Results 21 to 30 of about 34,300 (180)

Elevation, soil pH and calcium availability shape regional and local scale spatial patterns of PhoD gene abundance in tropical and subtropical forests

open access: yesFunctional Ecology, EarlyView.
Read the free Plain Language Summary for this article on the Journal blog. Abstract Organic phosphorus mineralization is a critical process in the phosphorus cycle, governing phosphorus bioavailability for plants. The PhoD gene, which encodes the key enzyme alkaline phosphatase, serves as a valuable biomarker for this process.
Sandhya Mishra   +3 more
wiley   +1 more source

Nitrogen deposition does not exacerbate phosphorus limitation of rhizosphere microbes in subalpine forests

open access: yesFunctional Ecology, EarlyView.
Read the free Plain Language Summary for this article on the Journal blog. Abstract The stoichiometric imbalance caused by nitrogen (N) deposition typically exacerbates phosphorus (P) limitation in plants. However, it remains unclear whether this effect extends to soil microbes, particularly those in the rhizosphere.
Jipeng Wang   +5 more
wiley   +1 more source

Diagnosing scaling bottlenecks in 10 community conservation initiatives in southern and eastern Africa

open access: yesConservation Biology, EarlyView.
Scaling area‐based conservation, including initiatives led or comanaged by Indigenous Peoples and local communities, is a flagship goal of the Kunming‐Montreal Global Biodiversity Framework. Conservationists often aspire to scale initiatives, but this is rarely achieved in practice. Identifying and addressing factors that limit initiative adoption (i.e.
Thomas Pienkowski   +39 more
wiley   +1 more source

膀胱移行细胞癌组织基因的P14ARF、DAPK、RARβ甲基化变异分析

open access: yesZhongguo shiyan zhenduanxue, 2012
目的通过检测新鲜冰冻膀胱癌组织中P14ARF、DAPK、RARβ基因的启动子CpG岛基因的甲基化状态,探讨P14ARF、DAPK、RARβ在膀胱癌发生过程中的作用及诊断意义。方法采用甲基化特异性PCR技术检测48例手术切除的膀胱癌组织和其中13例相应癌旁组织标本、17例非肿瘤患者正常膀胱组织中DAPK、RARβ、P14ARF基因的甲基化情况。结果 48例膀胱癌组织中发现P14ARF基因异常甲基化13例(27.0%),DAPK基因异常甲基化13例(27.0%);RARβ基因异常甲基化44例(91.6 ...
王凯臣   +5 more
doaj  

Representativeness of the Natura 2000 network for preserving plant biodiversity in the European Union

open access: yesConservation Biology, EarlyView.
Abstract The Natura 2000 (N2K) network of protected areas is one of the main tools for area‐based conservation in the European Union (EU), yet its role in preserving plant biodiversity requires better understanding. We examined data kept in the European Vegetation Archive from over 1.2 million vegetation plots and obtained over 14.2 million plant ...
Michele Di Musciano   +41 more
wiley   +1 more source

Use of species’ responses to cryptic anthropogenic disturbances for monitoring biodiversity outcomes in tropical forests

open access: yesConservation Biology, EarlyView.
Abstract Measuring area‐based conservation outcomes in tropical forests is challenging due to cryptic human disturbances (e.g., hunting). As a result, comparative studies of management strategies providing quantitative outcomes remain scarce, especially in the Neotropics.
Lucy Perera‐Romero   +5 more
wiley   +1 more source

雌激素受体α基因多态性与女孩性早熟关系的研究

open access: yesZhongguo shiyan zhenduanxue, 2010
目的探讨雌激素受体-α(ERα)基因多态性与江西地区女孩性早熟的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术,检测90例江西地区性早熟女孩和70例江西地区健康体检女孩ERα基因内含子1 Pvu Ⅱ T/C和XbaI A/G酶切位点基因多态性,并观察基因型对血清E2的影响。结果病例组ERα基因XbaⅠ基因型和等位基因频率分布与对照组相比差异有显著性 ...
柯江维   +6 more
doaj  

Using miniaturized laboratory equipment and DNA barcoding to improve conservation genetics training and identify illegally traded species

open access: yesConservation Biology, EarlyView.
Abstract Illegal wildlife trade (IWT) is one of the largest global illegal activities, and it negatively affects biodiversity and sustainable development worldwide. DNA barcoding coupled with high‐throughput sequencing (i.e., metabarcoding) is useful in identifying taxa affected by IWT and has been used routinely for decades.
Maria Joana Ferreira da Silva   +9 more
wiley   +1 more source

基因芯片技术在乙肝病毒基因型及YMDD变异检测中的应用

open access: yesZhongguo shiyan zhenduanxue, 2007
目的评价基因芯片技术在HBV基因型及HBVP基因YMDD变异检测中的应用价值,并对不同基因型病例的临床情况进行分析。方法抽提HBV DNA,用PCR方法扩增S基因及P基因部分区域,扩增产物与基因芯片上的寡核苷酸探针进行杂交,杂交结果通过芯片影像读取仪扫描到计算机上,经软件分析可得到HBV基因型、HBVYMDD野生型及YVDD、YIDD变异型结果;部分标本通过HBV preS/S基因序列测序证实。结果38份高病毒滴度标本(HBV DNA定量>1.0×105拷贝/毫升)中22例为B基因型(58 ...
陈伟烈   +5 more
doaj  

Success of restoration strategies in preventing extirpation of 2 critically endangered coral species

open access: yesConservation Biology, EarlyView.
Abstract An unprecedented marine heatwave in 2023 caused widespread coral bleaching and mortality throughout the Caribbean. In the Florida Keys (USA), 2 foundation species, elkhorn coral (Acropora palmata) and staghorn coral (Acropora cervicornis), were severely affected.
Erinn M. Muller   +29 more
wiley   +1 more source

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