Results 41 to 50 of about 10,299 (119)
Rare Metals, Volume 45, Issue 4, April 2026.ABSTRACT Constructing a porous scaffold with the essential features of natural cancellous bone, including structural architecture and biochemical cues, is one of the pivotal factors for effective bone regeneration. In this study, a biomimetic composite porous Gelatin fibrous scaffold (SrHA/Gelatin) incorporating ultralong strontium‐doped hydroxyapatite
Yin‐Chuan Wang +8 more
wiley +1 more source
In vitro culture, cryopreservation, and field reintroduction of the endangered Mingan thistle
Conservation Biology, Volume 40, Issue 2, April 2026.Abstract Current plant conservation efforts are hindered by, for example, poor seed germination, low viability, and insufficient propagation and preservation technologies. To address these problems, we devised an approach to plant conservation that integrates conservation, preservation, and restoration (CPR), which uses advanced in vitro techniques. We
Mukund R. Shukla +4 more
wiley +1 more source
TLR-2信号途径对新生儿免疫系统在淋巴细胞增殖和调节性T细胞扩增方面的影响
Zhongguo shiyan zhenduanxue, 2006 目的探讨TLR-2（Toll like receptor-2）信号途径对新生儿免疫系统在淋巴细胞增殖和调节性T细胞（Treg）扩增方面的影响及其与成人免疫系统的差别。方法应用TLR-2的配体-肽聚糖（PPG）和有丝分裂原-植物凝血素（PHA）刺激胎儿脐带血单核淋巴细胞（CBMC）和成人外周血单核淋巴细胞（PBMC）,然后采用3H胸腺嘧啶掺入法测淋巴细胞增殖情况及流式细胞技术检测活化T细胞,Treg细胞扩增情况。结果PPG和PHA刺激后,CBMC淋巴细胞总体明显增殖（P0.05）。PPG和PHA刺激后 ...
刘晶, 赵洪艳
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World Medical &Health Policy, Volume 18, Issue 1, March 2026.ABSTRACT Against the backdrop of calling for nonfemale‐oriented promotion of vaccination against human papillomavirus (HPV), this study explored three potential names—“HPV Vaccine,” “Cervical Cancer Vaccine,” and “Genital Warts Vaccine”—to promote vaccination against HPV infection among heterosexual men.
Timothy K. F. Fung +2 more
wiley +1 more source
PI3K/AKT/FKHRL1信号通路对CXLC16诱导的平滑肌细胞增殖的影响
Zhongguo shiyan zhenduanxue, 2008 目的探讨PI3K/AKT/FKHRL1信号转导通路在CXCL16诱导的血管平滑肌增殖中的作用。方法运用细胞计数法及MTT法检测CXCL16对于平滑肌细胞增殖的影响;免疫组化法观察CXCL16对PI3K/AKT/FKHRL1信号转导通路的作用,同时观察PI3K/AKT抑制剂LY294002对CXCL16诱导的上述变化的影响。结果CXCL16可明显诱导平滑肌细胞增殖,作用高峰时间在第4天,并且可增加磷酸化AKT及磷酸化FKHRL1的表达。PI3K ...
周珊珊, 魏岩, 郑杨
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Zhongguo shiyan zhenduanxue, 2017 目的研究亚砷酸钠（NaAsO2）对小鼠乳腺上皮细胞（NMuMG）增殖的抑制作用机制,为地砷病区乳腺癌致病机制提供理论依据。方法以不同浓度的NaAsO2（0,5,10,20,30μM）处理的NMuMG,观察其形态的变化;经Cell Counting Kit-8（CCK-8）测定不同浓度NaAsO2对NMuMG增殖情况;用实时定量RT-PCR分析增殖基因c-myc mRNAs的表达水平。结果在一定浓度范围内,随亚砷酸钠浓度升高,其细胞间隙变宽、形状不规则,甚至凋亡。在一定浓度范围内 ...
张明明, 李婷, 郑春雨, 董雪
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Zhongshan Daxue xuebao. Yixue kexue ban, 2008 【目的】探讨EGFR靶向治疗药物Iressa与顺铂联用对舌鳞癌细胞增殖的抑制作用。【方法】采用MTT的方法检测Iressa与顺铂联用对舌鳞癌细胞系Tscca和Tca8113抑制作用的效率。【结果】Iressa抑制Tscca和Tca8113细胞增殖的IC50值分别为18.03 μmol/L和29.98 μmol/L(P=0.002);Iressa与顺铂联用加强了对舌鳞癌细胞系Tscca、Tca8113的增殖抑制,而且当Iressa提前24h或48h使用时,这种协同作用更加明显。【结论 ...
孙传政 +7 more
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Zhongguo shiyan zhenduanxue, 2010 目的探讨抑肽酶对实验性急性肝损伤小鼠肝细胞增殖能力的影响。方法利用实验鼠的四氯化碳（CCl4）急性肝损伤模型,同时给予抑肽酶观察其对急性肝损伤小鼠肝细胞增殖能力改变。结果各治疗组与模型组相比,阳性细胞数目均显著增加 ...
王心童 +5 more
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Zhongguo shiyan zhenduanxue, 2009 目的研究辛伐他汀和胰岛素样生长因子-Ⅰ（IGF-Ⅰ）共同对人肺成纤维细胞（HFL-1）增殖的影响。方法培养人肺成纤维细胞,采用MTT法检测在辛伐他汀和IGF-Ⅰ单独/共同作用下对人肺成纤维细胞增殖的影响。结果辛伐他汀和IGF-Ⅰ共同作用于人肺成纤维细胞时,IGF-Ⅰ的促细胞增殖作用受到抑制。结论辛伐他汀可抑制IGF-Ⅰ对人肺成纤维细胞的促增殖作用。
孙文铸, 暴淑英
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Zhongguo shiyan zhenduanxue, 2010 目的探讨茶多酚对前列腺癌PC-3M细胞增殖及凋亡的影响。方法设立对照组与实验组,采用MTT比色法检测TP对PC-3M细胞增殖的影响;吖啶橙/溴化乙锭染色法观察诱导细胞凋亡情况;流式细胞术分析细胞周期;RT-PCR和Western blot检测Caspase-3基因的转录与表达。结果与对照组比较,MTT法示实验组对PC-3M细胞的增殖抑制作用增强;细胞染色法示实验组凋亡率增高;流式细胞术显示细胞阻滞于G1期,增殖指数降低;RT-PCR与Western blot示Caspase-3基因转录与翻译上调 ...
毛小强 +6 more
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