Results 51 to 60 of about 323,905 (300)

Sequencing of 16S rRNA Gene: A Rapid Tool for Identification of Bacillus anthracis

open access: yesEmerging Infectious Diseases, 2002
In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA generom these ...
Claudio T. Sacchi   +7 more
doaj   +1 more source

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

open access: yesИнфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov   +6 more
doaj   +1 more source

Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing

open access: yesThe Journal of General and Applied Microbiology, 2015
In this study, the picocyanobacterial species composition of Lake Miyagase was examined by analyzing the 16S rRNA gene in a clone library and by amplicon sequencing using a benchtop next-generation sequencer. Five separate samples were analyzed from different days over a ten-month period. In the picocyanobacterial lineage, 9 and 12 OTUs were identified
Naoshi, Fujimoto   +10 more
openaire   +3 more sources

PCR primers to amplify 16S rRNA genes from cyanobacteria [PDF]

open access: yesApplied and Environmental Microbiology, 1997
We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaea. Gene segments selectively retrieved from cyanobacteria and diatoms
Nübel, U.   +2 more
openaire   +3 more sources

The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB

open access: yesInternational Journal of Mycobacteriology, 2012
Background: Conventional biochemical tests are the standard for the identification of Mycobacterium species, but molecular identifications are becoming more prevalent.
Yuko Kazumi, Satoshi Mitarai
doaj   +1 more source

Phylogenies of the 16S rRNA gene and its hypervariable regions lack concordance with core genome phylogenies

open access: yesMicrobiome, 2022
Background The 16S rRNA gene is used extensively in bacterial phylogenetics, in species delineation, and now widely in microbiome studies. However, the gene suffers from intragenomic heterogeneity, and reports of recombination and an unreliable ...
Hayley B. Hassler   +6 more
doaj   +1 more source

Two different 16S rRNA genes in a mycobacterial strain [PDF]

open access: yesJournal of Clinical Microbiology, 1996
Sequencing of the gene coding for 16S rRNA (16S rDNA) is a well-established method used to identify bacteria, particularly mycobacteria. Unique sequences allow identification of a particular genus and species. If more than one 16S rDNA is present on one mycobacterial genome, their sequences are assumed to be strictly or almost identical.
Bescher Ninet, Béatrice Alice   +7 more
openaire   +3 more sources

Nitrogen removal in a two-chambered microbial fuel cell: Establishment of a nitrifying-denitrifying microbial community on an intermittent aerated cathode [PDF]

open access: yes, 2016
A microbial fuel cell (MFC) was used to study nitrogen dynamics and its feasibility for high strength wastewater treatment. Intermittent aeration was applied on the cathode chamber accomplishing the establishment of a simultaneous nitrifying-denitrifying
Bonmatí Blasi, August   +3 more
core   +2 more sources

Genetic Environment of 16S rRNA Methylase Gene rmtD [PDF]

open access: yesAntimicrobial Agents and Chemotherapy, 2008
ABSTRACT The genetic environment of the 16S rRNA methylase gene rmtD was investigated. rmtD was flanked by a novel IS CR motif located downstream of class I integron In 163 in the original Pseudomonas aeruginosa
Doi, Y.   +2 more
openaire   +3 more sources

Taxonomic and Characterization Methods of Streptomyces: A Review

open access: yesProgress in Microbes and Molecular Biology, 2018
The 16S ribosomal RNA gene is the gold standard for taxonomic identification and phylogenetic study of most of the bacteria. However, the resolution of 16S rRNA gene is found to be insufficient to distinguish closely related Streptomyces species within ...
Jodi Woan-Fei Law   +4 more
doaj   +1 more source

Home - About - Disclaimer - Privacy