Results 21 to 30 of about 631,710 (162)
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT Photocatalytic acetylene (C2H2) semihydrogenation is a green route for ethylene (C2H4) production, yet the activity–selectivity trade‐off caused by imbalanced adsorption/activation of reactants and products remains a core challenge. Herein, we realize precise regulation of Pd oxidation states (Pd0, Pdδ+, and Pd2+) on anatase TiO2 nanocubes via
Zhidan Wang +7 more
wiley +1 more source
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT The immunosuppressive milieu of hepatocellular carcinoma (HCC) remains a major obstacle to durable antitumor responses. Mechanical‐energy‐driven piezocatalysis provides a noninvasive means of inducing oxidative damage and immunogenic tumor destruction; however, inefficient utilization of piezoinduced charges and insufficient localization of ...
Mingjian Zhang +7 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2010 目的构建并筛选到能有效抑制TLR4基因表达的小干扰RNA表达载体,用于研究TLR4基因功能。方法 RNA干扰采用体外构建小干扰RNA表达载体法,将构建好的表达载体转染单核细胞株,分别用流式细胞术和实时荧光定量RT-PCR法检测TLR4蛋白和mRNA表达变化,筛选抑制效率最高的小干扰RNA表达载体。结果成功构建了TLR4小干扰RNA表达载体,并经电泳及测序证实;编号为S8-1的载体转染入细胞后,TLR4阳性率为27.03%,显著低于对照组的35.12%;流式细胞术和实时荧光定量RT ...
耿红莲 +6 more
doaj
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT Bimetallic structures fabricated by laser powder bed fusion (LPBF) provide an effective strategy for achieving multifunctional integration, yet interfacial structural continuity and the role of lattice defects remain insufficiently understood.
Jinguo Ge, Shuo Yin
wiley +1 more source
Gongye shui chuli, 2012 通过悬挂式自然供氧工艺,分别采用聚对苯二甲酸乙二醇酯、聚酰胺6和聚丙烯腈3种纤维材质的绳式生物膜载体对COD为(400±5)mg/L的果糖废水进行处理,比较了3种生物膜载体的挂膜效率。结果表明,聚对苯二甲酸乙二醇酯纤维材质生物膜载体挂膜效率﹥聚酰胺6纤维材质生物膜载体挂膜效率﹥聚丙烯腈纤维材质生物膜载体挂膜效率。在温度为20～25℃条件下,运行10.42 h后,聚对苯二甲酸乙二醇酯纤维材质生物膜载体下端5 m处出现1.0 mm厚度的生物膜。
刘景涛 +5 more
doaj +2 more sources
Zhongguo shiyan zhenduanxue, 2010 目的探讨慢病毒载体在CD34+脐血细胞（CBCs）中的基因转导效率,为基因治疗的临床应用提供关键材料。方法应用1型人类免疫缺陷病毒（HIV-1）改造而成的第三代自身失活（self-inactivating,SIN）慢病毒载体（lentiviralvector）系统,通过流式细胞术检测基因导入细胞百分比,评价该载体系统在人CBCs中的基因转导效率。结果 HIV载体的转导效率在95%以上。结论基于慢病毒载体基因转导的高效性,该载体系统可作为CD34+CBCs基因转导的极好工具。
卢振霞, 白元松, 林玉梅
doaj
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT High‐yield photocatalytic H2O2 production in sacrificial agent‐free pure water constitutes a critical bottleneck requiring urgent advances in energy catalysis. Although ZnIn2S4 (ZIS) exhibits favourable visible light absorption, it is plagued by severe bulk charge carrier recombination and scarce accessible surface‐active sites.
Sheng Liu +7 more
wiley +1 more source
柯萨奇病毒B4 2B基因siRNA表达载体的构建及其对柯萨奇病毒B4抑制作用的研究
Zhongguo shiyan zhenduanxue, 2006 目的构建针对柯萨奇病毒B4 2B基因的siRNA表达载体并检测该载体在体外培养细胞中对柯萨奇病毒B4的抑制作用。方法选择柯萨奇病毒B4的2B基因区21bp的基因片段作为靶序列,合成含有靶序列的DNA片段并克隆到pGCsi-U6/Neo/GFP/siNeGative载体中,构建pGCsi-U6/Neo/GFP/2B,使其可表达具有发夹结构的双链siR-NA,在转染该载体后用柯萨奇病毒B4攻击Hela细胞,检测该载体对病毒感染细胞的保护效应。结果通过酶切、电泳、序列分析及荧光显微镜观察证明载体构建成功 ...
史红艳 +4 more
doaj
Integrative Conservation, Volume 5, Issue 3, Page 255-267, September 2026.This study presents a comprehensive review of the 500‐year spatiotemporal evolution of global botanical gardens, identifying three major development stages—initial exploration (1543–1699), steady expansion (1700–1899), and rapid proliferation (1900–2020)—with six distinct sub‐stages. We propose a tripartite driving framework grounded in colonial botany
Shidong Li, Guangzhen Shi, Qingwei Li
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2011 目的构建卵巢特异启动子-1（ovarian-specific promoter,OSP-1）调控白介素-24基因（Interleukin-24,IL-24）表达的真核表达载体。方法通过RT-PCR方法从人外周血基因组中扩增IL-24基因片段,应用基因重组技术将0SP-1启动子片段插入到pcDNA3.0真核表达载体,替换pcDNA3.0载体的CMV启动子与增强子序列,然后将克隆的IL-24基因片段插入到pcDNA3.0-OSP-1载体的OSP-1启动子的下游,从而构建成由OSP-1启动子调控IL ...
祝贺 +4 more
doaj