Results 1 to 10 of about 128,311 (135)

16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences [PDF]

Frontiers in Bioinformatics, 2022
Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh, Yuan-Mao Hung, Mong-Hsun Tsai, Mong-Hsun Tsai, Liang-Chuan Lai, Liang-Chuan Lai, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang   +9 more
doaj   +2 more sources

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]

Frontiers in Cellular and Infection Microbiology, 2022
Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich, Vitor Heidrich, Lilian T. Inoue, Paula F. Asprino, Fabiana Bettoni, Antonio C. H. Mariotti, Diogo A. Bastos, Denis L. F. Jardim, Marco A. Arap, Anamaria A. Camargo   +9 more
doaj   +2 more sources

Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa [PDF]

Frontiers in Microbiology
IntroductionShort read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota.
Arunabh Sharma, Nadia Andrea Andreani, Nadia Andrea Andreani, Lara Keller, Lara Keller, Beate Herpertz-Dahlmann, Jochen Seitz, Jochen Seitz, John F. Baines, John F. Baines, Astrid Dempfle   +10 more
doaj   +2 more sources

Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]

Scientific Reports, 2021
We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish, Jonathan Shao, Lori L. Schreier, Monika Proszkowiec-Weglarz   +3 more
doaj   +2 more sources

A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]

Scientific Reports
Over half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan, Dina Alia, Mamoru Shiraishi, Megumi Higo, Yoshiaki Inoue, Koichi Hagiwara   +5 more
doaj   +2 more sources

A Mutant Era GTPase Suppresses Phenotypes Caused by Loss of Highly Conserved YbeY Protein in Escherichia coli

Frontiers in Microbiology, 2022
Ribosome assembly is a complex fundamental cellular process that involves assembling multiple ribosomal proteins and several ribosomal RNA species in a highly coordinated yet flexible and resilient manner.
Vignesh M. P. Babu, Siva Sankari, Anubrata Ghosal, Graham C. Walker   +3 more
doaj   +1 more source

A comparison of structural and evolutionary attributes of Escherichia coli and Thermus thermophilus small ribosomal subunits: signatures of thermal adaptation. [PDF]

PLoS ONE, 2013
Here we compare the structural and evolutionary attributes of Thermus thermophilus and Escherichia coli small ribosomal subunits (SSU). Our results indicate that with few exceptions, thermophilic 16S ribosomal RNA (16S rRNA) is densely packed compared to
Saurav Mallik, Sudip Kundu
doaj   +1 more source

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]

PLoS ONE, 2015
Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt, Ozlem Buyuktanir, Janet Lindow, Elsio A Wunder, Mitermayer G Reis, Sahar Usmani-Brown, Michel Ledizet, Albert Ko, Utpal Pal   +8 more
doaj   +1 more source

16S ribosomal RNA gene-based metagenomics: A review

Biomedical Research Journal, 2020
With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble, Shriya Sawant, Harinder Singh   +2 more
doaj   +1 more source

Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae). [PDF]

PLoS ONE, 2014
To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin), elongation ...
Chunxiao Yang, Huipeng Pan, Yong Liu, Xuguo Zhou   +3 more
doaj   +1 more source