Regulation of repair by the 26S proteasome [PDF]
BioMed Research International, 2002 Cellular processes such as transcription and DNA repair may be regulated through diverse mechanisms, including RNA synthesis, protein synthesis, posttranslational modification and protein degradation. The 26S proteasome, which is responsible for degrading a broad spectrum of proteins, has been shown to interact with several nucleotide excision repair ...
Sweder, K., Madura, K.
openaire +2 more sources
The regulatory complex of Drosophila melanogaster 26S proteasomes : subunit composition and localization of a deubiquitylating enzyme [PDF]
, 2000 Drosophila melanogaster embryos are a source for homogeneous and stable 26S proteasomes suitable for structural studies. For biochemical characterization, purified 26S proteasomes were resolved by two- dimensional (2D) gel electrophoresis and subunits ...
Harald Hölzl +22 more
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Protein Degradation of RNA Polymerase II-Association Factor 1(PAF1) Is Controlled by CNOT4 and 26S Proteasome. [PDF]
PLoS ONE, 2015 The PAF complex (PAFc) participates in various steps of the transcriptional process, from initiation to termination, by interacting with and recruiting various proteins to the proper locus for each step. PAFc is an evolutionarily conserved, multi-protein
Hwa-Young Sun +3 more
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Wiggle and Shake: Managing and Exploiting Conformational Dynamics during Proteasome Biogenesis
Biomolecules, 2023 The 26S proteasome is the largest and most complicated protease known, and changes to proteasome assembly or function contribute to numerous human diseases.
Daniel Betancourt +2 more
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Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements [PDF]
, 2002 The subunit contacts in the regulatory complex of the Drosophila 26 S proteasome were studied through the cross-linking of closely spaced subunits of the complex, and analysis of the cross-linking pattern in an immunoblot assay with the use of subunit ...
Kapelari, Barbara +13 more
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Frontiers in Chemistry, 2018 The proteasome is a validated target in drug discovery for diseases associated with unusual proteasomal activity. Here we report that two diphenyldihaloketones, CLEFMA and EF24, inhibit the peptidase activity of the 26S proteasome.
Geeta Rao +6 more
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Poly-Ub-substrate-degradative activity of 26S proteasome is not impaired in the aging rat brain. [PDF]
PLoS ONE, 2013 Proteostasis is critical for the maintenance of life. In neuronal cells an imbalance between protein synthesis and degradation is thought to be involved in the pathogenesis of neurodegenerative diseases during aging.
Carolin Giannini +7 more
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The proteasome cap RPT5/Rpt5p subunit prevents aggregation of unfolded ricin A chain [PDF]
, 2013 The plant cytotoxin ricin enters mammalian cells by receptor-mediated endocytosis, undergoing retrograde transport to the endoplasmic reticulum (ER) where its catalytic A chain (RTA) is reductively separated from the holotoxin to enter the cytosol and ...
Spooner, Robert A. +12 more
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Proteasome Activity Is Affected by Fluctuations in Insulin-Degrading Enzyme Distribution.
PLoS ONE, 2015 Insulin-Degrading-Enzyme (IDE) is a Zn2+-dependent peptidase highly conserved throughout evolution and ubiquitously distributed in mammalian tissues wherein it displays a prevalent cytosolic localization.
Diego Sbardella +9 more
doaj +1 more source
Early cysteine-dependent inactivation of 26S proteasomes does not involve particle disassembly
Redox Biology, 2018 Under oxidative stress 26S proteasomes suffer reversible disassembly into its 20S and 19S subunits, a process mediated by HSP70. This inhibits the degradation of polyubiquitinated proteins by the 26S proteasome and allows the degradation of oxidized ...
Martín Hugo +7 more
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