Results 101 to 110 of about 14,849 (155)

Snail基因慢病毒载体的构建及其促进结肠癌细胞上皮间质化的研究

Zhongshan Daxue xuebao. Yixue kexue ban, 2012
【目的】 构建Snail基因慢病毒载体,研究Snail基因的表达与结肠癌细胞上皮间质化的关系。【方法】 利用Gateway Technology法构建Snail基因表达质粒和空白对照质粒,行慢病毒包装,成功构建Snail基因慢病毒载体后,在结肠癌细胞系caco2中转染Snail基因质粒及空白载体对照,构建稳转细胞株caco2- Snail和caco2-vc,并在LoVo细胞中瞬时转染上述质粒,对Snail及E-钙黏蛋白(E-cadherin)的表达进行检测,并用划痕 ...
doaj  

[Protective effect and mechanism of TSPAN9-mediated mitocytosis in interleukin-1β-induced rat chondrocyte senescence]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Chen Q, Da W, Luo N, Shen B.
europepmc   +1 more source

IRAK-4基因沉默对人成骨样细胞凋亡的影响

Zhongshan Daxue xuebao. Yixue kexue ban, 2013
【目的】研究siRNA沉默白细胞介素-1受体相关激酶-4(IRAK-4)基因对人成骨样细胞株MG63凋亡及凋亡相关基因Bcl-2、Bax表达的影响。【方法】 以Lipofectamine 2000为载体将IRAK-4-siRNA转染入MG63细胞(CON组:不加入任何转染试剂;SC组:以scrambled siRNA序列进行转染;KD组:以特异性IRAK-4-siRNA序列进行转染),应用流式细胞术及TUNEL法检测细胞凋亡水平,western blotting检测Bcl-2、Bax基因的表达。【结果】
doaj  

[Effect of Golgi membrane protein 1 on the proliferation, migration, and invasion of oral squamous cell carcinoma cells and its mechanism]. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Li S, Wen C, Yu L, Chen J, Feng H.
europepmc   +1 more source

PTEN基因对子宫内膜癌细胞PI3K/AKT通路的影响

Zhongshan Daxue xuebao. Yixue kexue ban, 2011
【目的】探讨PTEN基因在子宫内膜癌PI3K/AKT通路中的作用，评估PTEN基因在抗肿瘤治疗中的意义。【方法】利用慢病毒载体系统, 构建人PTEN基因RNA干扰慢病毒载体和PTEN基因过表达慢病毒载体,分别转染PTEN野生型的HEC-1A细胞和PTEN突变型的Ishikawa细胞, 建立PTEN 基因敲减及过表达的细胞模型，Western Blot方法检测PTEN基因敲减或过表达前后PTEN蛋白的表达和AKT通路的活化情况, MTT法检测慢病毒转染前后细胞增殖的变化 ...
doaj  

[Dihydroartemisinin inhibits the progression of oral squamous cell carcinoma]. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Shan T, Liu J, Liu T, Sun D, Wang X, Liu Y.   +5 more
europepmc   +1 more source

[Clinical and molecular characteristics of neonatal <i>Klebsiella</i><i>pneumoniae</i> sepsis]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Hebei Provincial Neonatal Sepsis Research Collaboration Group.
europepmc   +1 more source

[Role and mechanism of Wnt9a in human and mouse chronic wound healing]. [PDF]

Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Jia YH, Yuan YX, Hu DH, Guan H.
europepmc   +1 more source

[Novel clinical insights and frontier issues in alpha- fetoprotein-producing gastric cancer]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Luo B, Liu H, Xie W, Gong W.
europepmc   +1 more source

[Expression and significance of the <i>FABP6</i> long transcript in clear cell renal cell carcinoma]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Yin H, Wang Z, Shu F, Zhang Z, Liang H, Zhang S.   +5 more
europepmc   +1 more source