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16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences [PDF]

open access: yesFrontiers in Bioinformatics, 2022
Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh   +9 more
doaj   +2 more sources

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich   +9 more
doaj   +2 more sources

The 16S ribosomal RNA mutation database (16SMDB) [PDF]

open access: greenNucleic Acids Research, 1996
The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenotype has been detected by in vivo or ...
Kathleen L. Triman
openalex   +6 more sources

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]

open access: yesPLoS ONE, 2015
Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt   +8 more
doaj   +5 more sources

Reading canonical and modified nucleobases in 16S ribosomal RNA using nanopore native RNA sequencing.

open access: goldPLoS ONE, 2019
The ribosome small subunit is expressed in all living cells. It performs numerous essential functions during translation, including formation of the initiation complex and proofreading of base-pairs between mRNA codons and tRNA anticodons.
Andrew M Smith   +4 more
doaj   +2 more sources

Classification of methanogenic bacteria by 16S ribosomal RNA characterization [PDF]

open access: bronzeProceedings of the National Academy of Sciences, 1977
The 16S ribosomal RNAs from 10 species of methanogenic bacteria have been characterized in terms of the oligonucleotides produced by T 1 RNase digestion. Comparative analysis of these data reveals the methanogens to constitute a distinct phylogenetic group containing two major divisions. These organisms appear to be
George E. Fox   +4 more
openalex   +4 more sources

Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]

open access: yesScientific Reports, 2021
We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish   +3 more
doaj   +2 more sources

Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa [PDF]

open access: yesFrontiers in Microbiology
IntroductionShort read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota.
Arunabh Sharma   +10 more
doaj   +2 more sources

A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]

open access: yesScientific Reports
Over half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan   +5 more
doaj   +2 more sources

Selection of Peptides Targeting Helix 31 of Bacterial 16S Ribosomal RNA by Screening M13 Phage-Display Libraries [PDF]

open access: goldMolecules, 2011
Ribosomal RNA is the catalytic portion of ribosomes, and undergoes a variety of conformational changes during translation. Structural changes in ribosomal RNA can be facilitated by the presence of modified nucleotides. Helix 31 of bacterial 16S ribosomal
Christine S. Chow   +4 more
doaj   +2 more sources

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