Results 11 to 20 of about 629,047 (217)
Cells, 2020 Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well
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The Importance of Proper Oxygenation in 3D Culture [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Cell culture typically employs inexpensive, disposable plasticware, and standard humidified CO2/room air incubators (5% CO2, ∼20% oxygen). These methods have historically proven adequate for the maintenance of viability, function, and proliferation of many cell types, but with broad variation in culture practices.
Hubert M. Tse +4 more
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Deterministic culturing of single cells in 3D [PDF]
Scientific Reports, 2020 AbstractModels using 3D cell culture techniques are increasingly accepted as the most biofidelic in vitro representations of tissues for research. These models are generated using biomatrices and bulk populations of cells derived from tissues or cell lines. We present an alternate method to culture individually selected cells in relative isolation from
Rohil Jain +4 more
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The challenge of ovarian tissue culture: 2D versus 3D culture [PDF]
Journal of Ovarian Research, 2021 Abstract Background Cryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine function. To increase the longevity of the transplant and decrease the risk of reimplantation of neoplastic cells, several studies have been carried out with culture of
Ana Sofia Pais +6 more
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3D Cell Culture in Alginate Hydrogels [PDF]
Microarrays, 2015 This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of
Andersen, Therese +2 more
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Characterization of in vitro 3D cultures [PDF]
APMIS, 2021 SummaryOver the past decade, 3D culture models of human and animal cells have found their way into tissue differentiation, drug development, personalized medicine and tumour behaviour studies. Embryoid bodies (EBs) are in vitro 3D cultures established from murine pluripotential stem cells, whereas tumoroids are patient‐derived in vitro 3D cultures ...
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3D Culture of MSCs for Clinical Application. [PDF]
Bioengineering (Basel)Mesenchymal stem cells (MSCs) play an important role in regenerative medicine and drug discovery due to their multipotential differentiation capabilities and immunomodulatory effects. Compared with traditional 2D cultures of MSCs, 3D cultures of MSCs have emerged as an effective approach to enhance cell viability, proliferation, and functionality, and ...
Gao Q +10 more
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Alzheimer's in 3D culture: Challenges and perspectives [PDF]
BioEssays, 2015 Alzheimer's disease (AD) is the most common cause of dementia, and there is currently no cure. The “β‐amyloid cascade hypothesis” of AD is the basis of current understanding of AD pathogenesis and drug discovery. However, no AD models have fully validated this hypothesis.
Carla, D'Avanzo +5 more
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Recent Advances in 3D Cultures
International Journal of Molecular SciencesMethods and protocols for creating complex 3D cell culture systems have been rapidly advancing in the past decade from the perspective of biomaterials [...]
Picchio, Vittorio +2 more
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Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
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