Results 61 to 70 of about 1,076,866 (305)

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

Bacterial identification in clinical samples – comparing Nanopore and Illumina sequencing of the 16S rRNA and rpoB genes

BMC Infectious Diseases
Background Recent improvements in the sequencing accuracy of the Oxford Nanopore Technologies (ONT) platform have increased its suitability for clinical microbiology applications, including broad-range amplification and sequencing of the 16S ribosomal ...
Joanna Małgorzata Bivand, Audun Sivertsen, Marit Gjerde Tellevik, Oystein Saebo, Robin Patel, Ruben Dyrhovden, Øyvind Kommedal   +6 more
doaj   +1 more source

metaSPARSim: a 16S rRNA gene sequencing count data simulator

BMC Bioinformatics, 2019
Background In the last few years, 16S rRNA gene sequencing (16S rDNA-seq) has seen a surprisingly rapid increase in election rate as a methodology to perform microbial community studies.
Ilaria Patuzzi, Giacomo Baruzzo, Carmen Losasso, Antonia Ricci, Barbara Di Camillo   +4 more
doaj   +1 more source

Microbial Identification Using rRNA Operon Region: Database and Tool for Metataxonomics with Long-Read Sequence

Microbiology Spectrum, 2022
Recent development of long-read sequencing platforms has enabled researchers to explore bacterial community structure through analysis of full-length 16S rRNA gene (∼1,500 bp) or 16S-ITS-23S rRNA operon region (∼4,300 bp), resulting in higher taxonomic ...
Donghyeok Seol, Jin Soo Lim, Samsun Sung, Young Ho Lee, Misun Jeong, Seoae Cho, Woori Kwak, Heebal Kim   +7 more
doaj   +1 more source

SPHINX31 acts as a SRPK1 inhibitor targeting the ATR/DNA‐PKcs/CHK1 replicative checkpoint to inhibit cell growth in non‐small cell lung cancer

Molecular Oncology, EarlyView.
The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim, Aurelie Genoux, Nadiia Zubchuk, Haoyang Zheng, Perla Salameh, Theo Ziegelmeyer, Fabien Dalonneau, Margot Rouchette, Christiane Oddou, Simona Miron, Helene Polveche, Adiilah Mamode Cassim, Carmen Garrido, Eleni Nikolakaki, Didier Auboeuf, Tao Jia, Sophie Zinn‐Justin, Beatrice Eymin   +17 more
wiley   +1 more source

16S rRNA and metagenomic shotgun sequencing data revealed consistent patterns of gut microbiome signature in pediatric ulcerative colitis

Scientific Reports, 2022
Dysbiosis of human gut microbiota has been reported in association with ulcerative colitis (UC) in both children and adults using either 16S rRNA gene or shotgun sequencing data.
Wenxuan Zuo, Beibei Wang, Xin Bai, Yihui Luan, Yingying Fan, Sonia Michail, Fengzhu Sun   +6 more
doaj   +1 more source

The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection

Molecular Oncology, EarlyView.
Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński, Krzysztof Pastuszak, Anna Samelak‐Czajka, Zuzanna Cemka, Paulina Jackowiak, Tomasz Stefaniak, Sylwia Łapińska‐Szumczyk, Jacek Jassem, Anna J. Żaczek, Matthew T. Rondina, Myron G. Best, Sjors G. J. G. In 't Veld, Anna Supernat   +12 more
wiley   +1 more source

Circulating microbiome profiling in transjugular intrahepatic portosystemic shunt patients: 16S rRNA vs. shotgun sequencing

Frontiers in Medicine
Background and aimCurrent efforts to characterize the circulating microbiome are constrained by the lack of standardized protocols for isolating and sequencing microbial communities in blood.
Jingxiang Zhang, Xiaoshuang Xu, Lei Chen, Xincheng Yang, Júlio Ken Matsubara, Yuyao Tian, Jiahua Liu, Xiaoxu Jin, He Chang, Menghui Xu, Chunyang Zhu, Xin Wang, Lingxuan Ren, Jiale Xie, Jiale Liu, Guifen Liu, Mengyao Lu, Xiaowen Wang, Longqi Du, Zilin Ma, Xuechen Liu, Huimin Zhao, Wei Chen, Xiaohui Huo, Guoqi Zheng, Changshun Xie, Chao Xu, Xueqiang Zhang, Wei Qi, Zhijie Feng   +29 more
doaj   +1 more source

MinION Sequencing of colorectal cancer tumour microbiomes-A comparison with amplicon-based and RNA-Sequencing.

PLoS ONE, 2020
BACKGROUND:Recent evidence suggests a role for the gut microbiome in the development and progression of many diseases and many studies have been carried out to analyse the microbiome using a variety of methods. In this study, we compare MinION sequencing
William S Taylor, John Pearson, Allison Miller, Sebastian Schmeier, Frank A Frizelle, Rachel V Purcell   +5 more
doaj   +1 more source

Taxonomy of the canine Mollicutes by 16S rRNA gene and 16S/23S rRNA intergenic spacer region sequence comparison [PDF]

International Journal of Systematic and Evolutionary Microbiology, 2004
The taxonomy of canine Mollicutes is described, based on phylogenetic analysis of 16S rRNA gene and 16S/23S rRNA intergenic spacer (IGS) region sequences. The nucleotide sequences of the 16S rRNA gene of two untyped mycoplasmas and the IGS region of 11 Mycoplasma species were determined and used for phylogenetic analysis.
Victoria J, Chalker, Joe, Brownlie
openaire   +2 more sources