Results 1 to 10 of about 16,942 (208)

Cell identity and 5-hydroxymethylcytosine. [PDF]

Epigenetics Chromatin
Abstract Epigenetic factors underlie cellular identity through the regulation of transcriptional networks that establish a cell’s phenotype and function. Cell conversions are directed by transcription factor binding at target DNA which induce changes to identity-specific gene regulatory programs. The degree of cell plasticity is determined by
Honig F, Murrell A.
europepmc   +3 more sources

Formation and Abundance of 5‐Hydroxymethylcytosine in RNA [PDF]

ChemBioChem, 2015
AbstractRNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope‐tracing methodology to demonstrate that the ribonucleoside 5‐methylcytidine (m5C) is subject to oxidative processing in mammals ...
Sabrina M Huber, Pieter van Delft, Martin Bachman   +2 more
exaly   +7 more sources

5-Hydroxymethylcytosine Loss in Conjunctival Melanoma [PDF]

Dermatopathology, 2021
Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in conjunctival melanoma.
Stahl, A., Riggi, N., Nardou, K., Nicolas, M., Kaya, G., Moulin, A.   +5 more
openaire   +6 more sources

5-Hydroxymethylcytosine (5-hmC) Specific Enrichment [PDF]

BIO-PROTOCOL, 2012
5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via next ...
Keith Szulwach, Chun-Xiao Song, Chuan He, Peng Jin   +3 more
openaire   +4 more sources

CpG islands and 5-hydroxymethylcytosine [PDF]

Nature Reviews Cancer, 2012
exaly   +2 more sources

5-hydroxymethylcytosine [PDF]

Cancer Biology & Therapy, 2013
DNA methylation at the 5 position of cytosine (5-mC) has emerged as a key epigenetic marker that plays essential roles in various biological and pathological processes. 5-mC can be converted to 5-hydroxymethylcytosine (5-hmC) by the ten-eleven translocation (TET) family proteins, which is now widely recognized as the "sixth base" in the mammalian ...
Chao, Ye, Lan, Li
openaire   +2 more sources

Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine [PDF]

Nature Protocols, 2012
A complete understanding of the potential function of 5-hydroxymethylcytosine (5-hmC), a DNA cytosine modification in mammalian cells, requires an accurate single-base resolution sequencing method. Here we describe a modified bisulfite-sequencing method, Tet-assisted bisulfite sequencing (TAB-seq), which can identify 5-hmC at single-base resolution, as
Miao, Yu, Gary C, Hon, Keith E, Szulwach, Chun-Xiao, Song, Peng, Jin, Bing, Ren, Chuan, He   +6 more
openaire   +2 more sources

The 5-Hydroxymethylcytosine Landscape of Prostate Cancer

Cancer Research, 2022
Abstract Analysis of DNA methylation is a valuable tool to understand disease progression and is increasingly being used to create diagnostic and prognostic clinical biomarkers. While conversion of cytosine to 5-methylcytosine (5mC) commonly results in transcriptional repression, further conversion ...
Martin Sjöström, Shuang G. Zhao, Samuel Levy, Meng Zhang, Yuhong Ning, Raunak Shrestha, Arian Lundberg, Cameron Herberts, Adam Foye, Rahul Aggarwal, Junjie T. Hua, Haolong Li, Anna Bergamaschi, Corinne Maurice-Dror, Ashutosh Maheshwari, Sujun Chen, Sarah W.S. Ng, Wenbin Ye, Jessica Petricca, Michael Fraser, Lisa Chesner, Marc D. Perry, Thaidy Moreno-Rodriguez, William S. Chen, Joshi J. Alumkal, Jonathan Chou, Alicia K. Morgans, Tomasz M. Beer, George V. Thomas, Martin Gleave, Paul Lloyd, Tierney Phillips, Erin McCarthy, Michael C. Haffner, Amina Zoubeidi, Matti Annala, Robert E. Reiter, Matthew B. Rettig, Owen N. Witte, Lawrence Fong, Rohit Bose, Franklin W. Huang, Jianhua Luo, Anders Bjartell, Joshua M. Lang, Nupam P. Mahajan, Primo N. Lara, Christopher P. Evans, Phuoc T. Tran, Edwin M. Posadas, Chuan He, Xiao-Long Cui, Jiaoti Huang, Wilbert Zwart, Luke A. Gilbert, Christopher A. Maher, Paul C. Boutros, Kim N. Chi, Alan Ashworth, Eric J. Small, Housheng H. He, Alexander W. Wyatt, David A. Quigley, Felix Y. Feng   +63 more
openaire   +6 more sources

Alterations of 5-Hydroxymethylcytosine in Human Cancers [PDF]

Cancers, 2013
Prior to 2009, 5-methylcytosine (5-mC) was thought to be the only biologically significant cytosine modification in mammalian DNA. With the discovery of the TET enzymes, which convert 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC), however, intense interest has emerged in determining the biological function of 5-hmC.
Mariani, Christopher J., Madzo, Jozef, Moen, Erika L., Yesilkanal, Ali, Godley, Lucy A.   +4 more
openaire   +2 more sources

Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis [PDF]

Nature Communications, 2013
Little is known about how patterns of DNA methylation change during mammalian spermatogenesis. 5 hmC has been recognized as a stable intermediate of DNA demethylation with potential regulatory functions in the mammalian genome. However, its global pattern in germ cells has yet to be addressed.
Haiyun, Gan, Lu, Wen, Shangying, Liao, Xiwen, Lin, Tingting, Ma, Jun, Liu, Chun-Xiao, Song, Min, Wang, Chuan, He, Chunsheng, Han, Fuchou, Tang   +10 more
openaire   +2 more sources