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Research progress on the application of 16S rRNA gene sequencing and machine learning in forensic microbiome individual identification [PDF]

Frontiers in Microbiology
Forensic microbiome research is a field with a wide range of applications and a number of protocols have been developed for its use in this area of research. As individuals host radically different microbiota, the human microbiome is expected to become a
Mai-Qing Yang, Zheng-Jiang Wang, Chun-Bo Zhai, Li-Qian Chen   +3 more
doaj   +3 more sources

Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing

mSphere, 2021
In 16S rRNA gene sequencing, certain bacterial genera were found to be underrepresented or even missing in taxonomic profiles when using unsuitable primer combinations, outdated reference databases, or inadequate pipeline settings.
Isabel Abellan-Schneyder, Monica S. Matchado, Sandra Reitmeier, Alina Sommer, Zeno Sewald, Jan Baumbach, Markus List, Klaus Neuhaus   +7 more
doaj   +2 more sources

The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis

BMC Microbiology, 2021
Background 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host ...
M. Singer, R. Koedooder, M. P. Bos, L. Poort, S. Schoenmakers, P. H. M. Savelkoul, J. S. E. Laven, J. D. de Jonge, S. A. Morré, A. E. Budding   +9 more
doaj   +2 more sources

A preliminary study on the potential of Nanopore MinION and Illumina MiSeq 16S rRNA gene sequencing to characterize building-dust microbiomes [PDF]

Scientific Reports, 2020
There is a growing awareness of the importance of indoor microbiomes for human health. Given their complexity, these microbiomes can only be adequately surveyed using high throughput sequencing techniques.
Anders Benteson Nygaard, Hege Smith Tunsjø, Roger Meisal, Colin Charnock   +3 more
openalex   +2 more sources

Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples. [PDF]

BMC Genomics
Background Sequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study the composition of human microbiota.
Buetas E, Jordán-López M, López-Roldán A, D'Auria G, Martínez-Priego L, De Marco G, Carda-Diéguez M, Mira A.   +7 more
europepmc   +2 more sources

Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments

mSystems, 2019
Microbial communities are commonly studied using culture-independent methods, such as 16S rRNA gene sequencing. However, one challenge in accurately characterizing microbial communities is exogenous bacterial DNA contamination, particularly in low ...
Lisa Karstens, Mark Asquith, Sean Davin, Damien Fair, W. Thomas Gregory, Alan J. Wolfe, Jonathan Braun, Shannon McWeeney   +7 more
doaj   +2 more sources

Application of 16S rRNA gene sequencing in Helicobacter pylori detection [PDF]

PeerJ, 2020
Helicobacter pylori is one of the major stomach microbiome components, promoting development of inflammation and gastric cancer in humans. H. pylori has a unique ability to transform into a coccoidal form which is difficult to detect by many diagnostic ...
Aleksander Szymczak, Stanisław Ferenc, Joanna Majewska, Paulina Miernikiewicz, Jan Gnus, Wojciech Witkiewicz, Krystyna Dąbrowska   +6 more
doaj   +3 more sources

Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system [PDF]

PeerJ, 2016
Over the past 10 years, microbial ecologists have largely abandoned sequencing 16S rRNA genes by the Sanger sequencing method and have instead adopted highly parallelized sequencing platforms.
Patrick D. Schloss, Matthew L. Jenior, Charles C. Koumpouras, Sarah L. Westcott, Sarah K. Highlander   +4 more
doaj   +3 more sources

Gallstone and bile microbiota: A case-control study based on 16S rRNA gene sequencing. [PDF]

World J Gastrointest Surg
Lu ZX, Jiang YQ, Wang DS, Song YT, Jiang XM, Xu FJ, Tang J, Li B, Huang WH.   +8 more
europepmc   +2 more sources

16S rRNA gene sequencing reveals bacterial diversity in Khewra Salt Mine walls. [PDF]

Access Microbiol
Noor SW, Arshad S, Abdullah SM, Kayani BK, Fazal S.   +4 more
europepmc   +2 more sources