Results 11 to 20 of about 609,890 (261)

Three-Dimensional In Vitro Cell Culture Models for Efficient Drug Discovery: Progress So Far and Future Prospects

Pharmaceuticals, 2022
Despite tremendous advancements in technologies and resources, drug discovery still remains a tedious and expensive process. Though most cells are cultured using 2D monolayer cultures, due to lack of specificity, biochemical incompatibility, and cell-to ...
Shaimaa M. Badr-Eldin, Hibah M. Aldawsari, Sabna Kotta, Pran Kishore Deb, Katharigatta N. Venugopala   +4 more
doaj   +1 more source

Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration

Bioactive Materials, 2023
Three-dimensional (3D) stem cell culture systems have attracted considerable attention as a way to better mimic the complex interactions between individual cells and the extracellular matrix (ECM) that occur in vivo.
Woochan Kim, Yonghyun Gwon, Sunho Park, Hyoseong Kim, Jangho Kim   +4 more
doaj   +1 more source

Easy-to-Operate Co-Flow Step Emulsification Device for High-Throughput Three-Dimensional Cell Culture

Biosensors, 2022
Cell culture plays an essential role in tissue engineering and high-throughput drug screening. Compared with two-dimensional (2D) in vitro culture, three-dimensional (3D) in vitro culture can mimic cells in vivo more accurately, including complex ...
Chunyang Wei, Chengzhuang Yu, Shanshan Li, Tiejun Li, Jiyu Meng, Junwei Li   +5 more
doaj   +1 more source

3D Cell Culture in Alginate Hydrogels [PDF]

Microarrays, 2015
This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of
Andersen, Therese, Auk-Emblem, Pia, Dornish, Michael   +2 more
openaire   +2 more sources

Development of Tissue Engineering Scaffolds for Cancer Cell Cultures [PDF]

Journal of Mazandaran University of Medical Sciences, 2023
The cell culture technique is one of the tools used in cellular and molecular biology such as cancer cell culture, regenerative medicine, and drug discovery.
Seyede Maryam Valizadeh Otaghsara, Hassan Dariushnejad, Amir Mellati, Elham Hasanzadeh, Mozhgan Abasi, Pedram Ebrahimnejad   +5 more
doaj  

Microenvironment-Cell Nucleus Relationship in the Context of Oxidative Stress

Frontiers in Cell and Developmental Biology, 2018
The microenvironment is a source of reactive oxygen species (ROS) that influence cell phenotype and tissue homeostasis. The impact of ROS on redox pathways as well as directly on epigenetic mechanisms and the DNA illustrate communication with the cell ...
Shirisha Chittiboyina, Shirisha Chittiboyina, Yunfeng Bai, Sophie A. Lelièvre, Sophie A. Lelièvre, Sophie A. Lelièvre   +5 more
doaj   +1 more source

Different Expression of Thyroid-Specific Proteins in Thyroid Cancer Cells between 2-Dimensional (2D) and 3-Dimensional (3D) Culture Environment

Cells, 2022
The two-dimensional (2D) monolayer culture as a conventional method has been widely applied in molecular biology fields, but it has limited capability to recapitulate real cell environments, being prone to misinterpretation with poor prediction of in ...
Ji Min Oh, Prakash Gangadaran, Ramya Lakshmi Rajendran, Chae Moon Hong, Jaetae Lee, Byeong-Cheol Ahn   +5 more
doaj   +1 more source

Muscle injury-induced hypoxia alters the proliferation and differentiation potentials of muscle resident stromal cells

Skeletal Muscle, 2019
Background Trauma-induced heterotopic ossification (HO) is a complication that develops under three conditions: the presence of an osteogenic progenitor cell, an inducing factor, and a permissive environment. We previously showed that a mouse multipotent
Geneviève Drouin, Vanessa Couture, Marc-Antoine Lauzon, Frédéric Balg, Nathalie Faucheux, Guillaume Grenier   +5 more
doaj   +1 more source

Mesenchymal stem cells lose the senescent phenotype under 3D cultivation

Stem Cell Research & Therapy, 2023
Background Three-dimensional (3D) cell culture is widely used in various fields of cell biology. In comparison to conventional two-dimensional (2D) cell culture, 3D cell culture facilitates a more accurate replication of the in vivo microenvironment ...
O. Krasnova, A. Kovaleva, A. Saveleva, K. Kulakova, O. Bystrova, M. Martynova, A. Domnina, J. Sopova, I. Neganova   +8 more
doaj   +1 more source

3-Dimensional mesothelioma spheroids provide closer to natural pathophysiological tumor microenvironment for drug response studies

Frontiers in Oncology, 2022
Traditional studies using cancer cell lines are often performed on a two-dimensional (2D) cell culture model with a low success rate of translating to Phase I or Phase II clinical studies. In comparison, with the advent of developments three-dimensional (
Huaikai Shi, Emma M. Rath, Ruby C. Y. Lin, Ruby C. Y. Lin, Ruby C. Y. Lin, Kadir Harun Sarun, Candice Julie Clarke, Brian C. McCaughan, Brian C. McCaughan, Helen Ke, Helen Ke, Anthony Linton, Anthony Linton, Anthony Linton, Kenneth Lee, Kenneth Lee, Sonja Klebe, Sonja Klebe, Joanneke Maitz, Joanneke Maitz, Joanneke Maitz, Kedong Song, Yiwei Wang, Yiwei Wang, Steven Kao, Steven Kao, Yuen Yee Cheng   +26 more
doaj   +1 more source