Results 21 to 30 of about 635,304 (152)
Zhongguo shiyan zhenduanxue, 2010 目的探讨慢病毒载体在CD34+脐血细胞（CBCs）中的基因转导效率,为基因治疗的临床应用提供关键材料。方法应用1型人类免疫缺陷病毒（HIV-1）改造而成的第三代自身失活（self-inactivating,SIN）慢病毒载体（lentiviralvector）系统,通过流式细胞术检测基因导入细胞百分比,评价该载体系统在人CBCs中的基因转导效率。结果 HIV载体的转导效率在95%以上。结论基于慢病毒载体基因转导的高效性,该载体系统可作为CD34+CBCs基因转导的极好工具。
卢振霞, 白元松, 林玉梅
doaj
Zhejiang Daxue xuebao. Lixue ban, 2009 采用水热合成法制备了不锈钢负载的ZSM-5分子筛涂层.考察了不锈钢载体表面处理对分子筛与载体之间结合力的影响.研究结果表明,与酸处理或洗涤剂清洗处理相比,高温热处理更加有利于提高分子筛与不锈钢载体之间的结合力,且热处理效果与温度有关,最佳处理温度为850 ℃,在该温度下处理5 h后的载体,所得到的分子筛/不锈钢复合材料,不仅具有最大的分子筛表面覆盖率,而且在焙烧过程中分子筛几乎不脱落.热处理后的载体在反应液中进一步浸泡同样会影响分子筛的覆盖率及结合力,影响大小与载体热处理温度有关.
YUANHao(苑昊) +2 more
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Rare Metals, Volume 45, Issue 9, September 2026.ABSTRACT Ti‐15Mo‐2.5Ag alloy exhibited a low modulus and strong antibacterial ability but poor bioactivity. To resolve this problem, sandblasting and anodization were used to create a hydrophilic and nano‐Ag2O‐containing TiO2 coating. Subsequently, the microstructure and physicochemical properties were investigated by scanning electron microscopy, X ...
Yanchun Xie +11 more
wiley +1 more source
柯萨奇病毒B4 2B基因siRNA表达载体的构建及其对柯萨奇病毒B4抑制作用的研究
Zhongguo shiyan zhenduanxue, 2006 目的构建针对柯萨奇病毒B4 2B基因的siRNA表达载体并检测该载体在体外培养细胞中对柯萨奇病毒B4的抑制作用。方法选择柯萨奇病毒B4的2B基因区21bp的基因片段作为靶序列,合成含有靶序列的DNA片段并克隆到pGCsi-U6/Neo/GFP/siNeGative载体中,构建pGCsi-U6/Neo/GFP/2B,使其可表达具有发夹结构的双链siR-NA,在转染该载体后用柯萨奇病毒B4攻击Hela细胞,检测该载体对病毒感染细胞的保护效应。结果通过酶切、电泳、序列分析及荧光显微镜观察证明载体构建成功 ...
史红艳 +4 more
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Advances in Thermoelectric Cu2ZnSnS4: From Structure, Synthesis to Performance
Rare Metals, Volume 45, Issue 8, August 2026.ABSTRACT Thermoelectric technology has the capacity to directly convert waste heat into electricity and vice versa. For a long time, Cu2ZnSnS4 (CZTS) has been extensively researched in the domains of solar cells and photocatalysis due to its exceptional photovoltaic properties, but its wide bandgap prevents it from being favored in thermoelectrics ...
Xiaojun Li +3 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2007 目的构建HBsAg真核表达载体,转染CHO细胞并检测其表达。方法通过PCR法扩增出乙肝表面抗原的S基因和dhfr基因,分别克隆到T载体上,然后分别将S基因和dhfr基因克隆到pCI载体上,将pCI载体的CMV启动子替换为hEF-1α启动子。对新构建的pEF1-αS-dhfr真核表达载体进行酶切和测序鉴定。用脂质体法转染CHO（dhfr-）细胞,并用ELISA法检测HBsAg表达。结果hEF-1α启动子、S基因和dhfr基因经测序与Genbank报道一致。真核表达载体pEF1-αS ...
张艳, 时成波, 郭丽, 范洪学
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Rare Metals, Volume 45, Issue 8, August 2026.ABSTRACT A facile coprecipitation‐pyrolysis strategy was employed to fabricate Cu/Co nanoparticles (NPs) embedded in nitrogen‐doped carbon matrices (Cu/Co@N‐C) through the carbonization of CuCo‐MOF precursors. Moreover, monometallic counterparts of Cu@N‐C and Co@N‐C were also prepared via carbonization of bimetallic CuZn‐MOF and CoZn‐MOF, respectively.
Weinan Yao +7 more
wiley +1 more source
, 2004 本发明涉及芳烃选择加氢制备环烯烃的钌基催化剂的载体、其制备方法及含该载体的催化剂。加氢催化剂由钌和氧化物载体组成。载体由过渡金属氧化物和其它主族的氧化物组成，由共沉淀法制备。采用本发明的催化剂载体可以节约钌的用量，降低成本，并能使催化剂的效率和寿命得到提高。该催化剂对芳烃选择加氢制备环烯烃具有高转化率和高选择性 ...
傅送保 +5 more
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Zhongguo shiyan zhenduanxue, 2011 目的构建卵巢特异启动子-1（ovarian-specific promoter,OSP-1）调控白介素-24基因（Interleukin-24,IL-24）表达的真核表达载体。方法通过RT-PCR方法从人外周血基因组中扩增IL-24基因片段,应用基因重组技术将0SP-1启动子片段插入到pcDNA3.0真核表达载体,替换pcDNA3.0载体的CMV启动子与增强子序列,然后将克隆的IL-24基因片段插入到pcDNA3.0-OSP-1载体的OSP-1启动子的下游,从而构建成由OSP-1启动子调控IL ...
祝贺 +4 more
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Efficient Photocatalytic Removal of Toluene by Synergistic FeCe/TiO2: Performance and Mechanism
Rare Metals, Volume 45, Issue 8, August 2026.ABSTRACT Efficient removal of volatile organic compounds (VOCs) in confined spaces is crucial for environments such as spacecraft and deep‐sea submarines. Metal modification is an effective strategy to enhance the light absorption capacity of titanium dioxide (TiO2) and suppress the recombination of photogenerated charge carriers.
Ke Xu +10 more
wiley +1 more source