16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences [PDF]
Frontiers in Bioinformatics, 2022 Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh +9 more
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Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich +9 more
doaj +3 more sources
PLoS ONE, 2019 The ribosome small subunit is expressed in all living cells. It performs numerous essential functions during translation, including formation of the initiation complex and proofreading of base-pairs between mRNA codons and tRNA anticodons.
Andrew M Smith +4 more
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Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]
Scientific Reports, 2021 We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish +3 more
doaj +3 more sources
Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]
PLoS ONE, 2015 Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt +8 more
doaj +6 more sources
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons [PDF]
Journal of Visualized Experiments, 2014 One of the major questions in microbial ecology is "who is there?" This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA.
Sylvie Sanschagrin, Étienne Yergeau
semanticscholar +4 more sources
Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies [PDF]
Nucleic Acids Research, 2012 16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
Anna Klindworth +6 more
openalex +2 more sources
Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies. [PDF]
J Anim Sci, 2022 Microbiome studies in animal science using 16S rRNA gene sequencing have become increasingly common in recent years as sequencing costs continue to fall and bioinformatic tools become more powerful and user-friendly. The combination of molecular biology,
Weinroth MD +16 more
europepmc +2 more sources
Shape changes and cooperativity in the folding of the central domain of the 16S ribosomal RNA. [PDF]
Proc Natl Acad Sci U S A, 2021 Significance Ribosomes are complexes between ribosomal RNA (rRNA) and a number of proteins. Because ribosome assembly begins with rRNA folding, we simulated the molecular details of Mg2+-driven folding of the central domain of the bacterial rRNA.
Hori N, Denesyuk NA, Thirumalai D.
europepmc +2 more sources
Diagnostic Value of 16S Ribosomal RNA Gene Polymerase Chain Reaction/Sanger Sequencing in Clinical Practice. [PDF]
Clin Infect Dis, 2021 BACKGROUND Accurate microbiologic diagnosis is important for appropriate management of infectious diseases. Sequencing-based molecular diagnostics are increasingly used for precision diagnosis of infections.
Fida M +8 more
europepmc +2 more sources