Results 11 to 20 of about 10,804,267 (253)
European Journal of Cell Biology, 2022 Mesenchymal stem cells (MSCs) show a decline in pluripotency and differentiation with increased cell culture passages in 2D cultures. The 2D monolayer culture fails to correctly imitate the architecture and microenvironments of in-vivo cell models ...
Gitika Thakur +10 more
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Pharmaceuticals, 2022 Despite tremendous advancements in technologies and resources, drug discovery still remains a tedious and expensive process. Though most cells are cultured using 2D monolayer cultures, due to lack of specificity, biochemical incompatibility, and cell-to ...
Shaimaa M. Badr-Eldin +4 more
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Vascularization in 3D Cell Culture
, 2021 The two-dimensional (2D) monoculture does not recapitulate the three-dimensional (3D) environment of in vivo vasculature. Endothelial cell (ECs) spheroids have been introduced as a 3D in vitro model exhibiting angiogenic responses and sprouting behavior ...
Bagli, E. +9 more
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Biosensors, 2022 Cell culture plays an essential role in tissue engineering and high-throughput drug screening. Compared with two-dimensional (2D) in vitro culture, three-dimensional (3D) in vitro culture can mimic cells in vivo more accurately, including complex ...
Chunyang Wei +5 more
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Bioactive Materials, 2023 Three-dimensional (3D) stem cell culture systems have attracted considerable attention as a way to better mimic the complex interactions between individual cells and the extracellular matrix (ECM) that occur in vivo.
Woochan Kim +4 more
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In Vitro three-dimensional (3D) cell culture tools for spheroid and organoid models
SLAS Discovery, 2023 Three-dimensional (3D) cell culture technology has been steadily studied since the 1990′s due to its superior biocompatibility compared to the conventional two-dimensional (2D) cell culture technology, and has recently developed into an organoid culture ...
Sang-Yun Lee +3 more
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Development of Tissue Engineering Scaffolds for Cancer Cell Cultures [PDF]
Journal of Mazandaran University of Medical Sciences, 2023 The cell culture technique is one of the tools used in cellular and molecular biology such as cancer cell culture, regenerative medicine, and drug discovery.
Seyede Maryam Valizadeh Otaghsara +5 more
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Microenvironment-Cell Nucleus Relationship in the Context of Oxidative Stress
Frontiers in Cell and Developmental Biology, 2018 The microenvironment is a source of reactive oxygen species (ROS) that influence cell phenotype and tissue homeostasis. The impact of ROS on redox pathways as well as directly on epigenetic mechanisms and the DNA illustrate communication with the cell ...
Shirisha Chittiboyina +5 more
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Cells, 2022 The two-dimensional (2D) monolayer culture as a conventional method has been widely applied in molecular biology fields, but it has limited capability to recapitulate real cell environments, being prone to misinterpretation with poor prediction of in ...
Ji Min Oh +5 more
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Is It Time to Start Transitioning From 2D to 3D Cell Culture?
Frontiers in Molecular Biosciences, 2020 Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D ...
Caleb Jensen +5 more
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