Results 11 to 20 of about 607,065 (259)
Deterministic culturing of single cells in 3D [PDF]
Scientific Reports, 2020 AbstractModels using 3D cell culture techniques are increasingly accepted as the most biofidelic in vitro representations of tissues for research. These models are generated using biomatrices and bulk populations of cells derived from tissues or cell lines. We present an alternate method to culture individually selected cells in relative isolation from
Rohil Jain +4 more
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European Journal of Cell Biology, 2022 Mesenchymal stem cells (MSCs) show a decline in pluripotency and differentiation with increased cell culture passages in 2D cultures. The 2D monolayer culture fails to correctly imitate the architecture and microenvironments of in-vivo cell models ...
Gitika Thakur +10 more
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Pharmaceuticals, 2022 Despite tremendous advancements in technologies and resources, drug discovery still remains a tedious and expensive process. Though most cells are cultured using 2D monolayer cultures, due to lack of specificity, biochemical incompatibility, and cell-to ...
Shaimaa M. Badr-Eldin +4 more
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Biosensors, 2022 Cell culture plays an essential role in tissue engineering and high-throughput drug screening. Compared with two-dimensional (2D) in vitro culture, three-dimensional (3D) in vitro culture can mimic cells in vivo more accurately, including complex ...
Chunyang Wei +5 more
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Bioactive Materials, 2023 Three-dimensional (3D) stem cell culture systems have attracted considerable attention as a way to better mimic the complex interactions between individual cells and the extracellular matrix (ECM) that occur in vivo.
Woochan Kim +4 more
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3D Cell Culture in Alginate Hydrogels [PDF]
Microarrays, 2015 This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of
Andersen, Therese +2 more
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Microenvironment-Cell Nucleus Relationship in the Context of Oxidative Stress
Frontiers in Cell and Developmental Biology, 2018 The microenvironment is a source of reactive oxygen species (ROS) that influence cell phenotype and tissue homeostasis. The impact of ROS on redox pathways as well as directly on epigenetic mechanisms and the DNA illustrate communication with the cell ...
Shirisha Chittiboyina +5 more
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Development of Tissue Engineering Scaffolds for Cancer Cell Cultures [PDF]
Journal of Mazandaran University of Medical Sciences, 2023 The cell culture technique is one of the tools used in cellular and molecular biology such as cancer cell culture, regenerative medicine, and drug discovery.
Seyede Maryam Valizadeh Otaghsara +5 more
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Cells, 2022 The two-dimensional (2D) monolayer culture as a conventional method has been widely applied in molecular biology fields, but it has limited capability to recapitulate real cell environments, being prone to misinterpretation with poor prediction of in ...
Ji Min Oh +5 more
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Skeletal Muscle, 2019 Background Trauma-induced heterotopic ossification (HO) is a complication that develops under three conditions: the presence of an osteogenic progenitor cell, an inducing factor, and a permissive environment. We previously showed that a mouse multipotent
Geneviève Drouin +5 more
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