Results 201 to 210 of about 44,474 (235)
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Identification of 5-Methylcytosine in Complex Genomes
Methods, 1999Cytosine methylation is attracting new attention for regulatory roles in gene expression and there is an increasing interest in detecting, at a single-base resolution, any 5-methylcytosine in genes from complex genomes. Differential base modification by chemicals followed by PCR-based genomic sequencing procedures can provide the resolution ...
H, Thomassin, E J, Oakeley, T, Grange
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Photochemical Deamination and Demethylation of 5-Methylcytosine
Chemical Research in Toxicology, 1996Cytosine methylation is believed to play a pivotal role in eucaryotic cellular development as well as in viral latency. We have been investigating chemical mechanisms for the perturbation of methylation patterns, including the effects of ultraviolet radiation. We observed that, upon exposure to UV light, 5-methylcytosine (5mC) was converted to thymine,
E, Privat, L C, Sowers
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Organization of 5-methylcytosine in chromosomal DNA
Biochemistry, 1978The 5-methylcytosine residues of L-cells have been labeled with [methyl-3H]-L-methionine and their chromatin localization studied using deoxyribonucleases. The kinetics of micrococcal nuclease digestion showed that the methylated cytosine residues are concentrated within regions resistant to nuclease digestion and preferentially missing from those ...
A, Solage, H, Cedar
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The repair of 5-methylcytosine deamination damage
1993The fact that sites of cytosine methylation in DNA are mutagenic hotspots, and that the genome of higher eukaryotes is thus depleted of the modified sequence CpG is common knowledge — certainly in the field of DNA methylation. The reason why 5-methylcytosine is an endogenous mutagen would also appear to be clear: 5-methylcytosine deaminates to thymine,
K, Wiebauer +3 more
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5-Methylcytosine localised in mammalian constitutive heterochromatin
Nature, 1974DEOXY-5-METHYLCYTIDYLIC acid (5-MeC) is an almost universal, although minor component of DNA in plants and animals. It makes up 4–7% of the bases in plants1, no more than 1.5% of the bases in the mouse2 and even less in the human3. 5-MeC itself is not incorporated directly into DNA.
Miller, O J +3 more
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5-Methylcytosine and Its Derivatives
2014Epigenetics has undergone an explosion in the past decade. DNA methylation, consisting of the addition of a methyl group at the fifth position of cytosine (5-methylcytosine, 5-mC) in a CpG dinucleotide, is a well-recognized epigenetic mark with important functions in cellular development and pathogenesis.
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Direct Labeling of 5-Methylcytosine and Its Applications
Journal of the American Chemical Society, 2007Cytosine methylation is one of the most important epigenetic events, and much effort has been directed to develop a simple reaction for methylcytosine detection. In this paper, we describe the design of tag-attachable ligands for direct methylcytosine labeling and their application to fluorescent and electrochemical assays.
Kazuo, Tanaka +3 more
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RNA 5-Methylcytosine Analysis by Bisulfite Sequencing
2015Cells have developed molecular machineries, which can chemically modify DNA and RNA nucleosides. One particular and chemically simple modification, (cytosine-5) methylation (m(5)C), has been detected both in RNA and DNA suggesting universal use of m(5)C for the function of these nucleotide polymers.
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The 5‐methylcytosine content of DNA: Tissue specificity
Journal of Cellular Physiology, 1971AbstractUsing a new technique for the determination of the extent of DNA methylation by comparing the incorporation of radioactivity from deoxycytidine‐2‐C14 into DNA cytosine and 5‐methylcytosine (5MC), the 5MC content of DNA was found to be a tissue specific property in two systems: (1) mouse tissue culture cell lines, in which the level of 5MC ...
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Sequencing 5-methylcytosine residues by the bisulphite method
DNA Sequence, 1996Measuring patterns of cytosine methylation in genomic DNA is most efficiently accomplished by use of the bisulphite method. This method depends on the large difference in reactivity of cytosines relative to 5-Methyl cytosines in genomic DNA to bisulphite.
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