Results 11 to 20 of about 323,905 (300)
16S rRNA gene and 16S-23S rRNA gene internal transcribed spacer sequences analysis of the genus Myxococcus [PDF]
Phylogenetic relationships of the species belonging to the genus Myxococcus were elucidated based on the sequences of 16S rRNA genes and 16S-23S rRNA gene internal transcribed spacer (ITS) regions. The Myxococcus species were consequently classified into four distinct groups.
Mika Miyashita +3 more
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The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes +6 more
doaj +1 more source
Dumpster diving for diatom plastid 16S rRNA genes
High throughput sequencing is improving the efficiency of monitoring diatoms, which inhabit and support aquatic ecosystems across the globe. In this study, we explored the potential of a standard V4 515F-806RB primer pair in recovering diatom plastid 16S rRNA sequences.
Krista L. Bonfantine +4 more
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Reconstructing 16S rRNA genes in metagenomic data [PDF]
Abstract Metagenomic data, which contains sequenced DNA reads of uncultured microbial species from environmental samples, provide a unique opportunity to thoroughly analyze microbial species that have never been identified before. Reconstructing 16S ribosomal RNA, a phylogenetic marker gene, is usually required to analyze the composition
Yuan, Cheng +3 more
openaire +2 more sources
Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj +1 more source
Streamlined Method to Analyze 16S rRNA Gene Clone Libraries [PDF]
Kevin L. Vergin +2 more
doaj +3 more sources
Phylogenetic Clustering of Soil Microbial Communities by 16S rRNA but Not 16S rRNA Genes [PDF]
ABSTRACT We evaluated phylogenetic clustering of bacterial and archaeal communities from redox-dynamic subtropical forest soils that were defined by 16S rRNA and rRNA gene sequences. We observed significant clustering for the RNA-based communities but not the DNA-based communities, as well as increasing clustering over time of the highly ...
Kristen M, DeAngelis, Mary K, Firestone
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Species Identification and Profiling of Complex Microbial Communities Using Shotgun Illumina Sequencing of 16S rRNA Amplicon Sequences [PDF]
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Hibberd, Martin Lloyd +7 more
core +8 more sources
A renaissance for the pioneering 16S rRNA gene [PDF]
Culture-independent molecular surveys using the 16S rRNA gene have become a mainstay for characterizing microbial community structure over the past quarter century. More recently this approach has been overshadowed by metagenomics, which provides a global overview of a community's functional potential rather than just an inventory of its inhabitants ...
Tringe, SG, Hugenholtz, P
openaire +3 more sources
Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters
16S rRNA gene sequencing has engendered significant interest in studying microbial communities. There has been tension between trying to classify 16S rRNA gene sequences to increasingly lower taxonomic levels and the reality that those levels were ...
Patrick D. Schloss
doaj +1 more source

