Results 1 to 10 of about 129,309 (199)

16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences [PDF]

Frontiers in Bioinformatics, 2022
Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh, Yuan-Mao Hung, Mong-Hsun Tsai, Mong-Hsun Tsai, Liang-Chuan Lai, Liang-Chuan Lai, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang   +9 more
doaj   +2 more sources

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]

Frontiers in Cellular and Infection Microbiology, 2022
Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich, Vitor Heidrich, Lilian T. Inoue, Paula F. Asprino, Fabiana Bettoni, Antonio C. H. Mariotti, Diogo A. Bastos, Denis L. F. Jardim, Marco A. Arap, Anamaria A. Camargo   +9 more
doaj   +2 more sources

Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA [PDF]

Proceedings of the National Academy of Sciences of the United States of America, 1972
The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of individual proteins with RNA fragments purified from partial enzymatic digests. Of the six 30S subunit proteins known to interact directly with
Robert A Zimmermann, A Muto, P Fellner
exaly   +3 more sources

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]

PLoS ONE, 2015
Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt, Ozlem Buyuktanir, Janet Lindow, Elsio A Wunder, Mitermayer G Reis, Sahar Usmani-Brown, Michel Ledizet, Albert Ko, Utpal Pal   +8 more
doaj   +5 more sources

Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa [PDF]

Frontiers in Microbiology
IntroductionShort read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota.
Arunabh Sharma, Nadia Andrea Andreani, Nadia Andrea Andreani, Lara Keller, Lara Keller, Beate Herpertz-Dahlmann, Jochen Seitz, Jochen Seitz, John F. Baines, John F. Baines, Astrid Dempfle   +10 more
doaj   +2 more sources

Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]

Scientific Reports, 2021
We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish, Jonathan Shao, Lori L. Schreier, Monika Proszkowiec-Weglarz   +3 more
doaj   +2 more sources

Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene [PDF]

BMC Bioinformatics, 2018
Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo, Francesca Finotello, Enrico Lavezzo, Giacomo Baruzzo, Giulia Masi, Elektra Peta, Marco Falda, Stefano Toppo, Luisa Barzon, Barbara Di Camillo   +9 more
doaj   +2 more sources

A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]

Scientific Reports
Over half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan, Dina Alia, Mamoru Shiraishi, Megumi Higo, Yoshiaki Inoue, Koichi Hagiwara   +5 more
doaj   +2 more sources

A Mutant Era GTPase Suppresses Phenotypes Caused by Loss of Highly Conserved YbeY Protein in Escherichia coli

Frontiers in Microbiology, 2022
Ribosome assembly is a complex fundamental cellular process that involves assembling multiple ribosomal proteins and several ribosomal RNA species in a highly coordinated yet flexible and resilient manner.
Vignesh M. P. Babu, Siva Sankari, Anubrata Ghosal, Graham C. Walker   +3 more
doaj   +1 more source

Functional Modification of 16S Ribosomal RNA by Kethoxal [PDF]

Proceedings of the National Academy of Sciences, 1972
Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since ( a ) loss of specific binding of transfer RNA, but not binding of ...
H F, Noller, J B, Chaires
openaire   +2 more sources