Results 31 to 40 of about 609,890 (261)

Multiple cystic sphere formation from PK-8 cells in three-dimensional culture

Biochemistry and Biophysics Reports, 2022
Three-dimensional (3D) culture of cancer cells mimics the in vivo environment. Recently, we reported that pancreatic ductal adenocarcinoma (PDAC) cell lines with epithelial and mesenchymal features formed differently shaped spheres in 3D culture. However,
Yuuki Shichi, Fujiya Gomi, Yoshibumi Ueda, Keisuke Nonaka, Fumio Hasegawa, Yasuko Hasegawa, Nae Hinata, Hisashi Yoshimura, Masami Yamamoto, Kimimasa Takahashi, Tomio Arai, Toshiyuki Ishiwata   +11 more
doaj   +1 more source

Magnetic Stiffening in 3D Cell Culture Matrices [PDF]

Nano Letters, 2021
The mechanical environment of a cell is not constant. This dynamic behavior is exceedingly difficult to capture in (synthetic) in vitro matrices. This paper describes a novel, highly adaptive hybrid hydrogel composed of magnetically sensitive magnetite nanorods and a stress-responsive synthetic matrix. Nanorod rearrangement after application of (small)
Wen Chen, Ying Zhang, Jyoti Kumari, Hans Engelkamp, Paul H. J. Kouwer   +4 more
openaire   +4 more sources

A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue

Frontiers in Bioengineering and Biotechnology, 2021
Three-dimensional (3D) co-culture models have closer physiological cell composition and behavior than traditional 2D culture. They exhibit pharmacological effects like in vivo responses, and therefore serve as a high-throughput drug screening model to ...
Jietao Lin, Jietao Lin, Jietao Lin, Antonia RuJia Sun, Antonia RuJia Sun, Antonia RuJia Sun, Jian Li, Jian Li, Jian Li, Tianying Yuan, Wenxiang Cheng, Wenxiang Cheng, Wenxiang Cheng, Liqing Ke, Liqing Ke, Liqing Ke, Jianhai Chen, Jianhai Chen, Jianhai Chen, Wei Sun, Shengli Mi, Peng Zhang, Peng Zhang, Peng Zhang   +23 more
doaj   +1 more source

Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

Frontiers in Bioengineering and Biotechnology
Recent advancements in 3D bioprinting and biofabrication offer promising avenues for tissue engineering (TE) and regenerative medicine (RM), particularly in addressing musculoskeletal conditions such as articular cartilage lesions.
Iván López-González, Iván López-González, Noelia Campillo, José Manuel Baena, José Manuel Baena, José Manuel Baena   +5 more
doaj   +1 more source

Organotypic 3D Cell-Architecture Impacts the Expression Pattern of miRNAs–mRNAs Network in Breast Cancer SKBR3 Cells

Non-Coding RNA, 2023
Background. Currently, most of the research on breast cancer has been carried out in conventional two-dimensional (2D) cell cultures due to its practical benefits, however, the three-dimensional (3D) cell culture is becoming the model of choice in cancer
María de los Ángeles Gastélum-López, Maribel Aguilar-Medina, Cristina García Mata, Jorge López-Gutiérrez, Geovanni Romero-Quintana, Mercedes Bermúdez, Mariana Avendaño-Felix, César López-Camarillo, Carlos Pérez-Plascencia, Adriana S Beltrán, Rosalío Ramos-Payán   +10 more
doaj   +1 more source

3D CULTURE OF HaCaT KERATINOCYTE CELL LINE AS AN in vitro TOXICITY MODEL

Trakya University Journal of Natural Sciences, 2022
Ex vivo dermal toxicology analyses are crucial for replacement of in vivo test methods and have been of interest in recent years for testing cosmetics, drugs, and chemicals.
Fikrettin Şahin, Engin Sümer, Burçin Asutay, Derya Sağraç, Hatice Burcu Şişli, Binnur Kıratlı, Taha Bartu Hayal, Derya Burukçu, Selinay Şenkal, Ayşegül Doğan   +9 more
doaj   +1 more source

3D culture for cardiac cells

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2016
This review discusses historical milestones, recent developments and challenges in the area of 3D culture models with cardiovascular cell types. Expectations in this area have been raised in recent years, but more relevant in vitro research, more accurate drug testing results, reliable disease models and insights leading to bioartificial organs are ...
openaire   +3 more sources

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source