16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences [PDF]
Frontiers in Bioinformatics, 2022 Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh +9 more
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Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich +9 more
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Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1972 The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of individual proteins with RNA fragments purified from partial enzymatic digests.
Christiane Branlant +2 more
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Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]
PLoS ONE, 2015 Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt +8 more
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Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]
Scientific Reports, 2021 We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish +3 more
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Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa [PDF]
Frontiers in MicrobiologyIntroductionShort read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota.
Arunabh Sharma +10 more
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Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene [PDF]
BMC Bioinformatics, 2018 Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo +9 more
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A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]
Scientific ReportsOver half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan +5 more
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The expanding role of 16s ribosomal RNA PCR in the management of patients with infective endocarditis undergoing cardiac surgery [PDF]
Frontiers in Cardiovascular MedicinePavone Natalia, Barbara Fiori
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Functional Modification of 16S Ribosomal RNA by Kethoxal [PDF]
Proceedings of the National Academy of Sciences, 1972 Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since ( a ) loss of specific binding of ...
H F, Noller, J B, Chaires
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