Results 101 to 110 of about 6,835 (154)

转染survivin对HLF细胞增殖及凋亡的影响

Zhongshan Daxue xuebao. Yixue kexue ban, 2006
【【目的】 探讨survivin基因表达对人肺纤维母细胞（humanlungfibroblast，HLF）增殖、凋亡的影响。【方法】 构建survivin基因真核表达质粒peDNA3．1／SVN；利用脂质体转染法将其转入不表达survivin mRNA的HLF细胞系。连续培养10周。筛选出稳定表达的阳性克隆（HLF／SVN）及转空质粒对照（HLF／K）。用RT-PCR、免疫组化、Western blot检测细胞中survivin、bel-2和caspase3mRNA表达：通过观察细胞生长曲线 ...
顾霞, 林汉良, 朱有凯, 张萌, 梁惠珍, 沈艳   +5 more
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[A preliminary study on the role of platelet mitochondria in the proliferation and metabolism of multiple myeloma cells]. [PDF]

Zhonghua Xue Ye Xue Za Zhi
Zhang LY, Xiang YH, Li YY, Zhang J.
europepmc   +1 more source

[The role and mechanism of chemokine network in promoting osteoarthritis progression by regulating synovial macrophage heterogeneity]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Lin Y, Wu L, Chen Q, Shen B.
europepmc   +1 more source

[Shihu Mixture alleviates diabetic cardiomyopathy in rats by Sirt3-mediated upregulation of myocardial mitochondrial mitophagy pathway]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Lin X, He Y, Shi H, Liu J, Hu H.
europepmc   +1 more source

[Pathogenic mechanisms of Leber hereditary optic neuropathy caused by m.3472T>C mutation]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Zhang H, Shan W, Yasheng M, Guan M.
europepmc   +1 more source

[Knockdown of <i>Cav1</i> inhibits mitochondrial function and mRNA m⁶A modification and expression of key genes in mouse hepatocytes]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Ding S, Liao Y, Bai X, Huang J, Asakawa T.   +4 more
europepmc   +1 more source

[Isovitexin alleviates myocardial oxidative stress injury in diabetic mice by enhancing myocardial SIRT3 expression and reducing oxidative stress]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Peng Y, Jiang Y, Ma D, He A, Lü D, Luo M, Luo S.   +6 more
europepmc   +1 more source

[Resveratrol protects barrier function of mouse brain microvascular endothelial cell monolayers with oxygen/glucose deprivation and PM_2.5 exposure by maintaining mitochondrial dynamics balance]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Qin M, Sun S, Liu J, Gao Y, Wang H, Wang Y, Sun A, Yan J, Wang J, Yu Y.   +9 more
europepmc   +1 more source

人血管内皮生长因子165慢病毒载体的构建及其抗放射所致细胞凋亡的作用

Zhongshan Daxue xuebao. Yixue kexue ban, 2011
【目的】 构建人血管内皮生长因子165(hVEGF165)慢病毒表达载体pCDH-CMV-MCS-EF1-copGFPhVEGF165,并转染至HT22 细胞,并进一步观察其对放射线损伤所致的细胞凋亡的保护作用【方法】 PCR扩增hVEGF165基因,克隆到pCDH-CMV-MCS-EF1-copGFP载体;通过酶切电泳,菌落PCR初步筛选和测序鉴定构建的pCDH-CMV-MCS-EF-GFP1-hVEGF165重组载体;采用磷酸钙共转染法转染293T细胞包装制备慢病毒液,并感染HT22细胞 ...
doaj  

[<i>Qingjie Fuzheng</i> Granules alleviates 5-fluorouracil-induced skeletal muscle injury in tumor-bearing mice by inhibiting mitochondria-dependent apoptosis and activating the AMPK-PGC-1α pathway]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhao J, Peng J, Lin M, Zhu X, Huang B, Lin J.   +5 more
europepmc   +1 more source