Zhongguo shiyan zhenduanxue, 2007 目的本研究旨在探讨E1A基因对乳腺癌细胞凋亡的影响,为乳腺癌E1A基因治疗的可行性提供理论和实验依据。方法①构建E1A基因真核表达质粒pEGFP-E1A:通过酶切反应获得E1A基因,琼脂糖凝胶回收后,连接入pEGFP-C1载体EcoRⅠ和BamHⅠ位点中。②E1A基因转染乳腺癌细胞系:脂质体介导将E1A基因转入乳腺癌细胞系SK-BR-3细胞株,经筛选获得稳定表达E1A基因的转染试验组细胞。③RT-PCR检测E1A基因的mRNA表达、west-ern-blot检测E1A蛋白表达。流式细胞仪观察细胞凋亡率 ...
杨新宇 +3 more
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Zhongguo shiyan zhenduanxue, 2004 目的 检测p73、PTEN基因在大肠癌组织中的表达 ,并研究其临床病理意义。方法 采用逆转录聚合酶链式反应 （RT PCR）法检测p73、PTEN在 4 0例大肠癌中的表达。结果 ①DuckesA、B期大肠癌组织与对照组正常大肠黏膜组织p73、PTEN的表达均有非常显著性差异 （P
李有柱 +4 more
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[Overexpression of lncRNA SNHG12 promotes docetaxel resistance of prostate cancer cells by activating PI3K/AKT signaling <i>via</i> interacting with ELAVL1]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoZhao C +5 more
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[ERI3 expression is elevated in hepatocellular carcinoma and correlates with poor patient prognosis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoZhao X +8 more
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[PINK1 suppresses colorectal cancer cell growth through epigenetic regulation of histone modifications]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanWang M, Luan S, Fan X, Han D, Zhu Y.
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Advances of microRNAs Correlated with Invasion and Metastasis of Lung Cancer [PDF]
, 2010 Jiacong YOU, Qinghua ZHOU
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[Antitumor component-Ι in <i>Agkistrodon halys</i> venom inhibits proliferation and migration of cisplatin-resistant gastric cancer cells by downregulating RAI14]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLi Y +7 more
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[Effect of dephosphorylation of tumor metastasis suppressor gene <i>LASS2</i> on vacuolar ATPase activity and invasiveness of prostate cancer]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanLiu Y +8 more
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How epigenetics can explain human metastasis A new role for microRNAs [PDF]
, 2009 Amaia Lujambio, Manel Esteller
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[Fertility management in breast cancer patients: current strategies and research advances]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanZhou J, Jin M, Huang H.
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