Results 111 to 120 of about 14,936 (183)

RNAi-chk1在抑制小鼠Lewis肺癌细胞生长作用的实验研究

Zhongguo shiyan zhenduanxue, 2012
目的观察chk1-RNAi联合放疗对Lewis肺癌细胞的抑制及凋亡情况。方法以脂质体Lipofectimine-2000介导,将chk1-536的siRNA表达载体转染小鼠Lewis肺癌细胞株,将其分为空白对照组、错义序列组、siRNA组,2Gy照射组、2Gy照射+错义序列组、2Gy照射+siRNA组。应用MTT法检测转染细胞抑制率、流式细胞术分析转染siRNA后细胞的凋亡,并应用RT-PCR检测转染细胞chk1-mRNA表达水平。结果 chk1-siRNA转染联合辐射组的Lewis肺癌细胞 ...
侯吉光, 张奇, 王红勇, 贾晓晶, 贾杰   +4 more
doaj  

人类免疫缺陷病毒-1 nef诱导凋亡及下调人类白细胞抗原-Ⅰ类分子

Zhongshan Daxue xuebao. Yixue kexue ban, 2007
[目的]研究人类免疫缺陷病毒（HIV）-1 nef调节细胞表面的人类白细胞抗原（HLA）-Ⅰ类分子及诱导细胞凋亡情况。[方法]将293T细胞分为两组,用脂质体法将含HIV-1 nef基因的质粒及空载体分别转染细胞,Western blot检测HIV-1 nef在转染细胞中的表达,荧光抗体染色结合流式细胞术检测细胞表面的HLA-I类分子表达及细胞凋亡情况。[结果]HIV-1 nef在转染细胞中有表达。转染HIV-1 nef的细胞与转染空载体的细胞经抗HLA-A,B,C-FITC染色后 ...
黄官友, 黄秀艳, 曾耀英, 曾祥凤, 吴晓萍   +4 more
doaj  

Hemodialysis bilayer bionic blood vessels developed by the mechanical stimulation of hepatitis B viral X(HBX) gene- transfected hepatic stellate cells. [PDF]

J Zhejiang Univ Sci B
Liu H, Zhou Y, Guo P, Zheng X, Chen W, Zhang S, Fu Y, Zhou X, Wan Z, Zhao B, Zhao Y.   +10 more
europepmc   +1 more source

rAAV1-EGFP和rAAV2-EGFP转染犬骨髓间充质 干细胞的比较

Zhongshan Daxue xuebao. Yixue kexue ban, 2009
【目的】 研究不同血清型腺相关病毒(rAAV)载体介导的增强绿色荧光蛋白基因(EGFP)对体外培养犬骨髓间充质干细胞(MSC)的转染效率及其毒性作用65377;【方法】 应用密度梯度离心法及贴壁筛选法分离培养犬MSC,倒置显微镜及Giemsa染色观察细胞形态,透射电镜观察其超微结构,流式细胞仪鉴定其表面标记物65377;取第3代MSC,以MOI为104 ~ 105 v.g./cell 的rAAV1-EGFP 和rAAV2-EGFP进行转染,3 d65380;7 d65380;21 ...
doaj  

[Effect of dephosphorylation of tumor metastasis suppressor gene <i>LASS2</i> on vacuolar ATPase activity and invasiveness of prostate cancer]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Liu Y, Lu M, Zhao X, Zhang K, Wu R, Mei F, Dai Z, You J, Pei F.   +8 more
europepmc   +1 more source

[The role and molecular mechanism of transcription factor EB and its target genes in multiple myeloma treatment with bortezomib]. [PDF]

Zhonghua Xue Ye Xue Za Zhi
Zhang RJ, Wang ZL, Shi XM, Zhang SY, Wang W, Ma MS, Li C, Gu CH, Zhang ZH.   +8 more
europepmc   +1 more source

["Chongqing experiences" in treatment of bone infections]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Xie Z.
europepmc   +1 more source

大鲵蛙病毒编码96L蛋白(ADRV-96L)的腺苷三磷酸酶活性和促进细胞生长作用 [PDF]

, 2018
张奇亚, 张锐
core  

[Aerobic exercise regulates macrophage polarization and improves insulin resistance in mice: the mediating role of miR-221-3p]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Li N, Zhang L, Guo Q, Zhou Y, Liu C.
europepmc   +1 more source

[Angio-associated migratory cell protein is highly expressed in hepatocellular carcinoma and promotes tumor cell invasion and metastasis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Li C, Yin G, Cheng X, Jiang Y.
europepmc   +1 more source