Results 91 to 100 of about 36,876 (177)
【目的】 观察24个常用STR基因座等位基因突变的现象及特点65377; 【方法】 对5 084例肯定亲权关系的案例进行分析,筛选等位基因突变事件,确定突变等位基因的来源,统计各STR基因座的突变率,分析突变的规律及其特点65377; 【结果】 在24个STR基因座中共观察到172个突变事件,STR基因座的突变率介于0 ~ 0.492%65377;每个突变案例的突变基因座数目为1 ~ 3个65377;突变模式符合逐步突变模式,最多突变步数为四步65377;父系突变与母系突变比例为3.55:165377;
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青岛地区大肠埃希菌临床株中blaCTX-M和PMQR基因的检测
目的调查青岛地区大肠埃希菌临床分离株中β-内酰胺酶基因blaCTX-M和质粒介导的喹诺酮耐药(Plasmid-mediated quinolone resistance,PMQR)基因的流行性。方法采用Vitek-2compact系统进行菌株鉴定和药物敏感性测定。聚合酶链反应(Polymerase chain reaction,PCR)法检测菌株中存在的blaCTX-M基因和各种PMQR基因,并对PCR扩增产物进行DNA序列测定,确定基因类型。结果 83%的大肠埃希菌对头孢菌素类和氟喹诺酮类药物耐药 ...
尉鸿飞 +5 more
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Magnetization Dynamics Control for Noise Reduction in Fluxgate Sensors
ABSTRACT Fluxgate sensors are indispensable for the detection of weak magnetic fields. However, their noise performance remains constrained by an incomplete understanding of the interplay between the high‐frequency magnetization dynamics of the core material and the overall sensor behavior.
Chunyang Mu +11 more
wiley +1 more source
【目的】探讨中国人肝豆状核变性(WD)的分子发病机制和基因诊断的方法。【方法】应用基因工程技术对WD 进行了10 年的分子生物学研究。【结果】①WD 的基因定位研究:通过RFLP 及微卫星多态性分析, 应用两位点及多位点连 锁软件, 建立了中国人WD 基因在D13q14 .2 -3区域的精细遗传连锁图谱, 从而首次对中国人WD 基因进行了精确定位;②WD 基因突变研究:应用PCR-SSCP 及DNA 测序技术, 对39 个家系45 名WD 患者进行该致病基因的21 个外显子突变筛选, 发现 WD
徐评议 +4 more
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Flexible, Robust Transparent Conductive Films With Excellent Electromagnetic Shielding Properties
ABSTRACT Transparent conductive films (TCFs) are indispensable components for next‐generation wearable electronics, intelligent robots, and electromagnetic interference (EMI) shielding devices. However, the fabrication of conventional random metallic mesh suffers from an inherent trade‐off between “randomness and controllability”.
Shanghao Lu +5 more
wiley +1 more source
OPN基因启动区-156delG多态性与中国HBV相关肝细胞癌的易感性
【目的】研究骨桥蛋白(OPN)基因启动区多态性与乙型肝炎病毒相关肝细胞癌(HBV-HCC)遗传易感性的关 系。【方法】225例确诊为HBV-HCC患者与200例年龄匹配的慢性乙型肝炎患者被纳入研究。OPN基因启动区3个多态性 位点(-156delG/G ,-443T/C和-616T/G)通过直接测序法确定基因型。【结果】HBV-HCC组-156 delG/delG基因型分布频率高 于对照组(P = 0.003)。HCC组-156 delG等位基因频率显著增高(P < 0.001 ...
时红, 吴元凯, 林国莉, 李向永
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ABSTRACT Lithium–sulfur batteries have attracted extensive interest owing to their exceptionally high theoretical energy density and environmental benignity. However, their practical performance remains severely constrained by intrinsic challenges, including the polysulfide shuttle effect and large volume expansion during cycling, which markedly ...
Long Jiang +8 more
wiley +1 more source
经PCR扩增和序列测定,获得了无蹼壁虎(Gekko swinhonis)的线粒体基因组全序列,其总长为16 818 bp,含有13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个控制区。各基因的位置、排列顺序及转录方向均和大部分的有鳞目物种一致。无蹼壁虎线粒体基因组的碱基组成分别为:A(31.35%)、T(26.28%)、C(27.71%)、G(14.67%)。除Ser(GCU)缺失DHU臂外,其余的21个tRNA都可以形成典型三叶草结构。13个蛋白质编码基因中 ...
秦 峰 曾德龙 高成伟 秦新民
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ABSTRACT Gradient structures (GS) introduced by plastic deformation have been shown to effectively enhance the surface properties of metallic materials. However, the GS fabricated by existing methods often suffers from limited hardening depth and limited control over the GS layer, which constrain their engineering applications.
Yansong Guo +5 more
wiley +1 more source
目的观察雌激素受体(ER)α和β基因多态与绝经后女性代谢综合征(MS)的关系。方法 311例无亲缘关系的绝经后MS患者和231例年龄相匹配的健康对照人群,采用聚合酶链反应-限制性片段长度多态性(PCRRFLP)技术,检测ERα(XbaⅠ、PvuⅡ)和β基因(RsaⅠ、AluⅠ)多态性,并采用SHEsis在线计算平台进行统计学分析。结果与对照组相比,MS患者体重、BMI、SBP、DBP以及血清FBS、GSP、TG水平显著高于对照组,而HDLC水平显著低于对照组(P0.05);对照组和MS组RR、Rr ...
孙树凯 +5 more
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