Results 131 to 140 of about 245,723 (333)

Minimizing spurious features in 16S rRNA gene amplicon sequencing

, 2018
The 16S rRNA gene amplicon sequencing is a widely used high-throughput method for the taxonomic inference in microbial communities. Many data analysis pipelines have been developed to enhance the accuracy in reflecting the real taxonomy, in order to better guide the downstream identification, isolation and mechanistic studies.
Wang, Jing, Zhang, Qianpeng, Wu, Guojun, Zhang, Chenhong, Zhang, Menghui, Zhao, Liping   +5 more
openaire   +1 more source

Unveiling Global Diversity of Patescibacteriota and Functional Interactions with Host Microbes

Advanced Science, EarlyView.
Patescibacteriota represents a diverse group of ultra‐small epibiotic bacteria, which is largely overlooked. By integrating ribosomal protein S3‐based community profiling with MAG‐based metabolic potential analyses, this study provides new insights into their distribution, diversity, and potential interactions with other bacteria across diverse ...
Yanhan Ji, Xu Liu, Shuai Zhao, Sihan Zhou, Yufan Yang, Ping Zhang, Yu Shi, Wei Qin, Guibing Zhu, Yongguan Zhu, Yanzheng Gao, Jiandong Jiang, Baozhan Wang   +12 more
wiley   +1 more source

Western Diet Inhibits FUT7‐Mediated Treg Intestinal Homing to Disrupt the Homeostasis of Intestinal Epithelial Cells in Crohn's Disease

Advanced Science, EarlyView.
In this study, we constructed a nanoparticle that was CD4 antibody‐mediated targeted, Fut7‐expressing plasmid‐loaded cationic liposome, namely CD4‐LDP‐Fut7. Upregulation of Fut7 expression in Tregs by using CD4‐LDP‐Fut7 can increase Treg homing to the intestine, thereby facilitating repair of the intestinal epithelial barrier and inhibiting ...
Qian Zhou, Xianfei Wang, Teng Ben, Chunping Zhu, Xinlong Lin, Yinmu Li, Xinyue Zhang, Yeling Chen, Yuchen Xuan, Huaiwen Chen, Ke Liu, Fang Wu, Yuexin Ren, Gao Tan   +13 more
wiley   +1 more source

Cinnamic‐Hydroxamic‐Acid Derivatives Exhibit Antibiotic, Anti‐Biofilm, and Supercoiling Relaxation Properties by Targeting Bacterial Nucleoid‐Associated Protein HU

Advanced Science, EarlyView.
Cinnamic‐hydroxamic‐acid derivatives (CHADs) are identified as novel inhibitors of the bacterial nucleoid‐associated protein HU, exhibiting potent antibacterial, anti‐biofilm (both inhibition and eradication), and DNA relaxation (anti‐supercoiling) activities. Moreover, CHADs demonstrate strong synergistic effects with multiple antibiotics.
Huan Chen, Yingqi Xu, Zhaoping Xiong, Hongliang Wang, Xin Wang, Yue Kang, Zhonglin Wang, Xiaoyan Zeng, Yahui Liu, Yehuan Zheng, Wei Chen, Mengzhe Li, Zhenyue Hu, Chi Xu, Yue Wu, Yawen Wang, Zuyi Yuan, Shuai Yuan, Huadong Liu, Steve Matthews, Nan Qiao, Yan Li, Bing Liu   +22 more
wiley   +1 more source

Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences [PDF]

, 1993
M S Hughes, Robin Skuce, L A Beck, S.D. Neill   +3 more
openalex   +1 more source

Lactylation‐Driven YTHDC1 Alleviates MASLD by Suppressing PTPN22‐Mediated Dephosphorylation of NLRP3

Advanced Science, EarlyView.
In MASLD, YTHDC1 undergoes increased lactylation and ubiquitination, reducing its expression. AARS1 mediates lactylation at lysine 565, while disrupted binding to LDHA further promotes lactylation, suppressing YTHDC1. This downregulation enhances PTPN22 mRNA stability, leading to NLRP3 dephosphorylation and activation, which exacerbates inflammation ...
Feng Zhang, Linghua Zeng, Kunkun Zou, Keying Lin, Fangting Yao, Jinglun Song, Shumeng Zhang, Hanshu Feng, Zhichao Yang, Chunlei Wang, Hongtao Diao, Xue Kong, Tengfei Pan, Joonki Kim, Tianqi Duo, Linqiang Li, Yu Bian   +16 more
wiley   +1 more source

Automated Identification of Medically Important Bacteria by 16S rRNA Gene Sequencing Using a Novel Comprehensive Database, 16SpathDB [PDF]

, 2011
Patrick C. Y. Woo, Jade L. L. Teng, Juilian M. Y. Yeung, Herman Tse, Susanna K. P. Lau, Kwok‐Yung Yuen   +5 more
openalex   +1 more source

Two intracellular and cell type-specific bacterial symbionts in the placozoan Trichoplax H2

, 2019
Placozoa is an enigmatic phylum of simple, microscopic, marine metazoans(1,2). Although intracellular bacteria have been found in all members of this phylum, almost nothing is known about their identity, location and interactions with their host(3-6). We
Dubilier, N., Gruber-Vodicka, H., Hadfield, M., Hinzke, T., Kleiner, M., Leisch, N., Liebeke, M., McFall-Ngai, M.   +7 more
core   +1 more source

Alkyltriphenylphosphonium Binding to Cardiolipin Triggers Oncosis in Cancer Cells

Advanced Science, EarlyView.
Alkyltriphenylphosphonium, exemplified by TPP+‐C14, preferentially accumulates in mitochondria and selectively binds to cardiolipin, a key phospholipid of the inner mitochondrial membrane, causing loss of mitochondrial membrane potential, severe cellular ATP depletion, and calcium imbalance.
Jin Li, Hang Zheng, Yuxing Lin, Ziwei Zhao, Yijia Mai, Zhangrong Lou, Qiang Liu, Zhao Ma, Chengjun Wu   +8 more
wiley   +1 more source

Short-read full-length 16S rRNA amplicon sequencing for characterisation of the respiratory bacteriome of captive and free-ranging African elephants (Loxodonta africana)

Scientific Reports
Hypervariable region sequencing of the 16S ribosomal RNA (rRNA) gene plays a critical role in microbial ecology by offering insights into bacterial communities within specific niches. While providing valuable genus-level information, its reliance on data
Lauren C. Martin, Michaela A. O’Hare, Giovanni Ghielmetti, David Twesigomwe, Tanya J. Kerr, Rachiel Gumbo, Peter E. Buss, Natasha Kitchin, Sian M. J. Hemmings, Michele A. Miller, Wynand J. Goosen   +10 more
doaj   +1 more source