Fe3O4@PDA标记的脐带间充质干细胞对高糖环境下肾小管上皮细胞自噬的影响
目的分析Fe3O4@PDA标记的脐带间充质干细胞(hUCMSCs) 在高糖(HG)环境下对肾小管上皮细胞(HK2细胞)的影响,探讨其对HG环境下HK2细胞自噬的作用机制。方法合成Fe3O4@PDA纳米粒子(Fe3O4@PDA NPs),培养hUCMSCs、HK2细胞,将HK2细胞分为4组,包括①对照组②HG组③Fe3O4@PDA hUCMSCs组④hUCMSCs组。通过CCK8检测各组HK2细胞活力,Western blot检测各组HK2细胞中自噬相关蛋白Beclin1和p62的表达水平,RT ...
张金妹 +4 more
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