Results 31 to 40 of about 2,333,295 (153)
目的为鉴定伤寒沙门菌的耐药性获得与整合子之间的关系,确定该菌抗性基因扩散的可能机制。方法我们选取32株多药耐药的伤寒沙门菌株提取全基因组DNA,扩增整合子,并以此作为模板检测抗性基因。结果以伤寒沙门菌基因组DNA为模板有81.2%的菌株,能够扩增出相应整合子的目的片段。具有四环素抗性、氨苄青霉素抗性和复方磺胺抗性的样品,以其扩增产物作为模板全部能扩增出相应抗性基因suⅡ、blaTEM-1和tetG。结论伤寒沙门菌的抗性基因是整合在Ⅰ类整合子中的,故整合子的转移应是伤寒沙门菌抗性基因传递的一种主要途径。
黄海, 李军, 黄健, 陶建蜀
doaj
ABSTRACT Photocatalytic H2 evolution coupled with biomass upgrading offers a sustainable route for clean energy conversion and high‐value chemical synthesis. Herein, an in situ grown S‐scheme MnS/MnB‐MBene heterojunction (denoted as SMx) was fabricated via alkaline etching of Mn2AlB2 followed by controlled sulfurization.
Pei Li +7 more
wiley +1 more source
目的研究临床分离铜绿假单胞菌的整合子Ⅰ和ISCR1的分布情况,并对其进行基因分型。方法分离临床234株铜绿假单胞菌,用WHONET5.4分析菌株药敏情况,PCR检测整合酶Ⅰ、整合子Ⅰ、ISCR1以及ISCR1携带的耐药基因。ERIC-PCR进行基因分型。结果铜绿假单胞菌对阿莫西林/克拉维酸、氨苄西林、氯霉素、头孢唑啉、米诺环素、氨苄西林/舒巴坦高度耐药,对环丙沙星、头孢他啶、头孢哌酮/舒巴坦、阿米卡星、亚胺培南、美洛培南较敏感,118株整合酶Ⅰ阳性,95株Ⅰ类整合子可变区阳性 ...
孙静静, 吴奎海, 芮勇宇
doaj
ABSTRACT Rationally selection of precursor additives during perovskite crystallization is essential for obtaining high‐quality films and achieving high‐performance perovskite solar cells (PSCs). However, the discovery and optimization of effective additives still rely heavily on time‐consuming and costly trial‐and‐error experiments.
Zhimin Feng +12 more
wiley +1 more source
ABSTRACT Esophageal squamous cell carcinoma (ESCC) remains associated with frequent local recurrence and distant metastasis despite advances in radiochemotherapy and immunotherapy, highlighting the need for therapeutic strategies that improve both local tumor control and systemic antitumor immune responses.
Dongdong Wang +7 more
wiley +1 more source
采用分步浸渍法制备了Ni-Mg整体式催化剂,加入萘作为焦油模型化合物考察了低温等离子体耦合催化重整生物质粗燃气的性能,研究了放电条件及反应温度对催化重整制备合成气的调变作用。结果表明:高压高频放电产生等离子体耦合催化重整,对重整净化生物质粗燃气有明显效果。在重整温度为750℃,放电频率为22kHz时CH4的转化率可由催化重整时的86%提高到94%左右,放电条件下可以将合成气的H2/CO比由1.06提高至1.15。温度对耦合重整影响较为显著,CH4、CO2的转化率均随温度的升高而升高,最高可分别达到94 ...
王铁军 +5 more
core
目的了解通辽地区大肠埃希菌临床分离株中I类整合子分布情况及基因盒类型,初步探讨耐药与整合子之间的关系。方法收集2013年10月-2014年3月通辽市某医院的非重复菌株作为试验菌株,采用Kirby-Bauer纸片法进行药敏试验;采用PCR方法扩增Ⅰ类整合酶基因,在此基础上扩增Ⅰ类整合子可变区,对部分基因盒阳性菌株测序并分析。结果 99株大肠埃希菌临床分离株对所试23种抗生素中的13种耐药率高于50%,主要为对青霉素、磺胺类药物、头孢菌素等;Ⅰ类整合酶检出55株,检出率为55.56%;基因盒携带有36株 ...
于新 +4 more
doaj
ABSTRACT Diabetic chronic wounds, characterized by a unique pathological microenvironment including persistent hyperglycemia, excessive reactive oxygen species (ROS), bacterial infection, and sustained inflammation, exhibit notoriously impaired and delayed healing processes. This study developed a multifunctional hydrogel therapeutic system (GOx@Zn‐MPN
Baowei Zhai +11 more
wiley +1 more source
信息流、物流、资金流三流的整合给企业带来低成本和高效率。物流是电子商务的基础,资金流是保证,信息流贯穿于物流和资金流的任何环节,指导着物流和资金流,三流的整合以信息流为主线。随着电子商务的发展,越来越多的电子企业认识到电子商务给企业带来的机遇,把对三流的整合作为其追求的目标 ...
罗贤春
core
Uncovering Cu Involved Nanophase Structures and Transformation in a Novel 7075B Aluminum Alloy
ABSTRACT In this study, aberration‐corrected transmission electron microscopy combined with first‐principles calculations was employed to systematically investigate the Cu‐involved crystal structures and evolution mechanisms of nanoprecipitates in a novel 7075B Al alloy. Firstly, an atomic model of the early‐stage GP‐ηp structure together with specific
Deyu Zhang +13 more
wiley +1 more source

