Results 31 to 40 of about 14,936 (183)
Zhongguo shiyan zhenduanxue, 2012 目的探讨klotho基因转染对脂肪间充质干细胞（ADSCs）增殖的影响。方法将含有klotho全长基因的真核质粒转染至从脂肪组织分离的ADSCs中,采用CCK-8法测定转染后细胞增殖率,western blot法检测转染后细胞周期蛋白D1的表达变化,流式细胞术检测转染前后ADSCs的细胞周期变化。结果转染klotho基因96h后AD-SCs的细胞增殖率为170.50%;流式细胞术检测结果显示,ADSCs转染klotho基因S期和G2期细胞明显增多;west-ern ...
韩冬, 徐煌, 郑永霞, 肖燕萍
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Cloning and expression patterns of stem cells induction-associated pluripotency factors in large yellow croaker Larimichthys crocea [PDF]
, 2016 通过iPSCs技术获得iPSCs，建立个体特异的多能干细胞系，可为生长发育、种质资源保护、疾病防治及药物筛选研究等提供宝贵的实验材料，是一条新的从根本上解决大黄鱼养殖中的各种难题的有效策略模式。其中，多能性因子对iPSCs技术的研究是必不可少的，它们在调控胚胎干细胞（ESCs）的自我更新和多能性、原始生殖细胞（PGCs）的形成、早期胚胎发育、生殖细胞发生、器官发生、再生及增殖、生长、分化、凋亡等细胞过程等方面发挥重要作用。然而关于大黄鱼多能性因子的研究尚属空白。本文采用SMART ...
姜永华
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Ultrasound in Obstetrics &Gynecology, Volume 66, Issue 5, Page 582-588, November 2025.ABSTRACT Objective The corpus callosum (CC) is an interhemispheric structure that facilitates communication between the two cerebral hemispheres. Anomalies of the CC are frequent and associated with a wide spectrum of altered neurodevelopmental outcomes.
R. Corroenne +55 more
wiley +1 more source
超声介导微泡破裂法对VEGF体外转染成纤维细胞蛋白表达影响的实验研究
Zhongguo shiyan zhenduanxue, 2008 目的利用ELISA检测法观察诊断剂量的超声介导微泡破裂法对VEGF体外转染成纤维细胞蛋白表达的影响并探讨该种转染方法的最佳超声条件。方法实验分组:单纯VEGF质粒转染组;超声+造影剂微泡+VEGF质粒转染组,该组并根据MI（机械指数）分为三组1.2;1.4;1.6。转染后利用ELISA法对各组成纤维细胞的VEGF蛋白表达进行定量检测;利用锥虫蓝染色法比较不同超声强度对细胞活性的影响。结果VEGF质粒转染成纤维细胞后,24h开始即有较高水平的VEGF蛋白表达,表达量于48 h7、2 h呈下降趋势;超声 ...
赵丽荣, 王小丛, 杨松青, 刘彬
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siRNA抑制人卵巢癌SKOV-3细胞MDM2的表达及细胞增殖的研究
Zhongguo shiyan zhenduanxue, 2021 目的研究siRNA对人卵巢癌细胞SKOV-3细胞MDM2基因表达的抑制作用及对肿瘤细胞增殖和凋亡的影响。方法体外合成2对针对MDM2基因的siRNA,经脂质体转染导入SKOV-3细胞;转染siRNA MDM2(简称si-MDM2)转染阴性对照质粒到SKOV-3,并设只加转染试剂作空白对照组,采用MTT法检测si-MDM2对细胞增殖的抑制作用。流式细胞仪检测细胞凋亡,并用Real-time PCR和Western blot分别从mRNA水平和蛋白水平检测si-MDM2对MDM2的抑制作用。结果 ...
王莹, 贾志勇, 赵燕颖
doaj
New Phytologist, Volume 247, Issue 5, Page 2086-2097, September 2025.Summary The importance of marine angiosperms, or seagrasses, as foundation species for marine coastal ecosystems is in marked contrast to their low species number of only 70 described taxa. Seagrass species tend to have very similar overall morphologies dictated by hydrodynamic forces of the ocean environment.
Xiaomei Zhang +14 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】探讨腺病毒介导血管内皮细胞生长因子(VEGF165)基因转染大鼠骨髓间充质干细胞(MSCs)后目的基因表达情况及对MSCs增殖分化的影响。【方法】构建含VEGF基因的腺病毒表达载体,利用贴壁法分离培养MSCs后,用荧光显微镜和流式细胞仪检测转染效果和转染率,用免疫组化、Western-Blot和ELISA方法分别检测VEGF基因转染MSCs后VEGF的表达情况。【结果】腺病毒介导的VEGF基因对于MSCs具有很高的转染效率,转染效率与病毒感染复制数(multiplicyties of ...
华平 +6 more
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Curcumin Promoted the Apoptosis of Cisplain-resistant Human Lung Carcinoma Cells A549/DDP through Down-regulating miR-186* [PDF]
, 2010 Background and objective Curcumin, a natural compound, is derived from the rthizom of Curcuma longa. In vitro and in vivo preclinical studies have shown its anti-inflammatory, antioxidant, anticancer activities and so on.
Jian ZHANG, Ni TANG, Yongping DU
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Veterinary Dermatology, Volume 36, Issue 3, Page 302-313, June 2025.Background – Malassezia pachydermatis (MP) is implicated in severe dermatitis and otitis externa (OE) of companion animals and recently gained attention for its increasing resistance to azole compounds. For this reason, developing novel therapeutic strategies is of great interest. In a previous work, we used reference yeast isolates to evaluate several
Costanza Spadini +11 more
wiley +1 more source
腺病毒介导的BMP-2基因转染骨髓基质干细胞及种植异种骨支架的体外观察
Zhongguo shiyan zhenduanxue, 2004 目的 用人骨形态发生蛋白 2腺病毒表达载体 （Ad BMP 2 ）转染的兔骨髓基质干细胞 （BMSC） ,种植BCB（去抗原牛松质骨 ）支架体外构建组织工程骨。方法 蛋白印迹法检测转染后细胞BMP 2的表达 ,ALP活性检测分析基因转染对细胞分化的影响。然后将转染后细胞接种到BCB支架上 ,扫描电镜观察细胞贴附、生长状况。结果 转染后 ,BMSC表达BMP 2 ,ALP活性明显增高。扫描电镜见转染细胞分布均匀 ,伸展良好。结论 Ad BMP 2可高效转染BM SC ,且促进细胞分化 ...
景元海 +4 more
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