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Enhanced 3-Epi-25-Hydroxyvitamin D3 Signal Leads to Overestimation of Its Concentration and Amplifies Interference in 25-Hydroxyvitamin D LC-MS/MS Assays [PDF]
Annals of Clinical Biochemistry, 2014 Background 3-epi-25-hydroxyvitamin D3 (3-epi-25OHD3) interferes in most liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays for 25-hydroxyvitamin D (25OHD).
Lam, F, Flynn, N, Dawnay, A
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Hepatic accumulation of vitamin D3 and 25-hydroxyvitamin D3☆
Biochimica et Biophysica Acta (BBA) - General Subjects, 1976Concomitant intravenous administration of 25-hydroxycholecalciferol and [3H] vitamin D3 to vitamin D-depleted rats did not affect the conversion of [3H] vitamin D3 to 25-OH-[3H] vitamin D3 as indicated by a serum 25-OH-[3H] vitamin D3 to content at 3 and 24 h identical to those observed in animals receiving [3H] vitamin D3 alone.
S, Rojanasathit, J G, Haddad
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Archives of Biochemistry and Biophysics, 1978
Abstract An antibody was prepared from serum of rabbits injected with a pure inhibitor protein obtained from rat serum for chick renal 25-hydroxyvitamin D 3 -1α-hydroxylase. The antibody was separated from the endogenous inhibitor in rabbit serum.
J G, Ghazarian +4 more
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Abstract An antibody was prepared from serum of rabbits injected with a pure inhibitor protein obtained from rat serum for chick renal 25-hydroxyvitamin D 3 -1α-hydroxylase. The antibody was separated from the endogenous inhibitor in rabbit serum.
J G, Ghazarian +4 more
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Biotransformations of 25-hydroxyvitamin D3 by kidney microsomes
Biochemical and Biophysical Research Communications, 1978Abstract Vitamin D3-deficient chick kidney microsomes in vitro metabolize 25-hydroxy-[26(27)-methyl-3H]-vitamin D3 to yet structurally unidentified polar metabolites previously designated MIC-I and MIC-II. Kidney microsomes of vitamin D3-repleted chicks could not be demonstrated to produce these metabolites when 3H was the radioactive isotope ...
R W, Gray +3 more
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Kidney microsomal metabolism of 25-hydroxyvitamin D3
Biochemical and Biophysical Research Communications, 1977Abstract Vitamin D3-deficient chick kidney microsomes in vitro metabolize 25-hydroxyvitamin D3 to two polar metabolites by a pathway which may involve side-chain modification. Molecular oxygen and a source of reduced nicotinamide adenine dinucleotide phosphate are required for this metabolism.
J G, Ghazarian, H F, Deluca
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Renal Actions of 25-Hydroxyvitamin D3
1977The kidney is uniquely involved in the maintenance of body calcium, magnesium, phosphorus, and acid-base balance, both as an endocrine secretory and a target organ. In this light, the kidney reabsorbs calcium and phosphorus more avidly under the stimulus of 25-hydroxycholecalciferol (1–4) while it effects conversion of this compound to the metabolite 1,
R A, Peraino +3 more
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3-Epimer-25-hydroxyvitamin D3 interference in assays for the measurement of 25-hydroxyvitamin D3
The Journal of Steroid Biochemistry and Molecular BiologyIn chromatographic measurements of vitamin D, such as liquid chromatography tandem mass spectrometry (LC-MS/MS), 3-epi-25hydroxyvitamin D3 (3-epi-25OHD3) can interfere with 25-hydroxyvitamin D3 (25OHD3) quantification due to their identical mass. Chromatographic separation is therefore required to overcome this issue.
Alex N. Shaw, Emma L. Williams
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Biochemistry, 1981
Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions, the metabolites were
J K, Wichmann, H K, Schnoes, H F, DeLuca
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Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions, the metabolites were
J K, Wichmann, H K, Schnoes, H F, DeLuca
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Analytical Biochemistry, 1983
Extraction, lipid-reduction, and chromatographic methods suitable for the resolution and subsequent quantitation of vitamin D2, vitamin D3, 25-hydroxyvitamin D2, and 25-hydroxy-vitamin D3 from human milk are described. This procedure utilizes a methanol:methylene chloride extraction, precipitation of unwanted lipids with cold methanol and ether ...
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Extraction, lipid-reduction, and chromatographic methods suitable for the resolution and subsequent quantitation of vitamin D2, vitamin D3, 25-hydroxyvitamin D2, and 25-hydroxy-vitamin D3 from human milk are described. This procedure utilizes a methanol:methylene chloride extraction, precipitation of unwanted lipids with cold methanol and ether ...
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The Effect of 25-Hydroxyvitamin D3 on the Immune System
Journal of Pediatric Endocrinology and Metabolism, 2009To examine the effect of 25-OH vitamin D3 on the immune system in patients with nutritional rickets.Fifty-three patients were included in our study between April 2002 and March 2004. Diagnosis of rickets was based on clinical, biochemical and radiological examinations.
Erol, Mehmet +4 more
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