Results 81 to 90 of about 71,543 (200)
Zhongguo shiyan zhenduanxue, 2010 目的研究转化生长因子β1（TGF-β1）基因启动子区-800G/A和第1外显子+915G/C基因多态性各等位基因及基因型在食管癌患者中的分布频率,初步分析其基因型及血清水平与食管癌的相关性。方法采用序列特异性引物聚合酶链反应（PCR-SSP）和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术,检测118例食管癌患者和130例正常对照组TGF-β1的基因多态性,包括TGF-β1基因启动子-800G/A、第1外显子+915G/C位点,同时采用酶联免疫吸附试验（ELISA）检测血清TGF-β1水平 ...
唐任光 +5 more
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Analysis on amide synthase and methyltransferase in biosynthesis of streptovaricin from the strain NRRL 2494 [PDF]
, 2015 曲张链菌素（streptovaricin）是由壮观链霉菌（Streptomycesspectabilis）NRRL2494产生的安莎类抗生素，与抗结核药物利福霉素具有相似的化学结构骨架，具有抗菌及免疫抑制等多种生物活性。本论文在对NRRL2494菌株基因组测序的基础上，获得曲张链菌素生物合成基因簇，并通过构建稳定的遗传操作体系，研究其生物合成途径中重要基因的功能，为利用组合生物学手段获得成药性好的曲张链菌素类化合物奠定基础。 本论文首次分析了NRRL2494菌株的基因组全长17.5MB序列 ...
宋姣姣
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International Journal of Applied Linguistics, EarlyView.ABSTRACT While English‐medium instruction (EMI) and English for Academic Purposes (EAP) courses are designed to promote language and content learning, their prevailing English‐only approaches often undermine students’ learning motivation. Against this backdrop, translanguaging has gained recognition as an effective pedagogical practice to support ...
Yihai Wen, Fan Fang
wiley +1 more source
comparative analysis of Litopenaeus vannanmei by transcriptome sequencing and characterization of two immune-relevant genes [PDF]
, 2015 南美白对虾是目前对虾养殖的主要品种，具有巨大的经济价值。然而，病害的发生严重危害其养殖业的发展，研究对虾的先天免疫反应能够给对虾养殖中病害的防治提供重要的理论基础。 转录组测序能够分析某一生理条件下细胞内所有mRNA的表达水平，本研究通过二代测序技术首次比较分析了对WSSV（whitespotsyndromevirus）抗性差异的两种南美白对虾群体的转录组，它们的基因表达水平存在明显差异，具有750个明显差异表达的基因，普通对虾及抗病对虾群体在病毒感染前后分别有479和132个差异表达基因 ...
徐景祥
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International Journal of Applied Linguistics, EarlyView.ABSTRACT With the increasing number of Chinese international students in Australian universities, intercultural competence has become a critical factor influencing their interactions with Australian tutors in university classroom settings. Existing research often examines students’ intercultural competence from a single‐method or single‐perspective ...
Jiangyu Han, Linda Tsung
wiley +1 more source
Molecular cloning, expression of heat shock protein 60 (HSP60) gene in Marsupenaeus japonicus and the association of its polymorphisms with thermal tolerance [PDF]
, 2015 日本囊对虾(Marsupenaeusjaponicus)是我国重要经济养殖虾类，但其在南方地区存在着高温度夏死亡率较高的养殖难题。因此，急需了解日本囊对虾的耐热性状及耐温的分子机制，为此，我们开展了日本囊对虾MjHSP60基因的克隆、表达分析及其多态性与耐热性状相关性的研究，获得了如下主要结果： 1.MjHSP60基因克隆 在日本囊对虾MjHSP60cDNA序列基础上克隆到了该基因DNA全序列。日本囊对虾MjHSP60基因全长4055bp，结构与拟穴青蟹一致，均由8个外显子及7个内含子构成 ...
李丽君
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International Journal of Applied Linguistics, EarlyView.ABSTRACT Many existing rubrics for L2 argumentative writing prioritize form‐based linguistic accuracy and complexity while insufficiently addressing substantive argumentative rigor and effectiveness. This limitation is further complicated by cross‐cultural variation in rhetorical conventions and educational practices, which challenge the direct ...
Zihan Xu, Peiyun Liu
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2002 【目的】研究地理因素对水蛭素基因结构变异的影响。【方法】抽提广东菲牛蛭基因组DNA , 根据已发表的水蛭 素基因设计一对引物, 从基因组DNA 扩增出一特异性的大约700 bp 片段, 再回收、克隆和测序。【结果】广东菲牛蛭水蛭素 基因全长为642 bp, 基因有4 个外显子, 被三个内含子隔开。与马尼拉菲牛蛭水蛭素基因Hm1 、Hm2 的同源性分别为90 %与 和88.6 %。【结论】地理因素会影响菲牛蛭水蛭素基因结构的变异。
谭恩光, 刘秀平
doaj
Journal of Sociolinguistics, EarlyView.ABSTRACT This study adopts a transnational raciolinguistic perspective to examine how Chinese international students (CISs) navigate language, race, and identity across borders and contexts. Based on semistructured interviews with 14 CISs, the study highlights that pre‐migration socialization in China influences how CISs perceive and interpret their ...
Gengqi Xiao, Hailing Wang, Jing Yu
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2015 目的调查疾病易感性相关的髓过氧化酶（MPO）、人类8-羟基鸟嘌呤糖苷酶（hOGG1）、锰超氧化物歧化酶（MnSOD）基因多态性在韩国人群中的分布状况。方法采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）技术分别分析300名韩国健康男女大学生的MPO基因启动子区-463bp位点、hOGG1基因第7外显子第1245位碱基C/G、MnSOD基因1183位点T/C的单核苷酸多态性—M-4,计算基因型和基因频率。结果 MPO基因型频率为G/G型80.3%、G/A型19.0%、A/A型0.7 ...
卢焕俊, 洪润哲, 许青松
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