Results 1 to 10 of about 245,723 (333)

Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system [PDF]

PeerJ, 2016
Over the past 10 years, microbial ecologists have largely abandoned sequencing 16S rRNA genes by the Sanger sequencing method and have instead adopted highly parallelized sequencing platforms.
Patrick D. Schloss, Matthew L. Jenior, Charles C. Koumpouras, Sarah L. Westcott, Sarah K. Highlander   +4 more
doaj   +5 more sources

Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]

PLoS ONE, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Swee Hoe Ong, Vinutha Uppoor Kukkillaya, Andreas Wilm, Christophe Lay, Eliza Xin Pei Ho, Louie Low, Martin Lloyd Hibberd, Niranjan Nagarajan   +7 more
doaj   +18 more sources

Research progress on the application of 16S rRNA gene sequencing and machine learning in forensic microbiome individual identification [PDF]

Frontiers in Microbiology
Forensic microbiome research is a field with a wide range of applications and a number of protocols have been developed for its use in this area of research. As individuals host radically different microbiota, the human microbiome is expected to become a
Mai-Qing Yang, Zheng-Jiang Wang, Chun-Bo Zhai, Li-Qian Chen   +3 more
doaj   +2 more sources

Gallstone and bile microbiota: A case-control study based on 16S rRNA gene sequencing. [PDF]

World J Gastrointest Surg
Lu ZX, Jiang YQ, Wang DS, Song YT, Jiang XM, Xu FJ, Tang J, Li B, Huang WH.   +8 more
europepmc   +2 more sources

16S rRNA gene sequencing reveals bacterial diversity in Khewra Salt Mine walls. [PDF]

Access Microbiol
Noor SW, Arshad S, Abdullah SM, Kayani BK, Fazal S.   +4 more
europepmc   +2 more sources

Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens

Microbiology Spectrum, 2022
16S rRNA gene sequencing is increasingly used in clinical practice for bacterial identification of clinical specimens. However, studies on its applicability to direct clinical specimens are limited. Here, we studied the diagnostic yield and impact of 16S
Pruke Eamsakulrat, Pitak Santanirand, Angsana Phuphuakrat   +2 more
doaj   +1 more source

16S-FASAS: an integrated pipeline for synthetic full-length 16S rRNA gene sequencing data analysis [PDF]

PeerJ, 2022
Background The full-length 16S rRNA sequencing can better improve the taxonomic and phylogenetic resolution compared to the partial 16S rRNA gene sequencing.
Ke Zhang, Rongnan Lin, Yujun Chang, Qing Zhou, Zhi Zhang   +4 more
doaj   +2 more sources

Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

Data in Brief, 2021
The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes, Jessica M. Ewald, Yi Liang, Andres Martinez, Andrew M. Awad, Keri C. Hornbuckle, Jerald L. Schnoor   +6 more
doaj   +1 more source

Performance of Conventional Urine Culture Compared to 16S rRNA Gene Amplicon Sequencing in Children with Suspected Urinary Tract Infection

Microbiology Spectrum, 2021
Because some organisms causing urinary tract infection (UTI) may be difficult to culture, examination of bacterial gene sequences in the urine may provide a more accurate view of bacteria present during a UTI.
Christopher W. Marshall, Marcia Kurs-Lasky, Christi L. McElheny, Sophia Bridwell, Hui Liu, Nader Shaikh   +5 more
doaj   +1 more source

Long-read MinION™ sequencing of 16S and 16S-ITS-23S rRNA genes provides species-level resolution of Lactobacillaceae in mixed communities

Frontiers in Microbiology, 2023
The Lactobacillaceae are lactic acid bacteria harnessed to deliver important outcomes across numerous industries, and their unambiguous, species-level identification from mixed community environments is an important endeavor.
Sandra A. Olivier, Sandra A. Olivier, Michelle K. Bull, Michelle K. Bull, Mikael Lenz Strube, Robert Murphy, Tom Ross, John P. Bowman, Belinda Chapman, Belinda Chapman   +9 more
doaj   +1 more source