Results 31 to 40 of about 8,139 (126)
Zhongguo shiyan zhenduanxue, 2007 目的构建HBsAg真核表达载体,转染CHO细胞并检测其表达。方法通过PCR法扩增出乙肝表面抗原的S基因和dhfr基因,分别克隆到T载体上,然后分别将S基因和dhfr基因克隆到pCI载体上,将pCI载体的CMV启动子替换为hEF-1α启动子。对新构建的pEF1-αS-dhfr真核表达载体进行酶切和测序鉴定。用脂质体法转染CHO（dhfr-）细胞,并用ELISA法检测HBsAg表达。结果hEF-1α启动子、S基因和dhfr基因经测序与Genbank报道一致。真核表达载体pEF1-αS ...
张艳, 时成波, 郭丽, 范洪学
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Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT The commercialization of lithium‐sulfur batteries (LSBs) faces dual challenges of sluggish sulfur redox kinetics and lithium polysulfides (LiPSs) shuttling. Herein, we demonstrate a synergistic strategy combining multidimensional structural confinement with d‐band center modulation to simultaneously suppress LiPSs diffusion and catalyze ...
Shibo Rong +8 more
wiley +1 more source
基于episomal CRISPR/Cas9 载体的基因敲入方法
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】研究一种在哺乳动物细胞中高效的基因敲入方法，比较带有oriP-EBNA1 序列的episomal CRISPR/Cas9载体与普通CRISPR/Cas9载体的基因敲入效率是否有差别。【方法】首先利用慢病毒载体构建稳定表达红色荧光蛋白DsRedE2的人胚肾细胞系（293FT）与人诱导多能干细胞系（hiPS）；利用CRISPR/Cas9序列设计网站，设计和合成靶向DsRedE2的向导RNA（sgRNA），分别克隆至带有oriP-EBNA1的episomal CRISPR ...
邹征伟, 赖兴强, 李伟强
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Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT Light‐activated gas sensors hold significant potential in environmental monitoring and smart Internet of Things (IoT) owing to their advantage in room temperature operation. Nevertheless, due to a narrow light absorption range, high photocarrier recombination rate, and weak redox activity of the sensing material, the performance enhancement of
Jinyong Hu +5 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2011 目的构建（BbxⅡ）基因真核表达载体,为研究家蚕素Ⅱ在哺乳动物细胞中的生物学作用奠定基础。方法以含有BbxⅡcDNA的PUC57质粒为模板,采用聚合酶链式反应（PCR）扩增BbxⅡcDNA片段。真核表达载体PcD-NA3.1及PCR产物经双酶切后连接,转化大肠杆菌DH-5α感受态菌,获得重组载体PcDNA3.1-Bbx/His,进行酶切鉴定和测序鉴定。结果 PCR获得与预期大小一致约300 bp的特异性DNA,重组载体PcDNA3.1-BbxⅡ/His经双酶切及测序鉴定证实 ...
李洪霞 +5 more
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Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT Bone defect repair in elderly patients is frequently compromised by reduced osteogenic capacity and delayed healing. Chronic inflammation‐driven inflammaging is a critical contributor to this impairment, characterized by dysfunctional autophagy in senescent macrophages (S‐MΦs), excessive reactive oxygen species (ROS) accumulation, and ...
Ruiyue Hang +6 more
wiley +1 more source
Gongye shui chuli, 2012 通过悬挂式自然供氧工艺,分别采用聚对苯二甲酸乙二醇酯、聚酰胺6和聚丙烯腈3种纤维材质的绳式生物膜载体对COD为(400±5)mg/L的果糖废水进行处理,比较了3种生物膜载体的挂膜效率。结果表明,聚对苯二甲酸乙二醇酯纤维材质生物膜载体挂膜效率﹥聚酰胺6纤维材质生物膜载体挂膜效率﹥聚丙烯腈纤维材质生物膜载体挂膜效率。在温度为20～25℃条件下,运行10.42 h后,聚对苯二甲酸乙二醇酯纤维材质生物膜载体下端5 m处出现1.0 mm厚度的生物膜。
刘景涛 +5 more
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The development of metallic catalysts for Suzuki cross-coupling reaction(应用于Suzuki偶联反应的金属催化剂研究进展)
Zhejiang Daxue xuebao. Lixue ban, 2018 Suzuki偶联反应是用于选择性构建C－C键的最通用、最有效的反应之一，特别是用于形成联芳基时非常有效.应用于Suzuki偶联反应的金属催化剂可分为均相和非均相催化剂两大类，均相催化剂的金属配体对催化性能有极强的影响；而非均相催化剂，对催化性能有重要影响的是载体.首先介绍了膦配体与氮配体，分别讨论了其催化活性，其中有些配体展现了极高的性能.还讨论了 MOFs聚合物等传统载体和新型载体.最后展望了配体与载体在Suzuki偶联反应中的应用前景.
HULingjun(胡玲君) +1 more
doaj +1 more source
New Phytologist, Volume 251, Issue 1, Page 207-227, July 2026.Network of VAMP714‐dependent interactions during salt stress. Summary Plant responses to salt stress include an altered root architecture mediated by ion imbalances, reactive oxygen species (ROS) accumulation, and altered hormonal responses. Auxin is implicated in these processes.
Jialei Sun +3 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2014 目的构建Tet-on调控的人HOXB4慢病毒载体,为体外扩增造血干细胞并保持其干细胞特性提供实验材料基础。方法应用PCR技术从质粒TAT-HA-HOXB4-Full中扩增编码人HOXB4的cDNA,用限制性内切酶EcoRI分别酶切HOXB4基因片段和带有Tet-on开关的可调控慢病毒载体,经T4DNA连接酶连接获得慢病毒载体Teto-Fuw-hHOXB4。对构建的HOXB4慢病毒载体进行酶切片段凝胶电泳及DNA测序分析。结果酶切片段电泳分析和DNA测序分析证明Tet ...
李秀英 +5 more
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