Results 41 to 50 of about 1,076,866 (305)

16S rRNA Amplicon Sequencing for Epidemiological Surveys of Bacteria in Wildlife [PDF]

mSystems, 2016
Several recent public health crises have shown that the surveillance of zoonotic agents in wildlife is important to prevent pandemic risks. High-throughput sequencing (HTS) technologies are potentially useful for this surveillance, but rigorous experimental processes are required for the use of these effective tools in such epidemiological contexts. In
Galan, Maxime, Razzauti, Maria, Bard, Emilie, Bernard, Maria, Brouat, Carine, Charbonnel, Nathalie, Dehne-Garcia, Alexandre, Loiseau, Anne, Tatard, Caroline, Tamisier, Lucie, Vayssier-Taussat, Muriel, Vignes, Hélène, Cosson, Jean-François   +12 more
openaire   +7 more sources

Comparison of bacterial culture and 16S rRNA community profiling by clonal analysis and pyrosequencing for the characterization of the dentine caries-associated microbiome [PDF]

, 2014
Culture-independent analyses have greatly expanded knowledge regarding the composition of complex bacterial communities including those associated with oral diseases.
Kathrin eSchulze-Schweifing, Avijit eBanerjee, Wade, William G, Banerjee, A, Schulze-Schweifing, K, Schulze-Schweifing, Kathrin, Wade, WG, Banerjee, Avijit; id_orcid, William Geoffrey Wade, William G. Wade, Kathrin Schulze-Schweifing, Avijit Banerjee   +11 more
core   +1 more source

Sequence Diversity of Neisseria meningitidis 16S rRNA Genes and Use of 16S rRNA Gene Sequencing as a Molecular Subtyping Tool [PDF]

Journal of Clinical Microbiology, 2002
ABSTRACT We investigated the diversity of the primary sequences of 16S rRNA genes among Neisseria meningitidis strains (Men) and evaluated the use of this approach as a molecular subtyping tool. We aligned and compared a 1,417-bp fragment of the 16S rRNA gene from 264 Men strains of serogroups A, B, C, and
Claudio T, Sacchi, Anne M, Whitney, Michael W, Reeves, Leonard W, Mayer, Tanja, Popovic   +4 more
openaire   +2 more sources

Development and Validation of a Reference Data Set for Assigning Staphylococcus Species Based on Next-Generation Sequencing of the 16S-23S rRNA Region

Frontiers in Cellular and Infection Microbiology, 2019
Many members of the Staphylococcus genus are clinically relevant opportunistic pathogens that warrant accurate and rapid identification for targeted therapy.
Maja Kosecka-Strojek, Maja Kosecka-Strojek, Artur J. Sabat, Viktoria Akkerboom, Karsten Becker, Evert van Zanten, Guido Wisselink, Jacek Miedzobrodzki, Anna M. D. (Mirjam) Kooistra-Smid, Anna M. D. (Mirjam) Kooistra-Smid, Alexander W. Friedrich   +10 more
doaj   +1 more source

Quantifying dominant bacterial genera detected in metagenomic data from fish eggs and larvae using genus‐specific primers

MicrobiologyOpen, 2022
The goal of this study was to design genus‐specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon‐based sequencing of the 16S rRNA gene in fish‐related samples and surrounding water. Efficient genus‐specific
Babak Najafpour, Patricia I. S. Pinto, A. V. M. Canario, Deborah M. Power   +3 more
doaj   +1 more source

Towards a Phylogeny of the Genus Vibrio Based on 16S rRNA Sequences [PDF]

International Journal of Systematic Bacteriology, 1992
The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae.
Dorsch M., Lane D., Stackebrandt E.
openaire   +4 more sources

Molecular biological methods for studying the gut microbiota : the EU human gut flora project [PDF]

, 2002
Seven European laboratories co-operated in a joint project (FAIR CT97-3035) to develop, refine and apply molecular methods towards facilitating elucidation of the complex composition of the human intestinal microflora and to devise robust methodologies ...
Vos, W.M., de, Collins, M.D., Welling, G.W., Loo, J., van, Blaut, M., Dore, J., Welling, G. W., Collins, M. D., de Vos, W., W. de Vos, M.D. Collins, J. Doré, van Loo, J., G. W. Welling, J. van Loo, M. Blaut   +15 more
core   +1 more source

Results 16s rRNA gene amplification and sequencing.

, 2022
This table contains the 16s rRNA gene amplification and sequencing results presented in Table 5 of the manuscript. (XLSX)
Nicole Wente (13014753), Yanchao Zhang (4905979), Volker Krömker (13014756), Anneke Lücken (13014750), Svenja Woudstra (4843422)   +4 more
core   +1 more source

Computational methods for 16S metabarcoding studies using Nanopore sequencing data

Computational and Structural Biotechnology Journal, 2020
Assessment of bacterial diversity through sequencing of 16S ribosomal RNA (16S rRNA) genes has been an approach widely used in environmental microbiology, particularly since the advent of high-throughput sequencing technologies.
Andres Santos, Ronny van Aerle, Leticia Barrientos, Jaime Martinez-Urtaza   +3 more
doaj   +1 more source

Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine [PDF]

, 2009
BACKGROUND: Variations in the composition of the human intestinal microbiota are linked to diverse health conditions. High-throughput molecular technologies have recently elucidated microbial community structure at much higher resolution than was ...
Vos, W.M., de, Willem M de Vos, O\u27Toole, Paul W., Smidt, H., O'Sullivan, O., Janne Nikkilä, Claesson Marcus J., de Vos Willem M., Hauke Smidt, O'Sullivan, Orla, Marchesi, Julian Roberto, O\u27Sullivan, Orla, Ross R. Paul, Smidt, Hauka, Ross, R. Paul, Ross, R. P., Paul W O'Toole, Wang, Qiong, Orla O'Sullivan, O'Sullivan Orla, Qiong Wang, Nikkilä Janne, O'Toole, Paul W., Claesson, M.J., Marchesi, J.R., O'Toole, P.W., Claesson, Marcus J., O'Toole, P. W., Wang, Q., de Vos, Willem M., Claesson, M. J., Julian R Marchesi, O'Toole Paul W., Marchesi, Julian R., Smidt, Hauke, Ross, R.P., Smidt Hauke, Nikkila, Janne, R Paul Ross, Marchesi Julian R., Ross, R Paul, Wang Qiong, De Vos, W. M., Nikkilä, J., Marcus J Claesson, O'Sullivan, Orla, O'Toole, Paul W.   +46 more
core   +1 more source