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Computer modeling 16S ribosomal RNA [PDF]
Journal of Molecular Biology, 1990 A three-dimensional structure for 16 S RNA has been produced with a computer protocol that is not dependent on human intervention. This protocol improves upon traditional modeling techniques by using distance geometry to fold the molecule in an objective and reproducible fashion.
John E. Hearst, John M. Hubbard
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A functional pseudoknot in 16S ribosomal RNA. [PDF]
The EMBO Journal, 1991 Several lines of evidence indicate that the universally conserved 530 loop of 16S ribosomal RNA plays a crucial role in translation, related to the binding of tRNA to the ribosomal A site. Based upon limited phylogenetic sequence variation, Woese and Gutell (1989) have proposed that residues 524-526 in the 530 hairpin loop are base paired with residues
Ted Powers, Harry F. Noller
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The structure of a methylated tetraloop in 16S ribosomal RNA [PDF]
Structure, 1998 Ribosomal RNAs contain many modified nucleotides. The functions of these nucleotides are poorly understood and few of them are strongly conserved. The final stem loop in 16S-like rRNAs is an exception in both regards. In both prokaryotes and eukaryotes, the tetranucleotide loop that caps the 3'-terminal stem contains two N6, N6-dimethyladenosine ...
Jason P. Rife, Peter B. Moore
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Functional Modification of 16S Ribosomal RNA by Kethoxal [PDF]
Proceedings of the National Academy of Sciences, 1972 Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since ( a ) loss of specific binding of transfer RNA, but not binding of ...
Jonathan B. Chaires, Harry F. Noller
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Role of 16‐S RNA in Ribosome Messenger Recognition [PDF]
European Journal of Biochemistry, 1980 The deoxyoctanucleotide (5′‐3′)d(A‐A‐G‐G‐A‐G‐G‐T), which is complementary to the 3′ end of 16‐S RNA, inhibits the formation of the complex between the 30‐S subunit and MS2 RNA described in the preceding paper. If the complex is preformed, the octanucleotide cannot prevent entry of the complex into the ribosome cycle upon supplementation with the ...
Jan van Duin +5 more
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Location of Ribosomal Protein Binding Sites on 16S Ribosomal RNA [PDF]
Proceedings of the National Academy of Sciences, 1972 The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of individual proteins with RNA fragments purified from partial enzymatic digests. Of the six 30S subunit proteins known to interact directly with
Chantal Ehresmann +4 more
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Aptazyme-Mediated Regulation of 16S Ribosomal RNA
Chemistry & Biology, 2010 Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli.
Wieland, Markus +3 more
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Mutations in 16S ribosomal RNA disrupt antibiotic-RNA interactions. [PDF]
The EMBO Journal, 1989 Two of six mutations at a base-paired site in Escherichia coli 16S rRNA confer resistance to nine different aminoglycoside antibiotics in vivo. Chemical probing of mutant and wild-type ribosomes in the presence of paromomycin indicates that interactions between the antibiotic and 16S rRNA in mutant ribosomes are disrupted.
Danesh Moazed +3 more
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Action spectra for UV-light induced RNA–RNA crosslinking in 16S ribosomal RNA in the ribosome [PDF]
Photochemical & Photobiological Sciences, 2003 UV irradiation induces intramolecular crosslinks in ribosomal RNA in the ribosome. These crosslinks occur between nucleotides distant in primary sequence and they are specific, limited in number and have crosslinking efficiencies sufficient to allow their use in monitoring conformational changes.
Oksana V. Zhirnov, Paul Wollenzien
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The 16S ribosomal RNA mutation database (16SMDB) [PDF]
Nucleic Acids Research, 1994 The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenotype has been detected by in vivo or ...
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