Results 1 to 10 of about 128,679 (180)

16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences [PDF]

open access: yesFrontiers in Bioinformatics, 2022
Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh   +9 more
doaj   +2 more sources

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich   +9 more
doaj   +2 more sources

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]

open access: yesPLoS ONE, 2015
Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt   +8 more
doaj   +5 more sources

16S ribosomal RNA gene-based metagenomics: A review

open access: yesBiomedical Research Journal, 2020
With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble   +2 more
doaj   +2 more sources

Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]

open access: yesScientific Reports, 2021
We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish   +3 more
doaj   +2 more sources

Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa [PDF]

open access: yesFrontiers in Microbiology
IntroductionShort read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota.
Arunabh Sharma   +10 more
doaj   +2 more sources

Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene [PDF]

open access: yesBMC Bioinformatics, 2018
Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo   +9 more
doaj   +4 more sources

A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]

open access: yesScientific Reports
Over half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan   +5 more
doaj   +2 more sources

Computer modeling 16 S ribosomal RNA [PDF]

open access: yesJournal of Molecular Biology, 1991
A three-dimensional structure for 16 S RNA has been produced with a computer protocol that is not dependent on human intervention. This protocol improves upon traditional modeling techniques by using distance geometry to fold the molecule in an objective and reproducible fashion.
J M, Hubbard, J E, Hearst
openaire   +2 more sources

A Mutant Era GTPase Suppresses Phenotypes Caused by Loss of Highly Conserved YbeY Protein in Escherichia coli

open access: yesFrontiers in Microbiology, 2022
Ribosome assembly is a complex fundamental cellular process that involves assembling multiple ribosomal proteins and several ribosomal RNA species in a highly coordinated yet flexible and resilient manner.
Vignesh M. P. Babu   +3 more
doaj   +1 more source

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