The Contribution of the 20S Proteasome to Proteostasis [PDF]
Biomolecules, 2019 The last decade has seen accumulating evidence of various proteins being degraded by the core 20S proteasome, without its regulatory particle(s). Here, we will describe recent advances in our knowledge of the functional aspects of the 20S proteasome ...
Fanindra Kumar Deshmukh +2 more
exaly +6 more sources
Systematic Identification of 20S Proteasome Substrates [PDF]
Molecular Systems BiologyFor years, proteasomal degradation was predominantly attributed to the ubiquitin-26S proteasome pathway. However, it is now evident that the core 20S proteasome can independently target proteins for degradation.
Paola Picotti +2 more
exaly +6 more sources
Regulating the 20S Proteasome Ubiquitin-Independent Degradation Pathway
Biomolecules, 2014 For many years, the ubiquitin-26S proteasome degradation pathway was considered the primary route for proteasomal degradation. However, it is now becoming clear that proteins can also be targeted for degradation by the core 20S proteasome itself ...
Gili Ben Nissan, Michal Sharon
exaly +4 more sources
Structural Insights into Salinosporamide a Mediated Inhibition of the Human 20S Proteasome [PDF]
MoleculesThe 20S proteasome, a critical component of the ubiquitin–proteasome system, plays a central role in regulating protein degradation in eukaryotic cells.
Hagen Sülzen +4 more
doaj +3 more sources
The ubiquitin-like modifier FAT10 is degraded by the 20S proteasome in vitro but not in cellulo [PDF]
Life Science Alliance, 2023 The ubiquitin-like modifier FAT10 is degraded by the 20S proteasome in vitro, whereas FAT10 degradation depends on the 26S proteasome in cellulo, shown by impairing 26S function via Rpt2 knockdown.
Franziska Oliveri +4 more
doaj +2 more sources
Glucose restriction in Saccharomyces cerevisiae modulates the phosphorylation pattern of the 20S proteasome and increases its activity [PDF]
Scientific Reports, 2023 Caloric restriction is known to extend the lifespan and/or improve diverse physiological parameters in a vast array of organisms. In the yeast Saccharomyces cerevisiae, caloric restriction is performed by reducing the glucose concentration in the culture
Renata Naporano Bicev +8 more
doaj +2 more sources
Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family [PDF]
Nature Communications, 2023 Controlled degradation of proteins is necessary for ensuring their abundance and sustaining a healthy and accurately functioning proteome. One of the degradation routes involves the uncapped 20S proteasome, which cleaves proteins with a partially ...
Fanindra Kumar Deshmukh +10 more
doaj +2 more sources
The preliminary study suggests an association between NF-ĸB pathway activation and increased plasma 20S proteasome activity in intracranial aneurysm patients [PDF]
Scientific ReportsThe significant role of increased activation of 20S proteasomes in the development of abdominal aortic aneurysms has been well-established in a mouse model. The available literature lacks similar studies concerning brain aneurysms.
Joanna Kamińska +12 more
doaj +2 more sources
Limiting 20S proteasome assembly leads to unbalanced nucleo-cytoplasmic distribution of 26S/30S proteasomes and chronic proteotoxicity [PDF]
iScienceSummary: In addition to the degradation of cell-cycle proteins, short-lived, damaged, or unfolded proteins are constantly cleared from cells by the proteasome.
Gabriel Ruiz-Romero +4 more
doaj +2 more sources
In vitro Reconstitution Assays of Arabidopsis 20S Proteasome
Bio-Protocol, 2021 The majority of cellular proteins are degraded by the 26S proteasome in eukaryotes. However, intrinsically disordered proteins (IDPs), which contain large portions of unstructured regions and are inherently unstable, are degraded via the ubiquitin ...
Yanjun Li +4 more
doaj +1 more source