Results 61 to 70 of about 10,293,161 (301)

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Evaluation of MCF10A as a Reliable Model for Normal Human Mammary Epithelial Cells. [PDF]

PLoS ONE, 2015
Breast cancer is the most common cancer in women and a leading cause of cancer-related deaths for women worldwide. Various cell models have been developed to study breast cancer tumorigenesis, metastasis, and drug sensitivity.
Ying Qu, Bingchen Han, Yi Yu, Weiwu Yao, Shikha Bose, Beth Y Karlan, Armando E Giuliano, Xiaojiang Cui   +7 more
doaj   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Glutathione-triggered formation of a Fmoc-protected short peptide-based supramolecular hydrogel. [PDF]

PLoS ONE, 2014
A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine ...
Yang Shi, Jingyu Wang, Huaimin Wang, Yanhui Hu, Xuemei Chen, Zhimou Yang   +5 more
doaj   +1 more source

Three-Dimensional Reconstructed Bone Marrow Matrix Culture Improves the Viability of Primary Myeloma Cells In-Vitro via a STAT3-Dependent Mechanism

Current Issues in Molecular Biology, 2021
Primary myeloma (PM) cells are short-lived in conventional culture, which limited their usefulness as a study model. Here, we evaluated if three-dimensional (3D) culture can significantly prolong the longevity of PM cells in-vitro.
Yung-Hsing Huang, Meaad Almowaled, Jing Li, Christopher Venner, Irwindeep Sandhu, Anthea Peters, Afsaneh Lavasanifar, Raymond Lai   +7 more
doaj   +1 more source

3D bioprinting of hydrogels for retina cell culturing [PDF]

Bioprinting, 2018
Recapitulating native retina environment is crucial in isolation and culturing of retina photoreceptors (PRs). To date, maturation of PRs remains incomprehensible in vitro. Here we present a strategy of integrating the physical and chemical signals through 3D-bioprinting of hyaluronic acid (HA) hydrogels and co-differentiation of retinal progenitor ...
Pengrui Wang, Xin Li, Wei Zhu, Zheng Zhong, Amy Moran, Wenqiu Wang, Kang Zhang, Shaochen Chen   +7 more
openaire   +2 more sources

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Differentiation of stem cell-derived pancreatic progenitors into insulin-secreting islet clusters in a multiwell-based static 3D culture system

, 2023
Orbital shaker-based suspension culture systems have been in widespread use for differentiating human pluripotent stem cell (hPSC)-derived pancreatic progenitors toward islet-like clusters during endocrine induction stages.
Zhan, Lisa, Timothy J. Kieffer, Jia Zhao, Robert K. Baker, Baker, Robert K, Elisa Tran, Zhao, Jia, Shenghui Liang, Tran, Elisa, Lisa Zhan, Liang, Shenghui, Kieffer, Timothy J   +11 more
core   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. [PDF]

PLoS ONE, 2017
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells.
Hua Mei, Sheyla González, Martin N Nakatsu, Elfren R Baclagon, Felix V Chen, Sophie X Deng   +5 more
doaj   +1 more source