Results 61 to 70 of about 505,000 (387)

Diversity of 16S rRNA Genes within Individual Prokaryotic Genomes [PDF]

Applied and Environmental Microbiology, 2010
ABSTRACT Analysis of intragenomic variation of 16S rRNA genes is a unique approach to examining the concept of ribosomal constraints on rRNA genes; the degree of variation is an important parameter to consider for estimation of the diversity of a complex microbiome in the recently initiated Human Microbiome Project ...
Anna Y, Pei, William E, Oberdorf, Carlos W, Nossa, Ankush, Agarwal, Pooja, Chokshi, Erika A, Gerz, Zhida, Jin, Peng, Lee, Liying, Yang, Michael, Poles, Stuart M, Brown, Steven, Sotero, Todd, Desantis, Eoin, Brodie, Karen, Nelson, Zhiheng, Pei   +15 more
openaire   +3 more sources

Individual-specific changes in the human gut microbiota after challenge with enterotoxigenic Escherichia coli and subsequent ciprofloxacin treatment [PDF]

, 2016
Acknowledgements The authors wish to thank Mark Stares, Richard Rance, and other members of the Wellcome Trust Sanger Institute’s 454 sequencing team for generating the 16S rRNA gene data. Lili Fox Vélez provided editorial support.
Astrovskaya, Irina, Chakraborty, Subhra, Corrada Bravo, Héctor, Harro, Clayton, Li, Shan, Lindsay, Brianna R., Parkhill, Julian, Paulson, Joseph N., Pop, Mihai, Sack, David A., Stine, O. Colin, Walker, Alan W., Walker, Richard I.   +12 more
core   +2 more sources

Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

BMC Microbiology, 2020
Species-level genetic characterization of complex bacterial communities has important clinical applications in both diagnosis and treatment. Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene has proven to be a powerful strategy for the taxonomic ...
Y. Matsuo, S. Komiya, Y. Yasumizu, Yuki Yasuoka, K. Mizushima, T. Takagi, Kirill Kryukov, A. Fukuda, Y. Morimoto, Y. Naito, H. Okada, H. Bono, S. Nakagawa, K. Hirota   +13 more
semanticscholar   +1 more source

16S rRNA gene Library Preparation Protocol v1

, 2023
16S rRNA gene library preparation protocol including the following steps: MPure extraction of Bacterial DNA, Manual 16S indexed primer PCR, library clean up with beads, quibit DNA quantification, equimolar mix construction, agarose gel DNA concentration and gel clean up to produce a library ready for Illumina sequencing.
dinesh.aggarwal not provided, Katherine L Bellis, Josef Wagner, Ewan M. Harrison   +3 more
openaire   +1 more source

PCR-generated artefact from 16S rRNA gene-specific primers [PDF]

FEMS Microbiology Letters, 2005
Artefacts consisting of concatenated oligonucleotide primer sequences were generated during sub-optimally performing polymerase chain reaction amplification of bacterial 16S rRNA genes using a commonly employed primer pair. These artefacts were observed during amplification for terminal restriction fragment length polymorphism analyses of complex ...
Catherine A, Osborne, Maja, Galic, Parveen, Sangwan, Peter H, Janssen   +3 more
openaire   +2 more sources

MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons [PDF]

Frontiers in Cellular and Infection Microbiology, 2022
Human skin microbiome dysbiosis can have clinical consequences. Characterizing taxonomic composition of bacterial communities associated with skin disorders is important for dermatological advancement in both diagnosis and novel treatments. This study aims to analyze and improve the accuracy of taxonomic classification of skin bacteria with MinION ...
Miquel Rozas, Miquel Rozas, François Brillet, Chris Callewaert, Bernhard Paetzold   +4 more
openaire   +4 more sources

Sequencing of 16S rRNA Gene: A Rapid Tool for Identification of Bacillus anthracis

Emerging Infectious Diseases, 2002
In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA generom these ...
Claudio T. Sacchi, Anne M. Whitney, Leonard W. Mayer, Roger Morey, Arnold Steigerwalt, Ariana Boras, Robin S. Weyant, Tanja Popovic   +7 more
doaj   +1 more source

High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]

, 2012
The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Cogan, Tristan, Constantinidou, Chrystala, Pallen, Mark J., Penn, Charles W., Sergeant, Martin J.   +4 more
core   +3 more sources

Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory

Clinical Microbiology Reviews, 2020
This review provides a state-of-the-art description of the performance of Sanger cycle sequencing of the 16S rRNA gene for routine identification of bacteria in the clinical microbiology laboratory.
D. Church, L. Cerutti, Antoine Gürtler, T. Griener, A. Zelazny, S. Emler   +5 more
semanticscholar   +1 more source

Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing

The Journal of General and Applied Microbiology, 2015
In this study, the picocyanobacterial species composition of Lake Miyagase was examined by analyzing the 16S rRNA gene in a clone library and by amplicon sequencing using a benchtop next-generation sequencer. Five separate samples were analyzed from different days over a ten-month period. In the picocyanobacterial lineage, 9 and 12 OTUs were identified
Naoshi, Fujimoto, Keigo, Mizuno, Tomoki, Yokoyama, Akihiro, Ohnishi, Masaharu, Suzuki, Satoru, Watanabe, Kenji, Komatsu, Yoichi, Sakata, Naohiro, Kishida, Michihiro, Akiba, Satoko, Matsukura   +10 more
openaire   +3 more sources