Systematic identification of 20S proteasome substrates [PDF]
Molecular Systems BiologyFor years, proteasomal degradation was predominantly attributed to the ubiquitin-26S proteasome pathway. However, it is now evident that the core 20S proteasome can independently target proteins for degradation.
Monika Pepelnjak +7 more
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The Contribution of the 20S Proteasome to Proteostasis [PDF]
Biomolecules, 2019 The last decade has seen accumulating evidence of various proteins being degraded by the core 20S proteasome, without its regulatory particle(s). Here, we will describe recent advances in our knowledge of the functional aspects of the 20S proteasome ...
Fanindra Kumar Deshmukh +4 more
doaj +5 more sources
Nostocyclopeptides as New Inhibitors of 20S Proteasome. [PDF]
Biomolecules, 2021 Nostocyclopeptides (Ncps) are a small class of bioactive nonribosomal peptides produced solely by cyanobacteria of the genus Nostoc. In the current work, six Ncps were isolated from Nostoc edaphicum strain CCNP1411. The bioactivity of these compounds was tested in vitro against 20S proteasome, a proteolytic complex that plays an important role in ...
Fidor A +6 more
europepmc +7 more sources
The ubiquitin-like modifier FAT10 is degraded by the 20S proteasome in vitro but not in cellulo [PDF]
Life Science Alliance, 2023 The ubiquitin-like modifier FAT10 is degraded by the 20S proteasome in vitro, whereas FAT10 degradation depends on the 26S proteasome in cellulo, shown by impairing 26S function via Rpt2 knockdown.
Franziska Oliveri +4 more
doaj +2 more sources
Structural Insights into Salinosporamide a Mediated Inhibition of the Human 20S Proteasome [PDF]
MoleculesThe 20S proteasome, a critical component of the ubiquitin–proteasome system, plays a central role in regulating protein degradation in eukaryotic cells.
Hagen Sülzen +4 more
doaj +2 more sources
Glucose restriction in Saccharomyces cerevisiae modulates the phosphorylation pattern of the 20S proteasome and increases its activity [PDF]
Scientific Reports, 2023 Caloric restriction is known to extend the lifespan and/or improve diverse physiological parameters in a vast array of organisms. In the yeast Saccharomyces cerevisiae, caloric restriction is performed by reducing the glucose concentration in the culture
Renata Naporano Bicev +8 more
doaj +2 more sources
Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family [PDF]
Nature Communications, 2023 Controlled degradation of proteins is necessary for ensuring their abundance and sustaining a healthy and accurately functioning proteome. One of the degradation routes involves the uncapped 20S proteasome, which cleaves proteins with a partially ...
Fanindra Kumar Deshmukh +10 more
doaj +2 more sources
Regulating the 20S Proteasome Ubiquitin-Independent Degradation Pathway
Biomolecules, 2014 For many years, the ubiquitin-26S proteasome degradation pathway was considered the primary route for proteasomal degradation. However, it is now becoming clear that proteins can also be targeted for degradation by the core 20S proteasome itself ...
Gili Ben-Nissan, Michal Sharon
doaj +3 more sources
Establishing 20S Proteasome Genetic, Translational and Post-Translational Status from Precious Biological and Patient Samples with Top-Down MS [PDF]
Cells, 2023 The mammalian 20S catalytic core of the proteasome is made of 14 different subunits (α1-7 and β1-7) but exists as different subtypes depending on the cell type.
Angelique Sanchez Dafun +17 more
doaj +2 more sources
The ATP/Mg2+ Balance Affects the Degradation of Short Fluorogenic Substrates by the 20S Proteasome [PDF]
Methods and Protocols, 2022 Proteasomes hydrolyze most cellular proteins. The standard reaction to determine proteasome activity in cellular lysate or elsewhere contains AMC-conjugated peptide substrate, ATP, Mg2+, and DTT.
Alexey Morozov +3 more
doaj +2 more sources