Results 21 to 30 of about 8,139 (126)
Zhongguo shiyan zhenduanxue, 2010 目的探讨慢病毒载体在CD34+脐血细胞（CBCs）中的基因转导效率,为基因治疗的临床应用提供关键材料。方法应用1型人类免疫缺陷病毒（HIV-1）改造而成的第三代自身失活（self-inactivating,SIN）慢病毒载体（lentiviralvector）系统,通过流式细胞术检测基因导入细胞百分比,评价该载体系统在人CBCs中的基因转导效率。结果 HIV载体的转导效率在95%以上。结论基于慢病毒载体基因转导的高效性,该载体系统可作为CD34+CBCs基因转导的极好工具。
卢振霞, 白元松, 林玉梅
doaj
Rare Metals, Volume 45, Issue 8, August 2026.ABSTRACT A central challenge in alkaline water electrolysis is the development of hydrogen evolution reaction (HER) electrocatalysts that simultaneously maintain high activity and structural stability at industrial current densities. Hydroxide‐based supports that facilitate water dissociation are particularly susceptible to over‐reduction and ...
Feng Chen +9 more
wiley +1 more source
柯萨奇病毒B4 2B基因siRNA表达载体的构建及其对柯萨奇病毒B4抑制作用的研究
Zhongguo shiyan zhenduanxue, 2006 目的构建针对柯萨奇病毒B4 2B基因的siRNA表达载体并检测该载体在体外培养细胞中对柯萨奇病毒B4的抑制作用。方法选择柯萨奇病毒B4的2B基因区21bp的基因片段作为靶序列,合成含有靶序列的DNA片段并克隆到pGCsi-U6/Neo/GFP/siNeGative载体中,构建pGCsi-U6/Neo/GFP/2B,使其可表达具有发夹结构的双链siR-NA,在转染该载体后用柯萨奇病毒B4攻击Hela细胞,检测该载体对病毒感染细胞的保护效应。结果通过酶切、电泳、序列分析及荧光显微镜观察证明载体构建成功 ...
史红艳 +4 more
doaj
Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT Because of superior transportability and high energy capacity, methanol is regarded as a potential hydrogen carrier. Photocatalytic methanol dehydrogenation offers a sustainable and cost‐effective method for releasing hydrogen. Conventional photocatalysts face challenging obstacles, including the low catalytic efficiency and rapid ...
Rundong Chen +9 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2011 目的构建卵巢特异启动子-1（ovarian-specific promoter,OSP-1）调控白介素-24基因（Interleukin-24,IL-24）表达的真核表达载体。方法通过RT-PCR方法从人外周血基因组中扩增IL-24基因片段,应用基因重组技术将0SP-1启动子片段插入到pcDNA3.0真核表达载体,替换pcDNA3.0载体的CMV启动子与增强子序列,然后将克隆的IL-24基因片段插入到pcDNA3.0-OSP-1载体的OSP-1启动子的下游,从而构建成由OSP-1启动子调控IL ...
祝贺 +4 more
doaj
Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT Biodegradable zinc (Zn)‐based materials are considered promising candidates for orthopedic applications because of their favorable mechanical properties, controllable biodegradability, and good biosafety profiles. However, their clinical translation is limited by early‐stage corrosion‐induced cytotoxicity and inflammation, as well as ...
Jinlong Mao +9 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】建立稳定miR-18a过表达及表达的人鼻咽癌细胞系，为研究miR-18a在鼻咽癌发生发展中 的功能奠定基础。【方法】将miR-18a前体的编码基因片段及miR-18a反义寡核苷酸克隆到慢病毒载体中，DNA 测序鉴定；将构建好的载体转染293T细胞，获得重组miR-18a过表达及抑制表达的慢病毒载体，感染人鼻咽癌细 胞CNE1和CNE2，嘌呤霉素筛选获得稳定干扰miR-18a表达及过表达miR-18a的细胞系，PCR及western blot鉴 定。【结果】成功构建真核表达的慢病毒miR ...
常利红 +5 more
doaj
Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT The composition and structural characteristics of metacomposites are crucial for tuning their negative electromagnetic parameters; however, the lack of a clear structure–property relationship severely limits their application in dielectric and magnetic fields.
Chunyuan Deng +9 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2006 目的构建人β-防御素2的融合性原核表达载体,为今后基因工程手段表达纯化及生产人β-防御素-2奠定基础。方法体外基因合成人β-防御素2（HBD-2）的基因片段,并克隆入pMD18T中间载体中,经酶切鉴定后再克隆入pET32a原核表达载体中,与载体中所固有的TrX-A形成融合基因。结果构建的融合性原核表达载体经酶切鉴定无突变发生。结论成功地构建了pET-32a-（HBD-2）。
魏洪涛, 董震, 张国利
doaj
Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT Mechanical dynamic sealing components are usually subjected to the erosion of a hydrothermal environment; thus, the selected service materials must meet stringent requirements for superior self‐lubrication, corrosion resistance, and thermal stability.
Yanchun Zhao +8 more
wiley +1 more source