Results 11 to 20 of about 6,043,899 (313)
Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders.
Joni H. Ylostalo
doaj +3 more sources
The challenge of ovarian tissue culture: 2D versus 3D culture [PDF]
Background Cryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine function.
Ana Sofia Pais +6 more
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The Importance of Proper Oxygenation in 3D Culture [PDF]
Cell culture typically employs inexpensive, disposable plasticware, and standard humidified CO2/room air incubators (5% CO2, ∼20% oxygen). These methods have historically proven adequate for the maintenance of viability, function, and proliferation of many cell types, but with broad variation in culture practices.
Hubert M. Tse +4 more
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Implementation of an in vitro exposure system for 28 GHz
The objective of this study was to implement an in vitro exposure system for 28 GHz to investigate the biological effects of fifth‐generation (5G) communication. A signal source of 28 GHz for 5G millimeter‐wave (MMW) deployment was developed, followed by
Young Seung Lee +7 more
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Three-dimensional model of glioblastoma by co-culturing tumor stem cells with human brain organoids
Emerging three-dimensional (3D) cultures of glioblastoma are becoming powerful models to study glioblastoma stem cell behavior and the impact of cell–cell and cell–microenvironment interactions on tumor growth and invasion.
Roberta Azzarelli +3 more
doaj +1 more source
Characterization of in vitro 3D cultures [PDF]
SummaryOver the past decade, 3D culture models of human and animal cells have found their way into tissue differentiation, drug development, personalized medicine and tumour behaviour studies. Embryoid bodies (EBs) are in vitro 3D cultures established from murine pluripotential stem cells, whereas tumoroids are patient‐derived in vitro 3D cultures ...
openaire +2 more sources
Vertical cell-cell contact is an important difference between 2D- and 3D-culture models. We hypothesized that this difference in cell-cell contacts is critical in maintaining hepatocyte functions.
Ryo SUDO +3 more
doaj +1 more source
Deterministic culturing of single cells in 3D [PDF]
AbstractModels using 3D cell culture techniques are increasingly accepted as the most biofidelic in vitro representations of tissues for research. These models are generated using biomatrices and bulk populations of cells derived from tissues or cell lines. We present an alternate method to culture individually selected cells in relative isolation from
Rohil Jain +4 more
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Vascularization in 3D Cell Culture
The two-dimensional (2D) monoculture does not recapitulate the three-dimensional (3D) environment of in vivo vasculature. Endothelial cell (ECs) spheroids have been introduced as a 3D in vitro model exhibiting angiogenic responses and sprouting behavior ...
Bagli, E. +9 more
core +1 more source
3D Cell Culture in Alginate Hydrogels [PDF]
This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of
Andersen, Therese +2 more
openaire +2 more sources

