Results 21 to 30 of about 5,851,651 (312)
Hepato(Geno)Toxicity Assessment of Nanoparticles in a HepG2 Liver Spheroid Model
Nanomaterials, 2020 (1) In compliance with the 3Rs policy to reduce, refine and replace animal experiments, the development of advanced in vitro models is needed for nanotoxicity assessment. Cells cultivated in 3D resemble organ structures better than 2D cultures.
Elisabeth Elje +7 more
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Journal of Biomechanical Science and Engineering, 2009 The purpose of this study is to test a hypothesis that involvement of the low-magnitude, high frequency strain components in the walking-induced bone strain is essential to elicit the osteogenic response of osteoblasts to the bone strain.
Shigeo M. TANAKA, Hui B. SUN
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Journal of Biomechanical Science and Engineering, 2013 There is a demand for three-dimensional (3D) angiogenesis model including endothelial cells (ECs) and mesenchymal stem cells (MSCs), which are known to differentiate into pericytes, to construct stabilized and matured microvascular networks in vitro ...
Kyoko YAMAMOTO +8 more
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Intracellular Ca2+ Responses of 3D-Cultured Osteoblasts to Dynamic Loading
Journal of Biomechanical Science and Engineering, 2012 In bone cells, intracellular Ca2+ (iCa2+) functions as a second messenger in the mechanotransduction pathway. Its responses to mechanical stimulation can be observed microscopically on a rigid flat surface with a Ca2+ fluorescent indicator, generally ...
Shigeo M. TANAKA
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Implementation of an in vitro exposure system for 28 GHz
ETRI Journal, 2020 The objective of this study was to implement an in vitro exposure system for 28 GHz to investigate the biological effects of fifth‐generation (5G) communication. A signal source of 28 GHz for 5G millimeter‐wave (MMW) deployment was developed, followed by
Young Seung Lee +7 more
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Deterministic culturing of single cells in 3D [PDF]
Scientific Reports, 2020 AbstractModels using 3D cell culture techniques are increasingly accepted as the most biofidelic in vitro representations of tissues for research. These models are generated using biomatrices and bulk populations of cells derived from tissues or cell lines. We present an alternate method to culture individually selected cells in relative isolation from
Rohil Jain +4 more
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Three-dimensional model of glioblastoma by co-culturing tumor stem cells with human brain organoids
Biology Open, 2021 Emerging three-dimensional (3D) cultures of glioblastoma are becoming powerful models to study glioblastoma stem cell behavior and the impact of cell–cell and cell–microenvironment interactions on tumor growth and invasion.
Roberta Azzarelli +3 more
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Characterization of in vitro 3D cultures [PDF]
APMIS, 2021 SummaryOver the past decade, 3D culture models of human and animal cells have found their way into tissue differentiation, drug development, personalized medicine and tumour behaviour studies. Embryoid bodies (EBs) are in vitro 3D cultures established from murine pluripotential stem cells, whereas tumoroids are patient‐derived in vitro 3D cultures ...
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Journal of Biomechanical Science and Engineering, 2008 Vertical cell-cell contact is an important difference between 2D- and 3D-culture models. We hypothesized that this difference in cell-cell contacts is critical in maintaining hepatocyte functions.
Ryo SUDO +3 more
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3D Cell Culture in Alginate Hydrogels [PDF]
Microarrays, 2015 This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of
Andersen, Therese +2 more
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