Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich +9 more
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Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]
Scientific Reports, 2021 We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish +3 more
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Reading canonical and modified nucleobases in 16S ribosomal RNA using nanopore native RNA sequencing. [PDF]
PLoS ONE, 2019 The ribosome small subunit is expressed in all living cells. It performs numerous essential functions during translation, including formation of the initiation complex and proofreading of base-pairs between mRNA codons and tRNA anticodons.
Andrew M Smith +4 more
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Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene [PDF]
BMC Bioinformatics, 2018 Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo +9 more
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Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]
PLoS ONE, 2015 Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt +8 more
doaj +7 more sources
16S ribosomal RNA gene-based metagenomics: A review
Biomedical Research Journal, 2020 With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble +2 more
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Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies. [PDF]
J Anim Sci, 2022 Microbiome studies in animal science using 16S rRNA gene sequencing have become increasingly common in recent years as sequencing costs continue to fall and bioinformatic tools become more powerful and user-friendly. The combination of molecular biology,
Weinroth MD +16 more
europepmc +2 more sources
Shape changes and cooperativity in the folding of the central domain of the 16S ribosomal RNA. [PDF]
Proc Natl Acad Sci U S A, 2021 Significance Ribosomes are complexes between ribosomal RNA (rRNA) and a number of proteins. Because ribosome assembly begins with rRNA folding, we simulated the molecular details of Mg2+-driven folding of the central domain of the bacterial rRNA.
Hori N, Denesyuk NA, Thirumalai D.
europepmc +2 more sources
Diagnostic Value of 16S Ribosomal RNA Gene Polymerase Chain Reaction/Sanger Sequencing in Clinical Practice. [PDF]
Clin Infect Dis, 2021 BACKGROUND Accurate microbiologic diagnosis is important for appropriate management of infectious diseases. Sequencing-based molecular diagnostics are increasingly used for precision diagnosis of infections.
Fida M +8 more
europepmc +2 more sources
Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA [PDF]
Proceedings of the National Academy of Sciences, 1972 The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of individual proteins with RNA fragments purified from partial enzymatic digests. Of the six 30S subunit proteins known to interact directly with
Robert A. Zimmermann +4 more
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