Results 21 to 30 of about 253,664 (304)

Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

BMC Microbiology, 2021
Background Species-level genetic characterization of complex bacterial communities has important clinical applications in both diagnosis and treatment. Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene has proven to be a powerful strategy for the ...
Yoshiyuki Matsuo, Shinnosuke Komiya, Yoshiaki Yasumizu, Yuki Yasuoka, Katsura Mizushima, Tomohisa Takagi, Kirill Kryukov, Aisaku Fukuda, Yoshiharu Morimoto, Yuji Naito, Hidetaka Okada, Hidemasa Bono, So Nakagawa, Kiichi Hirota   +13 more
doaj   +1 more source

16S classifier: a tool for fast and accurate taxonomic classification of 16S rRNA hypervariable regions in metagenomic datasets. [PDF]

PLoS ONE, 2015
The diversity of microbial species in a metagenomic study is commonly assessed using 16S rRNA gene sequencing. With the rapid developments in genome sequencing technologies, the focus has shifted towards the sequencing of hypervariable regions of 16S ...
Nikhil Chaudhary, Ashok K Sharma, Piyush Agarwal, Ankit Gupta, Vineet K Sharma   +4 more
doaj   +1 more source

Quantifying dominant bacterial genera detected in metagenomic data from fish eggs and larvae using genus‐specific primers

MicrobiologyOpen, 2022
The goal of this study was to design genus‐specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon‐based sequencing of the 16S rRNA gene in fish‐related samples and surrounding water. Efficient genus‐specific
Babak Najafpour, Patricia I. S. Pinto, A. V. M. Canario, Deborah M. Power   +3 more
doaj   +1 more source

Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters

mSphere, 2021
16S rRNA gene sequencing has engendered significant interest in studying microbial communities. There has been tension between trying to classify 16S rRNA gene sequences to increasingly lower taxonomic levels and the reality that those levels were ...
Patrick D. Schloss
doaj   +1 more source

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

Инфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov, S. N. Zhdanova, E. A. Orlova, P. A. Khromova, N. L. Belkova, V. V. Sinkov, I. G. Kondratov   +6 more
doaj   +1 more source

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling

Frontiers in Cellular and Infection Microbiology, 2022
Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich, Vitor Heidrich, Lilian T. Inoue, Paula F. Asprino, Fabiana Bettoni, Antonio C. H. Mariotti, Diogo A. Bastos, Denis L. F. Jardim, Marco A. Arap, Anamaria A. Camargo   +9 more
doaj   +1 more source

Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis

Microbiome, 2014
Experimental designs that take advantage of high-throughput sequencing to generate datasets include RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), sequencing of 16S rRNA gene fragments, metagenomic analysis and selective ...
A. Fernandes, J. N. Reid, Jean M. Macklaim, T. A. McMurrough, D. Edgell, G. Gloor   +5 more
semanticscholar   +1 more source

Progress in the 16S rRNA Gene Sequencing in Forensic Science [PDF]

Fayixue Zazhi, 2018
Forensic microorganism is one of the hotspots of forensic science research. Due to its conservatism and specificity, the 16S rRNA gene is found to be an ideal marker for forensic identification.
SONG Guo-qing, CAO Yu, LI Hui，et al.
doaj   +1 more source

Concatenated 16S rRNA sequence analysis improves bacterial taxonomy [version 3; peer review: 2 approved]

F1000Research, 2023
Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj   +1 more source

Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis

Nature Communications, 2019
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for decades. However, high-throughput sequencing of the full gene has only recently become a realistic prospect.
Jethro S. Johnson, Daniel J. Spakowicz, B. Hong, Lauren M. Petersen, Patrick C. Demkowicz, Lei Chen, Shana R. Leopold, B. Hanson, Hanako Agresta, M. Gerstein, E. Sodergren, G. Weinstock   +11 more
semanticscholar   +1 more source