Results 51 to 60 of about 607,065 (259)

A new inject-embed 3D culture method enables the spheroid and aggregate formation from single or dual liver cancer cell types

Cancer Cell International
Background Three-dimensional (3D) cell culture techniques have emerged as a bridge between traditional two-dimensional (2D) cell culture and the complex 3D architecture of living organisms.
Hanyuan Zhang, Shuting Xue, Jinchang Pan, Jianjuan Zhang, Danduo Wei, Peng Xia, Guangming Li, Stephanie Roessler, Chaohui Yu, Junfang Ji   +9 more
doaj   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Evaluation of MCF10A as a Reliable Model for Normal Human Mammary Epithelial Cells. [PDF]

PLoS ONE, 2015
Breast cancer is the most common cancer in women and a leading cause of cancer-related deaths for women worldwide. Various cell models have been developed to study breast cancer tumorigenesis, metastasis, and drug sensitivity.
Ying Qu, Bingchen Han, Yi Yu, Weiwu Yao, Shikha Bose, Beth Y Karlan, Armando E Giuliano, Xiaojiang Cui   +7 more
doaj   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Breast Cancer Stem Cell Culture and Enrichment Using Poly(ε-Caprolactone) Scaffolds

Molecules, 2016
The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs’ phenotype and functions is needed to investigate new therapeutic
Sònia Palomeras, Marc Rabionet, Inés Ferrer, Ariadna Sarrats, Maria Luisa Garcia-Romeu, Teresa Puig, Joaquim Ciurana   +6 more
doaj   +1 more source

Glutathione-triggered formation of a Fmoc-protected short peptide-based supramolecular hydrogel. [PDF]

PLoS ONE, 2014
A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine ...
Yang Shi, Jingyu Wang, Huaimin Wang, Yanhui Hu, Xuemei Chen, Zhimou Yang   +5 more
doaj   +1 more source

Three-Dimensional Reconstructed Bone Marrow Matrix Culture Improves the Viability of Primary Myeloma Cells In-Vitro via a STAT3-Dependent Mechanism

Current Issues in Molecular Biology, 2021
Primary myeloma (PM) cells are short-lived in conventional culture, which limited their usefulness as a study model. Here, we evaluated if three-dimensional (3D) culture can significantly prolong the longevity of PM cells in-vitro.
Yung-Hsing Huang, Meaad Almowaled, Jing Li, Christopher Venner, Irwindeep Sandhu, Anthea Peters, Afsaneh Lavasanifar, Raymond Lai   +7 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. [PDF]

PLoS ONE, 2017
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells.
Hua Mei, Sheyla González, Martin N Nakatsu, Elfren R Baclagon, Felix V Chen, Sophie X Deng   +5 more
doaj   +1 more source