 ...
doaj
[Niemann-Pick disease with two missense mutations in SMPD1 gene: a case report and literature review]. [PDF]
Wei Y, Guo H, Xu JN, Chen YY, Shi HX.
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[<i>Huangqin Qingre Chubi</i> Capsule inhibits JAK/STAT-driven synovial angiogenesis in rheumatoid arthritis by suppressing the LncRNA EBLN3P/miR-369-3p/NFIX axis]. [PDF]
Sun M, Wang Y, Liu F.
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[Research on Bacteriophage Resistance: Coevolutionary Arms Race Between Bacteria and Phages Drives Novel Antibacterial Therapies]. [PDF]
Li R +7 more
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重组人高密度脂蛋白B族I型清道夫受体基因腺相关病毒血 清型2/1杂合载体的构建及其在HepG2肝细胞的表达
【目的】 构建含绿色荧光蛋白(EGFP)重组人高密度脂蛋白B族I型清道夫受体(SR-BI)基因腺相关病毒(rAAV)2/1杂合载体,观察其在HepG2肝细胞的表达65377;【方法】以pCMV.SPORT6-SR-BI质粒为模板PCR扩增目的基因cDNA,将其克隆至pSNAV2.0-IRES-EGFP-LacZa载体中,经酶切及测序鉴定后,脂质体介导转染BHK21细胞,经G418筛选得到含SR-BI基因的BHK/SR-BI-IRES-EGFP载体细胞株,用携带rep2-cap1基因的辅助病毒(rHsv ...
doaj
[miR-488-5p promotes osteogenic and neurogenic differentiation of rat bone marrow mesenchymal stem cells and enhances neuralized bone regeneration]. [PDF]
Zeng L +5 more
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[Expert consensus on perioperative management of tooth extraction in patients receiving denosumab therapy]. [PDF]
Ye L +27 more
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[Clinical analysis of 7 cases of childhood acute lymphoblastic leukemia with <i>PDGFRB</i> rearrangement]. [PDF]
Tao YQ +7 more
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[Clinical characteristics of DUX4-IGH fusion B-cell acute lymphoblastic leukemia in children]. [PDF]
Luo YX +4 more
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[Whole genome sequencing and analysis of familial nonsyndromic congenital tooth agenesis]. [PDF]
Zheng Y, Wang D, Jiang T, Yang D, Lu H.
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