Reading canonical and modified nucleobases in 16S ribosomal RNA using nanopore native RNA sequencing. [PDF]
PLoS One, 2019 The ribosome small subunit is expressed in all living cells. It performs numerous essential functions during translation, including formation of the initiation complex and proofreading of base-pairs between mRNA codons and tRNA anticodons.
Smith AM +4 more
europepmc +4 more sources
Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling. [PDF]
Front Cell Infect Microbiol, 2022 Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Heidrich V +8 more
europepmc +4 more sources
16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences. [PDF]
Front Bioinform, 2022 Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Hsieh YP +4 more
europepmc +4 more sources
Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca. [PDF]
Sci Rep, 2021 We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Darwish N +3 more
europepmc +3 more sources
Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies [PDF]
Nucleic Acids Research, 2012 16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
Anna Klindworth +6 more
openalex +2 more sources
Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies. [PDF]
J Anim Sci, 2022 Microbiome studies in animal science using 16S rRNA gene sequencing have become increasingly common in recent years as sequencing costs continue to fall and bioinformatic tools become more powerful and user-friendly. The combination of molecular biology,
Weinroth MD +16 more
europepmc +2 more sources
Diagnostic Value of 16S Ribosomal RNA Gene Polymerase Chain Reaction/Sanger Sequencing in Clinical Practice. [PDF]
Clin Infect Dis, 2021 BACKGROUND Accurate microbiologic diagnosis is important for appropriate management of infectious diseases. Sequencing-based molecular diagnostics are increasingly used for precision diagnosis of infections.
Fida M +8 more
europepmc +2 more sources
Shape changes and cooperativity in the folding of the central domain of the 16S ribosomal RNA. [PDF]
Proc Natl Acad Sci U S A, 2021 Significance Ribosomes are complexes between ribosomal RNA (rRNA) and a number of proteins. Because ribosome assembly begins with rRNA folding, we simulated the molecular details of Mg2+-driven folding of the central domain of the bacterial rRNA.
Hori N, Denesyuk NA, Thirumalai D.
europepmc +2 more sources
16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis. [PDF]
Transl Vis Sci Technol, 2020 Purpose The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing bacterial keratitis. Methods Samples collected from 100 consecutive patients presenting to
Somerville TF +4 more
europepmc +2 more sources
Detection of Pathogenic Bacteria From Septic Patients Using 16S Ribosomal RNA Gene-Targeted Metagenomic Sequencing. [PDF]
Clin Infect Dis, 2021 Fida M +8 more
europepmc +3 more sources