Results 31 to 40 of about 10,293,161 (301)

Enhanced differentiation of human pluripotent stem cells into pancreatic endocrine cells in 3D culture by inhibition of focal adhesion kinase

Stem Cell Research & Therapy, 2020
Background Generation of insulin-producing cells from human pluripotent stem cells (hPSCs) in vitro would be useful for drug discovery and cell therapy in diabetes.
Xiaofang Liu, Jinhua Qin, Mingyang Chang, Shuyong Wang, Yali Li, Xuetao Pei, Yunfang Wang   +6 more
doaj   +1 more source

3D cell culture model: From ground experiment to microgravity study

Frontiers in Bioengineering and Biotechnology, 2023
Microgravity has been shown to induce many changes in cell growth and differentiation due to offloading the gravitational strain normally exerted on cells.
Chiyuan Ma, Chiyuan Ma, Xianglong Duan, Xianglong Duan, Xiaohua Lei   +4 more
doaj   +1 more source

Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells

Data in Brief, 2015
Three-dimensional (3D) cell culture is a powerful tool to study cell growth under 3D condition. To perform a simple test for anti-cancer drugs in 3D culture, visualization of non-proliferated cells is required.
Junji Itou, Sunao Tanaka, Wenzhao Li, Yoshiaki Matsumoto, Fumiaki Sato, Masakazu Toi   +5 more
doaj   +1 more source

A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue

Frontiers in Bioengineering and Biotechnology, 2021
Three-dimensional (3D) co-culture models have closer physiological cell composition and behavior than traditional 2D culture. They exhibit pharmacological effects like in vivo responses, and therefore serve as a high-throughput drug screening model to ...
Jietao Lin, Jietao Lin, Jietao Lin, Antonia RuJia Sun, Antonia RuJia Sun, Antonia RuJia Sun, Jian Li, Jian Li, Jian Li, Tianying Yuan, Wenxiang Cheng, Wenxiang Cheng, Wenxiang Cheng, Liqing Ke, Liqing Ke, Liqing Ke, Jianhai Chen, Jianhai Chen, Jianhai Chen, Wei Sun, Shengli Mi, Peng Zhang, Peng Zhang, Peng Zhang   +23 more
doaj   +1 more source

Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture

, 2022
In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to ...
Marvin Corea, Magdalena Metzger, Schädl, Barbara, Grillari, Johannes, Dungel, Peter, Peter Dungel, Johannes Grillari, Bauer, Magdalena, Barbara Schädl, Metzger, Magdalena, Corea, Marvin, Magdalena Bauer   +11 more
core   +1 more source

Magnetic Stiffening in 3D Cell Culture Matrices [PDF]

Nano Letters, 2021
The mechanical environment of a cell is not constant. This dynamic behavior is exceedingly difficult to capture in (synthetic) in vitro matrices. This paper describes a novel, highly adaptive hybrid hydrogel composed of magnetically sensitive magnetite nanorods and a stress-responsive synthetic matrix. Nanorod rearrangement after application of (small)
Wen Chen, Ying Zhang, Jyoti Kumari, Hans Engelkamp, Paul H. J. Kouwer   +4 more
openaire   +4 more sources

Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs

Frontiers in Bioengineering and Biotechnology
Recent advancements in 3D bioprinting and biofabrication offer promising avenues for tissue engineering (TE) and regenerative medicine (RM), particularly in addressing musculoskeletal conditions such as articular cartilage lesions.
Iván López-González, Iván López-González, Noelia Campillo, José Manuel Baena, José Manuel Baena, José Manuel Baena   +5 more
doaj   +1 more source

3D time series analysis of cell shape using Laplacian approaches [PDF]

, 2013
Background: Fundamental cellular processes such as cell movement, division or food uptake critically depend on cells being able to change shape. Fast acquisition of three-dimensional image time series has now become possible, but we lack efficient tools
Stephens, Len R., Len R Stephens, Bretschneider, Till, Phillip T Hawkins, Cheng-Jin Du, Hawkins, Phillip T., Du, Cheng-Jin, Till Bretschneider   +7 more
core   +1 more source

Organotypic 3D Cell-Architecture Impacts the Expression Pattern of miRNAs–mRNAs Network in Breast Cancer SKBR3 Cells

Non-Coding RNA, 2023
Background. Currently, most of the research on breast cancer has been carried out in conventional two-dimensional (2D) cell cultures due to its practical benefits, however, the three-dimensional (3D) cell culture is becoming the model of choice in cancer
María de los Ángeles Gastélum-López, Maribel Aguilar-Medina, Cristina García Mata, Jorge López-Gutiérrez, Geovanni Romero-Quintana, Mercedes Bermúdez, Mariana Avendaño-Felix, César López-Camarillo, Carlos Pérez-Plascencia, Adriana S Beltrán, Rosalío Ramos-Payán   +10 more
doaj   +1 more source

Multiple cystic sphere formation from PK-8 cells in three-dimensional culture

Biochemistry and Biophysics Reports, 2022
Three-dimensional (3D) culture of cancer cells mimics the in vivo environment. Recently, we reported that pancreatic ductal adenocarcinoma (PDAC) cell lines with epithelial and mesenchymal features formed differently shaped spheres in 3D culture. However,
Yuuki Shichi, Fujiya Gomi, Yoshibumi Ueda, Keisuke Nonaka, Fumio Hasegawa, Yasuko Hasegawa, Nae Hinata, Hisashi Yoshimura, Masami Yamamoto, Kimimasa Takahashi, Tomio Arai, Toshiyuki Ishiwata   +11 more
doaj   +1 more source