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Esophageal stem cells and 3D‐cell culture models
Annals of the New York Academy of Sciences, 2011The following on esophageal stem cells and 3D‐cell culture models contains commentaries on metaplasia through transdifferentiation and through stem cells; transcription factors that may determine an intestinal‐like phenotype; the in vitro, organotypic cell culture models; and the role of stem cells in Barrett's esophagus and its dysplastic progression.
Rhonda F, Souza +2 more
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Electromolding for 3D cell culture
10th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, 2015Constructing microstructures with crosslinkable biomaterials, gelatin methacrylate (GelMA) and poly(ethylene glycol) diacrylate (PEGDA), containing fibroblasts (NIH-3T3) are demonstrated using electromolding that combines electrowetting and dielectrophoresis on an electromicrofluidic platform without physical molds.
Yi-Han Lai, Shih-Kang Fan
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Vascularization in 3D Cell Culture
2021The two-dimensional (2D) monoculture does not recapitulate the three-dimensional (3D) environment of in vivo vasculature. Endothelial cell (ECs) spheroids have been introduced as a 3D in vitro model exhibiting angiogenic responses and sprouting behavior in vivo.
M. Markou +4 more
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2017
In the 1960s, scientists searching for a cell-based system that would reflect the physiology and heterogeneity of solid tumors in a well-controlled experimental setting developed multicell spheroid cultures. Early studies focused on the biology, physiology, and drug and radiation response of spheroids.
David M. Evans, Beverly A. Teicher
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In the 1960s, scientists searching for a cell-based system that would reflect the physiology and heterogeneity of solid tumors in a well-controlled experimental setting developed multicell spheroid cultures. Early studies focused on the biology, physiology, and drug and radiation response of spheroids.
David M. Evans, Beverly A. Teicher
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Viscoelastic hydrogels for 3D cell culture
Biomaterials Science, 2017This mini-review discusses newly developed approaches to tuning hydrogel viscoelasticity and recent studies demonstrating an impact of viscoelasticity on cells.
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3D Cell Culture Systems: Advantages and Applications
Journal of Cellular Physiology, 2014Cell cultures are important material of study for the variety of advantages that they offer. Both established continuous cell lines and primary cell cultures continue to be invaluable for basic research and for direct applications. Technological advancements are necessary to address emerging complex challenges and the way cells are cultured in vitro is
Maddaly, Ravi +4 more
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3D Cell Culture: An Introduction
20173D cell culture is an invaluable tool in developmental, cell, and cancer biology. By mimicking crucial features of in vivo environment, including cell-cell and cell-extracellular matrix interactions, 3D cell culture enables proper structural architecture and differentiated function of normal tissues or tumors in vitro.
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3D-Cultured Cell Image Acquisition
2016Acquiring high-content images of 3D-cultured cells for analyzing multiple cellular events is a daunting task, requiring an automated fluorescent microscope and high-throughput image analysis software. High throughput is an important feature for high-content imaging (HCI) devices to enable rapid image acquisition.
Pranav Joshi +3 more
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Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds
In vitro cell cultures are a very useful tool for the validation of biomaterial cytocompatibility, especially for bone tissue engineering scaffolds and bone implants. In this chapter, a protocol for a static three-dimensional osteoblast cell culture on titanium scaffolds and subsequent analysis of osteogenic capacity is presented.Carolina Oliver-Urrutia +5 more
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