Results 31 to 40 of about 481,638 (177)

人β防御素-2基因的克隆及原核表达载体的构建

open access: yesZhongguo shiyan zhenduanxue, 2006
目的构建人β-防御素2的融合性原核表达载体,为今后基因工程手段表达纯化及生产人β-防御素-2奠定基础。方法体外基因合成人β-防御素2(HBD-2)的基因片段,并克隆入pMD18T中间载体中,经酶切鉴定后再克隆入pET32a原核表达载体中,与载体中所固有的TrX-A形成融合基因。结果构建的融合性原核表达载体经酶切鉴定无突变发生。结论成功地构建了pET-32a-(HBD-2)。
魏洪涛, 董震, 张国利
doaj  

Electrospinning Synthesized Cd0.3Zn0.7S@PVDF/PAN Piezoelectric Nanofiber Membrane for Efficient Photocatalytic Hydrogen Evolution

open access: yesRare Metals, Volume 45, Issue 4, April 2026.
ABSTRACT Photocatalytic hydrogen evolution technology is a crucial approach to achieve efficient solar energy conversion and green hydrogen production. However, traditional semiconductor photocatalysts often face issues such as weak visible‐light response, high photogenerated charge carrier recombination, and poor structural stability, severely ...
Longtao Guo   +8 more
wiley   +1 more source

选择加氢催化剂载体、其制备方法及含该载体的催化剂

open access: yes, 2004
本发明涉及芳烃选择加氢制备环烯烃的钌基催化剂的载体、其制备方法及含该载体的催化剂。加氢催化剂由钌和氧化物载体组成。载体由过渡金属氧化物和其它主族的氧化物组成,由共沉淀法制备。采用本发明的催化剂载体可以节约钌的用量,降低成本,并能使催化剂的效率和寿命得到提高。该催化剂对芳烃选择加氢制备环烯烃具有高转化率和高选择性 ...
傅送保   +5 more
core  

基于episomal CRISPR/Cas9 载体的基因敲入方法

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2018
【目的】研究一种在哺乳动物细胞中高效的基因敲入方法,比较带有oriP-EBNA1 序列的episomal CRISPR/Cas9载体与普通CRISPR/Cas9载体的基因敲入效率是否有差别。【方法】首先利用慢病毒载体构建稳定表达红色荧光蛋白DsRedE2的人胚肾细胞系(293FT)与人诱导多能干细胞系(hiPS);利用CRISPR/Cas9序列设计网站,设计和合成靶向DsRedE2的向导RNA(sgRNA),分别克隆至带有oriP-EBNA1的episomal CRISPR ...
邹征伟, 赖兴强, 李伟强
doaj  

Investigating the Origin of Topological‐Hall‐Like Resistivity in Zn‐Doped Mn2Sb Ferrimagnet

open access: yesRare Metals, Volume 45, Issue 4, April 2026.
ABSTRACT Skyrmions and other chiral spin textures have been extensively studied as potential building blocks for novel spintronic devices. Hall‐resistivity anomalies that deviate from magnetization scaling, known as the topological Hall effect, have been widely employed as evidence for the presence of chiral spin textures in magnetic materials. However,
BoCheng Yu   +9 more
wiley   +1 more source

球形复旋转生物载体

open access: yes, 2000
本实用新型属微生物法处理污水的技术领域。是一种球形复旋转生物载体。其球形整体和内球均可旋转,在流动水体中可对水体和水中的氧气作重复剪切,故使水体中溶解氧 增加,同时,这种载体比表面积大,孔隙率高,易挂膜和脱膜,不堵塞,因而生化效率高。这种载体抗腐蚀,抗冲击,强度高,使用方便, 使用寿命长,成本低廉 ...
李福德, 田崇民, 王仲文, 李昕
core  

Ultrahigh‐Strength Soft‐Magnetic NiFeCoAl Multi‐Principal Element Alloys Enabled by Multiscale Heterostructure Design

open access: yesRare Metals, Volume 45, Issue 4, April 2026.
ABSTRACT Soft‐magnetic materials (SMMs) are indispensable for electrification and sustainable energy systems, yet their inherent mechanical fragility fundamentally limits applications under severe mechanical stresses. Enhancing the yield strength of SMMs is essential to prevent the degradation in magnetic performance and failure from plastic ...
Nairan Wang   +6 more
wiley   +1 more source

HvABA8′OH-1基因RNAi载体的构建及遗传转化

open access: yes, 2010
穗发芽严重影响作物的产量和品质,种子休眠性弱是穗发芽形成的主要原因之一.ABA是维持胚休眠抑制萌发的重要激素,大麦HvABA8′OH-1基因编码控制内源ABA主要分解代谢途径的关键酶.以双元载体pGreenⅡ为骨架载体,分别选取HvABA8′OH-1的保守区序列(293bp)和特异区序列(474bp),水稻胚乳特异性启动子pGlub,成功构建了种子特异性RNA干扰表达载体pOHC和pOHS.利用农杆菌介导法将载体转入水稻,获得植株50株.经鉴定,其中18株为转基因阳性植株 ...
郑寒   +5 more
core  

家蚕素Ⅱ基因真核表达载体的构建

open access: yesZhongguo shiyan zhenduanxue, 2011
目的构建(BbxⅡ)基因真核表达载体,为研究家蚕素Ⅱ在哺乳动物细胞中的生物学作用奠定基础。方法以含有BbxⅡcDNA的PUC57质粒为模板,采用聚合酶链式反应(PCR)扩增BbxⅡcDNA片段。真核表达载体PcD-NA3.1及PCR产物经双酶切后连接,转化大肠杆菌DH-5α感受态菌,获得重组载体PcDNA3.1-Bbx/His,进行酶切鉴定和测序鉴定。结果 PCR获得与预期大小一致约300 bp的特异性DNA,重组载体PcDNA3.1-BbxⅡ/His经双酶切及测序鉴定证实 ...
李洪霞   +5 more
doaj  

The development of metallic catalysts for Suzuki cross-coupling reaction(应用于Suzuki偶联反应的金属催化剂研究进展)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2018
Suzuki偶联反应是用于选择性构建C-C键的最通用、最有效的反应之一,特别是用于形成联芳基时非常有效.应用于Suzuki偶联反应的金属催化剂可分为均相和非均相催化剂两大类,均相催化剂的金属配体对催化性能有极强的影响;而非均相催化剂,对催化性能有重要影响的是载体.首先介绍了膦配体与氮配体,分别讨论了其催化活性,其中有些配体展现了极高的性能.还讨论了 MOFs聚合物等传统载体和新型载体.最后展望了配体与载体在Suzuki偶联反应中的应用前景.
HULingjun(胡玲君)   +1 more
doaj   +1 more source

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