Results 71 to 80 of about 668,339 (308)
Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion) [PDF]
, 2007 The aim of this study was to develop and validate a simple and compact bioreactor system for perfusion cell seeding and culture through 3-dimensional porous scaffolds.
Früh, Jennifer-Annika +5 more
core
FEBS Letters, EarlyView.Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai +4 more
wiley +1 more source
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2016 This review discusses historical milestones, recent developments and challenges in the area of 3D culture models with cardiovascular cell types. Expectations in this area have been raised in recent years, but more relevant in vitro research, more accurate drug testing results, reliable disease models and insights leading to bioartificial organs are ...
openaire +3 more sources
Differentiation of stem cell-derived pancreatic progenitors into insulin-secreting islet clusters in a multiwell-based static 3D culture system [PDF]
, 2023 Orbital shaker-based suspension culture systems have been in widespread use for differentiating human pluripotent stem cell (hPSC)-derived pancreatic progenitors toward islet-like clusters during endocrine induction stages.
Zhan, Lisa +11 more
core +1 more source
Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes
FEBS Letters, EarlyView.Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez +2 more
wiley +1 more source
Stem Cell Research, 2021 Background: Angiogenesis is an essential step in tissue engineering. MSC exosomes play an important role in angiogenesis. Functional biomolecules in exosomes vested by the culture microenvironment can be transferred to recipient cells and affects their ...
Wenling Gao +7 more
doaj +1 more source
Bioinspired Hydrogels for 3D Organoid Culture
CHIMIA, 2019 Abstract: Organoids have stepped into the limelight as unique in vitro systems for modeling organ development, function and disease. This review provides a perspective on how chemically defined, bio-inspired hydrogels could be used for replacing ill-defined matrices derived from the native extracellular matrix (ECM) that are used for ...
Delphine Blondel, Matthias P. Lutolf
openaire +4 more sources
3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications [PDF]
, 2019 Three-dimensional (3D) cell culture is often mentioned in the context of regenerative medicine, for example, for the replacement of ischemic myocardium with tissue-engineered muscle constructs.
Zuppinger, Christian
core +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article [PDF]
Cell JournalDamaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, anddysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment.Two dimensional (2D)
Hengameh Dortaj +6 more
doaj +1 more source