Results 11 to 20 of about 726,547 (156)
雷帕霉素逆转卵巢癌细胞系SKOV3/DDP顺铂耐药及其机制探讨 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】研究雷帕霉素对卵巢癌顺铂耐药细胞系 SKOV3/DDP 的逆转作用并探讨其相关分子机制。 【方法】采用四甲基偶氮唑蓝（MTT）法测定卵巢癌顺铂耐药细胞系SKOV3/DDP的耐药倍数、雷帕霉素对其细胞毒 性及逆转倍数；Western blot法检测雷帕霉素对细胞内Akt/mTOR相关通路蛋白表达的影响。【结果】 ① MTT法检 测出雷帕霉素浓度分别为 25、50、100、500 和 1 000 μg/L 对卵巢癌顺铂耐药细胞系 SKOV3/DDP 的抑制率分别为 4.48%、25.30 ...
徐国才 +4 more
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野生动物学报为研究观赏禽类中分离的奇异变形杆菌（Proteus mirabilis）对第三代头孢菌素的耐药机制，于2019年从河南省某动物园禽类养殖区绿孔雀（Pavo muticus）、红绿金刚鹦鹉（Ara chloroptera）、火鸡（Meleagris gallopavo）、帽子鸡（polish chicken）和非洲鸵鸟（Struthio camelus）5种观赏禽类中分离17株奇异变形杆菌，经药物敏感性试验、blaCTX-M基因检测、全基因组测序、PFGE和RT ...
梁玉蕾 +5 more
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Journal of Diabetes, Volume 14, Issue 9, Page 606-619, September 2022., 2022 Highlights To what extent NAFLD is associated with glycemic remission among the general population remains unclear. The study elaborated that presence of NAFLD and fibrosis status decreased the chance of glycemic remission whereas NAFLD improvement mitigated glycemic deterioration, emphasizing joint management to liver‐focused subgroups of diabetes ...
Zhuojun Xin +15 more
wiley +1 more source
Journal of Diabetes, Volume 14, Issue 6, Page 401-413, June 2022., 2022 Highlights Insulin degludec/liraglutide (IDegLira) has good efficacy and tolerability in Chinese adults with type 2 diabetes not controlled on oral antidiabetic drugs. IDegLira provides the clinical advantages of insulin and glucagon‐like peptide 1 receptor agonists, improving glycemic control while reducing the main adverse effects of insulin (weight ...
Weiqing Wang +11 more
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Veterinary Dermatology, Volume 33, Issue 1, Page 17-e6, February 2022., 2022 Background – Because of the increased incidence of multidrug‐resistant (MDR) bacteria, the use of disinfectants over antibiotics has been encouraged. However, the interactions between disinfectants and host local immunity are poorly understood. Objective – To assess the effects of chlorhexidine digluconate (Chx), with and without selected host defence ...
Domenico Santoro +3 more
wiley +1 more source
Veterinary Dermatology, Volume 33, Issue 1, Page 55-e18, February 2022., 2022 Background – Canine atopic dermatitis (cAD) is a common chronic relapsing pruritic skin disease for which management commonly relies on life‐long use of immunomodulatory drugs. A number of the medications used are associated with adverse effects and the potential for complications during long‐term use.
Adrian Watson +3 more
wiley +1 more source
Veterinary Dermatology, Volume 32, Issue 6, Page 654-663, December 2021., 2021 Background Staphylococcus pseudintermedius is the main aetiological agent of canine pyoderma. Whole genome sequencing is the most comprehensive way of obtaining relevant genomic information about micro‐organisms. Hypothesis/Objectives Oxford Nanopore technology enables quality sequencing and de novo assembly of the whole genome of S.
Lluís Ferrer +13 more
wiley +1 more source
酪氨酸激酶抑制药GW2974诱导乳腺癌细胞BT474耐药的代谢机制研究 [PDF]
, 2019 目的通过比较酪氨酸激酶抑制药GW2974给药后母本细胞株BT474与耐药株r BT474之间代谢相关因子mRNA表达的差异,探讨r BT474可能的耐药机制。方法收集对数生长期r BT474和BT474细胞,接种于96孔板,加入含有GW2974的培养基,分别于孵育0,12,24和48 h后,通过噻唑蓝法检测BT474与r BT474细胞的增殖情况。用逆转录实时定量-聚合酶链反应检测12种相关代谢因子葡萄糖转运体4(GLUT4)、2,6-二磷酸果糖激酶(PFK-2)、丙酮酸激酶2(PKM2)、乳酸脱氢酶 ...
毛宇彬 +6 more
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Zhongguo shiyan zhenduanxue, 2021 目的构建骨肉瘤耐药细胞,探讨LIMK1(LIM Kinase1)逆转骨肉瘤耐药的作用及机制。方法通过长春新碱(VCR)诱导建立人成骨肉瘤MG63的耐药细胞亚系,应用半数抑制浓度公式计算IC50值及耐药指数评估细胞的耐药性;CCK-8法检测细胞增殖抑制率;相差倒置显微镜观察耐药细胞细胞形态的改变;PT-PCR实验检测耐药基因MDR1及其相关基因LIMK1的表达;转染pSUPER-LIMK1重组质粒,验证沉默LIMK1基因逆转MG63耐药的作用并分析其机制。结果本研究所构建的耐药细胞的耐药指数为7.01 ...
黄丽红 +4 more
doaj
, 2002 目前 ,抗菌药物在水产养殖动物疾病防治中占有重要地位 ,然而 ,在水产养殖中使用抗菌药物也可产生一系列的副作用 ,例如细菌耐药性的产生和药物在鱼体和环境中的残留。本文就水产药物使用的历史、现状、适应症、副作用 ,耐药性的现状、来源、产生机制 ,耐药菌判定的标准 ,以及该领域的研究现状等问题进行综述与分析 ...
李爱华
core +2 more sources