circATRNL1通过miR-21激活Smad4并抑制肺癌细胞的血管生成和转移标志物的表达
目的研究环状RNA趋化样1(circATRNL1)在非小细胞肺癌(NSCLC)中的机制,特别是其通过微小RNA(miR)-21调控Smad4信号及血管生成和转移标志物表达的影响。方法利用qRT-PCR检测circATRNL1和miR-21在正常肺上皮细胞和NSCLC细胞中的表达。通过双荧光素酶报告基因实验分析circATRNL1与miR-21的结合。对A549细胞进行circATRNL1过表达,并随后加入miR-21-mimic。通过Western blot法检测Smad4和p ...
张茜, 刘杰, 马红霞
doaj
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