Results 91 to 100 of about 34,300 (180)

ABCA12 Frameshift Deletion in Domestic Cats With Ichthyosis Fetalis

open access: yesVeterinary Dermatology, EarlyView.
ABSTRACT Background Ichthyosis fetalis (IF), also known as harlequin ichthyosis, is a rare and often fatal autosomal recessive congenital skin disorder. It is characterized by thickened, hard skin plaques and deep skin fissures that limit mobility and cause malformations of the eyes, lips and ears.
Jeanna M. Blake   +2 more
wiley   +1 more source

无蹼壁虎线粒体全基因组及系统发育分析

open access: yes野生动物学报, 2017
经PCR扩增和序列测定,获得了无蹼壁虎(Gekko swinhonis)的线粒体基因组全序列,其总长为16 818 bp,含有13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个控制区。各基因的位置、排列顺序及转录方向均和大部分的有鳞目物种一致。无蹼壁虎线粒体基因组的碱基组成分别为:A(31.35%)、T(26.28%)、C(27.71%)、G(14.67%)。除Ser(GCU)缺失DHU臂外,其余的21个tRNA都可以形成典型三叶草结构。13个蛋白质编码基因中 ...
秦 峰 曾德龙 高成伟 秦新民
doaj  

Spatially Downscaling of Future Food Demand for Policy Planning

open access: yesWorld Food Policy, Volume 12, Issue 1, May 2026.
ABSTRACT Understanding the subnational dynamics of food demand, while accounting for the evolution of the agrifood system at the global level, remains a challenge for designing food policy to ensure food security. To link global agrifood systems with subnational food policy planning, we downscale per capita food demand for 62 food commodities at the ...
Chun Song   +3 more
wiley   +1 more source

携 HBx 基因逆转录病毒载体的构建

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2000
【目的】构建携 HBx 基因的逆转录病毒载体, 为研究 HBx 基因与肝癌生物学行为间的关系提供基础。【方法】采 用 PCR 技术从 HBV 全基因组中扩增 HBx 基因; 将 HBx 基因亚克隆至质粒 pLNSX, 构建质粒 pLNSHBx,磷酸钙-DNA 共沉淀 法将重组体导入包装细胞系 PA317, 检测培养上清病毒滴度。【结果】应用 PCR 技术成功地从 HBV 基因组中克隆出全长 HBx 基因, 并经序列分析证实; 建立了产病毒细胞株 PA317/HBx,检测其培养上清病毒滴度为 8.
闵 军, 刘彦文, 陈积圣
doaj  

Developing HMME‐Conjugated NIR‐II Activated Carbon Dots Nanospray for the Treatment of MRSA Biofilm Infections in Diabetic Wound via PTT/CDT/SDT

open access: yesRare Metals, Volume 45, Issue 4, April 2026.
ABSTRACT Diabetic wound infections are a common complication of diabetes, which severely impact patients' quality of life. Effective treatment of diabetic wound infections remains one major challenge in the clinic, partially due to the formation of bacterial biofilm and antibiotic resistance.
Liang Zhou   +8 more
wiley   +1 more source

Genomic responses to increased temperature and pollinator selection in Brassica rapa L.

open access: yesNew Phytologist, Volume 250, Issue 2, Page 1231-1247, April 2026.
Summary Rapid environmental change reshapes both abiotic stress and biotic interactions, yet it remains unclear how these combined forces structure plants' genomic adaptation. In particular, the joint influence of temperature and pollinator identity, two ecological axes undergoing simultaneous global shifts, has rarely been quantified at genomic ...
Yanqian Ding, Florian P. Schiestl
wiley   +1 more source

中国吉林省地区朝鲜族及汉族正常人群内皮型一氧化氮合酶基因4a/bVNTR多态性分布的比较研究

open access: yesZhongguo shiyan zhenduanxue, 2013
目的分析我国吉林省地区朝鲜族及汉族健康人群内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)基因4a/bVNTR多态性的分布。方法应用聚合酶链式反应(polymerase chain reaction,PCR)技术,检测183例吉林省正常人(其中朝鲜族人群92例,汉族人群91例)eNOS基因多态性,并结合统计学软件分析其分布特点。结果我国吉林省朝鲜族人群eNOS基因4a/bVNTR的aa、ab和bb基因频率分别为0.043 48、0.304 3和0.652
李淼   +5 more
doaj  

Street‐Level Institutional Work: How Social Workers Maintain, Disrupt, and Create the Rules of Social Organizations

open access: yesEuropean Policy Analysis, Volume 12, Issue 2, Spring 2026.
ABSTRACT Research has shown that street‐level workers may shape public policies, yet less is known about the processes through which they attempt to influence institutional arrangements. This gap is particularly evident in corporatist welfare states, where multiple organizations are involved in social policy implementation.
Olivia Mettang
wiley   +1 more source

雌激素受体基因多态性与绝经后女性代谢综合征的相关性研究

open access: yesZhongguo shiyan zhenduanxue, 2014
目的观察雌激素受体(ER)α和β基因多态与绝经后女性代谢综合征(MS)的关系。方法 311例无亲缘关系的绝经后MS患者和231例年龄相匹配的健康对照人群,采用聚合酶链反应-限制性片段长度多态性(PCRRFLP)技术,检测ERα(XbaⅠ、PvuⅡ)和β基因(RsaⅠ、AluⅠ)多态性,并采用SHEsis在线计算平台进行统计学分析。结果与对照组相比,MS患者体重、BMI、SBP、DBP以及血清FBS、GSP、TG水平显著高于对照组,而HDLC水平显著低于对照组(P0.05);对照组和MS组RR、Rr ...
孙树凯   +5 more
doaj  

Fear in Public Policy Compliance: Citizens' Response to Crisis‐Induced Policies

open access: yesEuropean Policy Analysis, Volume 12, Issue 2, Spring 2026.
ABSTRACT This article brings together social psychology and public policy literature in a mixed‐methods research design to explore how fear influences non‐compliance with challenging policies during crises. Building on Terror Management Theory, we argue that fear affects individual citizens' compliance tendencies.
Stella Ladi   +3 more
wiley   +1 more source

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