Results 1 to 10 of about 186,676 (349)
Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich +9 more
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Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]
Scientific Reports, 2021 We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish +3 more
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Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene [PDF]
BMC Bioinformatics, 2018 Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo +9 more
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Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]
PLoS ONE, 2015 Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt +8 more
doaj +7 more sources
16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences [PDF]
Frontiers in Bioinformatics, 2022 Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh +9 more
doaj +2 more sources
Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA [PDF]
Proceedings of the National Academy of Sciences, 1972 The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of individual proteins with RNA fragments purified from partial enzymatic digests. Of the six 30S subunit proteins known to interact directly with
Robert A. Zimmermann +4 more
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Classification of methanogenic bacteria by 16S ribosomal RNA characterization [PDF]
Proceedings of the National Academy of Sciences, 1977 The 16S ribosomal RNAs from 10 species of methanogenic bacteria have been characterized in terms of the oligonucleotides produced by T 1 RNase digestion. Comparative analysis of these data reveals the methanogens to constitute a distinct phylogenetic group containing two major divisions. These organisms appear to be
Ralph S. Wolfe +4 more
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Chromosomal 16S Ribosomal RNA Methyltransferase RmtE1 in Escherichia coli Sequence Type 448 [PDF]
Emerging Infectious Diseases, 2017 We identified rmtE1, an uncommon 16S ribosomal methyltransferase gene, in an aminoglycoside- and cephalosporin-resistant Escherichia coli sequence type 448 clinical strain co-harboring blaCMY-2.
Bin Li, Marissa P. Pacey, Yohei Doi
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16S ribosomal RNA gene-based metagenomics: A review
Biomedical Research Journal, 2020 With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble +2 more
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Aptazyme-Mediated Regulation of 16S Ribosomal RNA
Chemistry & Biology, 2010 Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli.
Markus Wieland +3 more
openalex +5 more sources