Results 31 to 40 of about 24,523 (167)
Zhongguo shiyan zhenduanxue, 2017 目的分析吉林省耐多药结核分枝杆菌临床分离株rpoB、katG基因突变特点,为建立本省快速耐多药检测方法提供参考。方法对吉林省耐多药结核分枝杆菌菌株扩增rpoB、katG基因测序后比对分析。结果耐多药结核分枝杆菌rpoB、katG基因的突变率分别为83.53%和70.6%,突变类型包括点突变、联合突变和缺失。在rpoB基因中,82.30%突变发生在RRDR区域,最常见突变位点为rpoB 531、rpoB 526、rpoB 516。KatG基因突变率为70.6%,最常见突变位点为katG315 ...
张炜煜 +4 more
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Physiological analysis on long shelf-life in ripening mutants (rin, nor, alc) tomato and their F1 [PDF]
, 1993 English abstract: Changes in respiration, ethylene production, PG activity and firmness during ripening period were monitored in fruits of the 'alcobaca', 'nor', 'rin' mutants and those of normal tomato (Lycopersicon esculentum Mill.) In fruits of ...
Lu, C, Xu, H, Zhao, Y
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Automating the analysis of public saliency and attitudes toward biodiversity from digital media
Conservation Biology, Volume 40, Issue 2, April 2026.Abstract Measuring public attitudes toward wildlife provides crucial insights into human relationships with nature and helps monitor progress toward Global Biodiversity Framework targets. Yet, conducting such assessments at a global scale presents challenges.
Noah Giebink +8 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2011 目的探讨K-ras基因突变谱与结直肠癌肝转移的相关性,为进一步阐明结直肠癌肝转移的分子机制提供理论依据。方法收集2008年1月至2010年8月在我院治疗的结直肠癌肝转移患者30例,进行K-ras基因突变检测,分析K-ras基因的突变类型及频率,探讨K-ras基因的突变谱与结直肠癌肝转移患者性别、年龄、原发灶位置、转移位置、病灶形态及TNM分期的相关性。结果 30例结直肠癌肝转移患者组织中K-ras基因突变者14例,突变率为47%,其中12密码子突变9例,突变率为30%;13密码子突变者5例,突变率为17%
王涛 +4 more
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Bayesian Analysis of Change-point Detection in Multivariate Time Series [PDF]
, 2016 时间序列结构突变检测问题是近年来的热点之一。Harléetal.(2014)提出了一种检验多元时间序列变点的贝叶斯方法。该方法用向量R刻画序列中每一点处变点发生的情况，通过曼-惠特尼检验计算序列每一点处的p值，并由这些p值构造替代的似然函数。最后，根据替代的似然函数以及吉布斯抽样得到对向量R的估计。该方法不要求变点个数事先给定，而是将其作为模型中的变量进行估计。 本文阐述了该方法的理论，并使用该方法对由人民币名义有效汇率同比增长率、美元名义有效汇率同比增长率 ...
秦超
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Elephant barrier behaviors in response to conflict mitigation fences
Conservation Biology, Volume 40, Issue 2, April 2026.Abstract Human–wildlife conflict is a major conservation issue, particularly in lower income countries, where it affects marginalized people and leads to the extirpation of threatened species. Managers increasingly use fences to reduce this conflict but lack evidence on the effectiveness of these barriers, especially on whether this reduces the number ...
Dominique Gonçalves +2 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2002 【目的】利用聚合酶链反应(PC R)技术对Wilson 病(WD)基因进行体外定点突变的研究。【方法】采用PCR 定点 突变技术, 首先设计两对引物, 将突变位点设计在引物上, 通过重叠延伸法两次PCR 扩增, 扩增片段上含有所需要的突变位 点, 最后将扩增片段克隆入pRc/ CMV 载体中。【结果】DNA 测序表明在预期位点已经发生突变,WD 基因第778 位密码子由 精氨酸(Arg)残基突变为亮氨酸残基(Leu), 用PCR 定点突变技术成功构建Wilso n 病基因突变体。【结论】PCR ...
闫振文 +4 more
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Zhongguo shiyan zhenduanxue, 2021 目的探讨肝硬化患者血浆游离DNA(cfDNA)中TERT基因-124(C/T)和-146(C/T)热点突变率及其突变与临床指标相关性。方法纳入航天中心医院2019年10月-2020年11月肝硬化患者80例,收集患者临床基本资料。EDTA抗凝全血分离500μL血浆提取cfDNA。TERT热点突变检测采用ARMS-qPCR方法。结果在80例肝硬化患者血浆cfDNA中,检测到TERT热点突变6例(7.5%),其中-124(C/T)4例(5%),-146(C/T)2例(2.5%)。与非突变组比较 ...
薛晓霞, 何美琳, 梁国威
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Impacts of Human Development on Wildlife Use of Corridors in Botswana 人类开发对博茨瓦纳野生动物廊道利用的影响
Integrative Conservation, Volume 5, Issue 1, Page 164-171, March 2026.This study examined the effect of landscape‐scale human impact on wildlife in Botswana. We examined how species use wildlife corridors in two different human‐dominated landscapes. We identified differences in temporal overlap between wildlife and humans across both landscapes, which is fundamental to consider in both current and future conservation ...
Tempe S. F. Adams +3 more
wiley +1 more source
Regulation of PI3K and MAPK inhibitors on the activity of FOXO1 and their effect on biological behavior of GIST-T1 gastric stromal tumor cell lines [PDF]
, 2015 胃肠道间质瘤（gastrointestinalstromaltumors,GISTs）是消化道最常见的间叶组织肿瘤。目前普遍认为GISTs起源于向cajal细胞分化的原始间叶细胞或幼稚干细胞，具有多向分化特征，可以向平滑肌、神经分化或不定向分化，是一种具有恶性潜能的胃肠道肿瘤，但其确切起源尚不明确。其发病率为1～2/10万人口,似乎正逐渐增加。近年来有研究表明，PI3K（磷脂酰肌醇-3-激酶）和MAPK（丝裂原活化蛋白激酶）信号通路活性在胃肠道间质瘤显著增高 ...
高华
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