rAAV1-EGFP和rAAV2-EGFP转染犬骨髓间充质 干细胞的比较
【目的】 研究不同血清型腺相关病毒(rAAV)载体介导的增强绿色荧光蛋白基因(EGFP)对体外培养犬骨髓间充质干细胞(MSC)的转染效率及其毒性作用65377;【方法】 应用密度梯度离心法及贴壁筛选法分离培养犬MSC,倒置显微镜及Giemsa染色观察细胞形态,透射电镜观察其超微结构,流式细胞仪鉴定其表面标记物65377;取第3代MSC,以MOI为104 ~ 105 v.g./cell 的rAAV1-EGFP 和rAAV2-EGFP进行转染,3 d65380;7 d65380;21 ...
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[[abstract]]本发明关于一种重组腺病毒载体,可用于产生免疫力以对抗肠病毒感染。于一具体实施例中,本发明之重组腺病毒载体包含一表现卡匣,其编码肠病毒之P1蛋白及3CD蛋白酶。于另一具体实施例中,本发明重组腺病毒载体包含一表现卡匣,其编码肠病毒之3C蛋白酶或3CD蛋白酶。本发明亦关于一种疫苗组合物,包含所述之重组腺病毒载体。本发明提供一种使用该重组腺病毒载体及疫苗组合物以诱发个体产生免疫反应,对抗肠病毒感染的方法。进一步提供一种产生肠病毒之类病毒颗粒之方法 ...
周彦宏
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