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Integrative Conservation, Volume 4, Issue 3, Page 277-286, September 2025.This study provides an innovation to address the challenge in accessibility to samples and genetic markers in Snow leopard conservation genetic studies. We performed next‐generation sequencing (NGS) on 19 fecal samples obtained from the eastern Qinghai‐Tibet Plateau, and successfully assembled 17 complete mitogenome sequences (~16,720 bp) and ...
Chen Cheng +7 more
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, 2012 目的筛选稀有鮈鲫HAN近交系遗传质量检测标记。方法采用鳞片活体移植和同工酶聚丙烯酰胺凝胶电泳法对稀有鮈鲫HAN近交系的遗传纯度进行检测。结果在免疫标记分析中,鳞片同体移植存活率为96.7%以上,野生群体移植存活率为7.4%,而HAN系F22鳞片异体移植的成功率为80%,显著高于野生群体。在生化标记分析中,在HAN系F22中无多态性位点,不同个体的同工酶谱呈现高度一致,在野生群体中有2个多态位点即est2和est3,多态位点的比例为15.56%。结论经过多代近亲交配 ...
曹文宣, 王伟, 王剑伟, 邵燕
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The Two Faces of Introgression in Conservation Biology 基因渗入在保育生物学中的双重面貌
Integrative Conservation, Volume 4, Issue 3, Page 339-344, September 2025.Conservation biologist need to take into account the various aspects of introgression in their attempts to protect the species integrity and evolutionary potential of threatened species. To do so, they have to overcome the bias created by the Mayrian species concept that considers species as having distinct gene pools.
Harald Schneider, Charles H. Cannon
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, 1991 本文介绍我室承担国家自然科学基金资助该课题的研究成果(1987～1990年);①系统地研究了精液/血液中PGM_I亚型、GLOⅠ,阴道液/血液中PGM_I亚型、GLQI、ESD等同工酶遗传多态性的相关性;②精浆和无精子精液的同工酶的活性;③月经周期和性刺激对阴道液上述同工酶活性的影响;④上述同工酶型与某些男女性生殖系统常见疾病的相关性 ...
伍新尧
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Application of miniSTR Loci and Its Detection System for Degraded Materials in Forensic Medicine#br# [PDF]
, 2018 Objective To establish multiplex system of 16 miniSTR loci, and explore its application value for the degraded materials in forensic medicine. Methods The multiplex system of 16 miniSTR loci was established using a six-dye fluorescence labeling ...
WANG Xin, CHEN Wei-zhong, ZHANG Jian, et al.
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Research Progress on Complex Kinship Analysis [PDF]
, 2020 Complex kinship analysis refers to a kind of special kinship analysis taken for the purpose of personal identification or other issues in civil or criminal cases because the father or （and） mother is dead, or cannot participate in the analysis for other ...
LI Mei, LI Shi-ying, ZHANG Bei-bei, et al.
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New Phytologist, Volume 247, Issue 5, Page 2086-2097, September 2025.Summary The importance of marine angiosperms, or seagrasses, as foundation species for marine coastal ecosystems is in marked contrast to their low species number of only 70 described taxa. Seagrass species tend to have very similar overall morphologies dictated by hydrodynamic forces of the ocean environment.
Xiaomei Zhang +14 more
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野生动物学报, 2018 为探索东北地区野猪种群的遗传资源特征,本研究通过收集长白山北部林区5个地点的140份粪便样本,结合世界粮农组织(FAO)推荐的9个微卫星标记,使用Microsatellite Toolkit、GENEALEx软件计算相关遗传参数,评估各野猪局域种群的遗传多样性。研究结果表明,9个微卫星标记检测到的等位基因数目为6～15个,且PIC值为0.625～0.888,均为高度多态性位点。种群平均表观杂合度为0.760～0.813,平均期望杂合度为0.687～0.766,平均有效等位基因数为3.368～5.144个,
葛宝庆 胡素贤 孙铁铎 张崇颖 姜广顺
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PCR/异源双链形成分析法在β地中海贫血产前诊断中的应用 [PDF]
, 1993 采用聚合酶链反应(PCR)结合聚丙烯酰胺凝胶电泳(PAGE),检测PCR产物异源双链形成情况,诊断和产前诊断B珠蛋白生成障碍性贫血(β地中海贫血)CD41-42(-CTTT)突变。此方法简便快速,可区分有无突变,并能鉴别纯合子与杂合子。应用本方法进行了4例产前诊断 ...
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Association of MTHFR C677T polymorphism with male infertile in Xiamen area [PDF]
, 2017 目的探讨亚甲基四氢叶酸还原酶（MTHFR）C677T基因多态性与男性不育的相关性。方法选取少、弱精症患者66例和无精症患者74例为实验组,精液正常且有正常孕育史的男性96例为对照组,分析MTHFR C677T基因多态性在三组中分布的差异。结果与对照组相比,少、弱精组中MTHFR C677T各基因型的频率差异没有显著性意义（P〉0.05）;与对照组相比,无精组中MTHFR C677T各基因型的频率差异没有显著性意义（P〉0.05）。结论在少、弱精症患者、无精症患者和健康人中,基因多态性分布无显著差异 ...
王厚照, 谭振军, 马芳芳
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