急性白血病体外药物敏感性测定——一种简便、迅速、切实可行的方法 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 1985 用96孔培养板培养病人骨髓白血病细胞作药物抑制氚标记脱氧胸苷(~3H-TdR)参入试验。对14例白血病患者作26次试验,结果表明13例急性白血病24次试验药物在体外对~3H-TdR参入的抑制率与临床用药疗效相当一致,阿糖胞苷和三尖杉酯碱对~3H-TdR参入细胞的抑制试验可以有效地预测疗效,本法与其他试验方法比较,具有迅速简便,切实可行等优点。慢粒急变1例作2次试验,结果与临床疗效不一致。急性白血病的治疗一向以化疗为主,不同病人或不同病期对化疗药物的敏感性均不同 ...
苏勇庄
doaj +2 more sources
Contamination and mobile colistin resistance gene analysis for Salmonella isolated from retail chickens in Ji'nan [PDF]
, 2023 ObjectiveTo investigate the contamination of Salmonella in retail chickens in Ji’nan city from 2020 to 2021， and to explore the prevalence of the mobile colistin resistance gene （mcr） among these Salmonella isolates.MethodsFrom December 2020 to November ...
HU Yujie +7 more
core +1 more source
Pathogens and drug resistance in active surveillance of foodborne diseases from 2017 to 2019 in Chenzhou City [PDF]
, 2023 ObjectiveTo provide a scientific basis for the prevention and control of foodborne diseases in Chenzhou， the etiological characteristics and epidemiological patterns of foodborne diseases were analyzed.MethodsThe Case information and stool and anal swab ...
JIA Huayun +6 more
core +1 more source
Analysis of nontyphoidal Salmonella clinical isolates antibiotic resistance based on whole genome sequencing in Jiangxi Province in 2018 [PDF]
, 2020 Objective Understanding of bacterial antibiotic resistance is the basis for guiding clinical anti-infective therapy and monitoring antimicrobial resistance trends.
Chengwei LIU +7 more
core +1 more source
Infection status and etiological characteristics of diarrheogenic Escherichia coli among diarrhea patients in sentinel hospitals of Fujian Province in 2019 [PDF]
, 2020 Objective To investigate the infectious status, virulence gene, molecular typing and antibiotic resistance of diarrheogenic Escherichia coli (DEC) in sentinel hospitals of Fujian Province in 2019.
Haimei YE +4 more
core +1 more source
Rapid identification of drug resistance of Escherichia coli based on electrochemical square wave voltammetry of resazurin [PDF]
Objective: To evaluate the antibiotic resistance of five foodborne Escherichia coli clinical isolates to ciprofloxacin, gentamicin and ampicillin. Methods: Based on the theory of electron transfer during bacterial energy metabolism and the introduction ...
LU Lisheng +5 more
core +1 more source
野生动物学报, 2021 为了解卷羽鹈鹕（Pelecanus crispus）源大肠埃希菌（Escherichia coli）和粪肠球菌（Enterococcus faecalis）耐药性特征，采集圈养卷羽鹈鹕粪便9份，用全自动微生物生化鉴定法进行鉴定。结果表明：分别从9份粪便样中分离鉴定出大肠埃希菌9株，粪肠球菌7株。药敏试验结果显示，大肠埃希菌对氨苄西林、四环素和磺胺异恶唑的耐药性均较强，对阿莫西林/克拉维酸、头孢噻呋、多西环素等均较为敏感；粪肠球菌对青霉素、红霉素、四环素的耐药性均较强，对链霉素、呋喃妥因和氯霉素等较敏感 ...
张佰莲 1 马珺 2 刘群秀 2
doaj
Analysis of virulence genes and antimicrobial resistance of Vibrio parahaemolyticus isolated from shellfish in 2018 [PDF]
, 2020 Objective To analyze the virulence genes and antimicrobial resistance of 140 Vibrio parahaemolyticus strains from shellfish collected from farms and markets.
Caiyun LIN +8 more
core +1 more source
, 2012 从广东省以及海南省等地区养殖的患病罗非鱼体内分离、收集到多株致病菌,经生化分析和分子生物学鉴定,均为无乳链球菌。对这些菌株分别进行了耐药谱测定、分子分型试验以及分子血清型分析。药敏试验结果表明,2007—2010年分离到的无乳链球菌耐药谱基本相似;多位点可变数目串联重复序列分析(MLVA)试验中,选择5个高变异指数的可变数目重复位点(VNTR)进行分子分型,结果表明,所有鱼源无乳链球菌菌株为同一MLVA型,而作为对照的牛源无乳链球菌则明显不同;为了对这些菌株进一步分型 ...
张德锋 +5 more
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Multicolor Melting Curve Analysis-based on Detection of Pyrazinamide-Resistant Mycobacterium tuberculosis [PDF]
, 2017 结核病是由结核分枝杆菌感染人体的慢性传染性疾病。2015年新增患病人数高达1040万，有140万人死于结核病。在众多治疗结核病药物中，吡嗪酰胺是较早发现的一线抗结核药物之一。在酸性环境下，吡嗪酰胺可以抑制巨噬细胞内半休眠结核分枝杆菌。随着吡嗪酰胺广泛使用，耐受吡嗪酰胺的结核病危害面广，因此吡嗪酰胺耐药突变检测的研究急需开展。针对上述问题，本文利用探针熔解曲线分析技术对结核分枝杆菌吡嗪酰胺耐药突变检测展开研究。 第一章绪论，首先简要概述现今世界结核病概况，再介绍耐药结核病诊断和治疗的方法 ...
李梓
core