Results 41 to 50 of about 80,587 (292)

The proteasome and the degradation of oxidized proteins: Part I—structure of proteasomes

Redox Biology, 2013
The main machinery responsible for cellular protein maintenance is the ubiquitin-proteasomal system, with its core particle the 20S proteasome. The main task of the system is a fast and efficient degradation of proteins not needed anymore in cellular ...
Tobias Jung, Tilman Grune
doaj   +1 more source

The ATP/Mg²⁺ Balance Affects the Degradation of Short Fluorogenic Substrates by the 20S Proteasome

Methods and Protocols, 2022
Proteasomes hydrolyze most cellular proteins. The standard reaction to determine proteasome activity in cellular lysate or elsewhere contains AMC-conjugated peptide substrate, ATP, Mg2+, and DTT.
Alexey Morozov, Tatyana Astakhova, Pavel Erokhov, Vadim Karpov   +3 more
doaj   +1 more source

The proteasome inhibitor PI31 competes with PA28 for binding to 20S proteasomes [PDF]

FEBS Letters, 1999
PI31 is a previously described inhibitor of 20S proteasomes. Using recombinant PI31 we have analyzed its effect on proteasomal hydrolyzing activity of short fluorogenic substrates and of a synthetic 40‐mer polypeptide. In addition, we investigated its influence on the activation of 20S proteasome by the proteasome activator PA28.
Zaiss, D M, Standera, Sybille, Holzhütter, H, Kloetzel, P.M., Sijts, A J   +4 more
openaire   +3 more sources

Method of Monitoring 26S Proteasome in Cells Revealed the Crucial Role of PSMA3 C-Terminus in 26S Integrity

Biomolecules, 2023
Proteasomes critically regulate proteostasis via protein degradation. Proteasomes are multi-subunit complexes composed of the 20S proteolytic core particle (20S CP) that, in association with one or two 19S regulatory particles (19S RPs), generates the ...
Shirel Steinberger, Julia Adler, Yosef Shaul   +2 more
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The β-Grasp Domain of Proteasomal ATPase Mpa Makes Critical Contacts with the Mycobacterium tuberculosis 20S Core Particle to Facilitate Degradation

mSphere, 2022
Mycobacterium tuberculosis possesses a Pup-proteasome system analogous to the eukaryotic ubiquitin-proteasome pathway. We have previously shown that the hexameric mycobacterial proteasome ATPase (Mpa) recruits pupylated protein substrates via ...
Xiansha Xiao, Xiang Feng, Jin Hee Yoo, Amanda Kovach, K. Heran Darwin, Huilin Li   +5 more
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Metabolism of Alpha-Synuclein by the 20S Proteasome [PDF]

, 2009
Parkinson's disease is one of several common neurodegenerative disorders that are related by the intracellular aggregation of the neuronal protein alpha-synuclein (alphaSyn) that normally associates with synaptic vesicles. Within the aggregates,
Lewis, Karen Adell
core   +3 more sources

Minimal mechanistic component of HbYX-dependent proteasome activation that reverses impairment by neurodegenerative-associated oligomers

Communications Biology, 2023
The implication of reduced proteasomal function in neurodegenerative diseases combined with studies showing the protective effects of increasing proteasome activity in animal models highlight the need to understand the capacity for proteasome activation ...
Janelle J. Y. Chuah, Tiffany A. Thibaudeau, David M. Smith   +2 more
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Peptidomimetics Based on C-Terminus of Blm10 Stimulate Human 20S Proteasome Activity and Promote Degradation of Proteins

Biomolecules, 2022
Degradation of misfolded, redundant and oxidatively damaged proteins constitutes one of the cellular processes which are influenced by the 20S proteasome.
Katarzyna Cekała, Karolina Trepczyk, Daria Sowik, Przemysław Karpowicz, Artur Giełdoń, Julia Witkowska, Małgorzata Giżyńska, Elżbieta Jankowska, Ewa Wieczerzak   +8 more
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Subunit arrangement in the human 20S proteasome [PDF]

Proceedings of the National Academy of Sciences, 1997
In human 20S proteasomes two copies of each of seven different α-type and seven different β-type subunits are assembled to form a stack of four seven-membered rings, giving the general structure α 1–7 , β 1–7 , β 1–7
Kopp, Friedrich, Hendil, Klaus Aksel Bjørner, Dahlmann, Burkhardt, Kristensen, Poul, Sobek, Axel, Uerkvitz, Wolfgang   +5 more
openaire   +2 more sources

Silencing of the 20S proteasomal subunit-α6 triggers full oogenesis arrest and increased mRNA levels of the selective autophagy adaptor protein p62/SQSTM1 in the ovary of the vector Rhodnius prolixus.

PLoS Neglected Tropical Diseases, 2023
The high reproductive rates of insects contribute significantly to their ability to act as vectors of a variety of vector-borne diseases. Therefore, it is strategically critical to find molecular targets with biotechnological potential through the ...
Allana Faria-Reis, Samara Santos-Araújo, Jéssica Pereira, Thamara Rios, David Majerowicz, Katia C Gondim, Isabela Ramos   +6 more
doaj   +1 more source