Bio-Protocol, 2022 Nucleic acids in living organisms are more complex than the simple combinations of the four canonical nucleotides. Recent advances in biomedical research have led to the discovery of numerous naturally occurring nucleotide modifications and enzymes ...
Weiwei Yang +9 more
doaj +1 more source
Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease
npj Parkinson's Disease, 2017 5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains.
Reinhard Stöger +3 more
doaj +1 more source
The role of 5-hydroxymethylcytosine in human cancer [PDF]
Cell and Tissue Research, 2014 The patterns of DNA methylation in human cancer cells are highly abnormal and often involve the acquisition of DNA hypermethylation at hundreds or thousands of CpG islands that are usually unmethylated in normal tissues. The recent discovery of 5-hydroxymethylcytosine (5hmC) as an enzymatic oxidation product of 5-methylcytosine (5mC) has led to models ...
Gerd P, Pfeifer +3 more
openaire +2 more sources
Medium throughput bisulfite sequencing for accurate detection of 5-methylcytosine and 5-hydroxymethylcytosine [PDF]
, 2017 Background Epigenetic modifications of DNA, such as 5-methylcytosine and 5-hydroxymethycytosine, play important roles in development and disease.
Naguib Mechawar (143665) +36 more
core +1 more source
Quantitation and mapping of the epigenetic marker 5‐hydroxymethylcytosine [PDF]
BioEssays, 2017 We here review primary methods used in quantifying and mapping 5‐hydroxymethylcytosine (5hmC), including global quantification, restriction enzyme‐based detection, and methods involving DNA‐enrichment strategies and the genome‐wide sequencing of 5hmC.
Ying Qing +6 more
openaire +2 more sources
Intrinsic and Extrinsic Connections of Tet3 Dioxygenase with CXXC Zinc Finger Modules. [PDF]
, 2013 Tet proteins are emerging as major epigenetic modulators of cell fate and plasticity. However, little is known about how Tet proteins are targeted to selected genomic loci in distinct biological contexts.
Christine S. Schmidt +21 more
core +1 more source
Turnover and function of DNA methylation at transcription factor binding sites [PDF]
, 2014 Cell type identity is largely determined by regulatory networks consistent of various transcription factors. Transcription factor activity requires interaction with DNA and thus critically depends on the accessibility of binding motifs.
Feldmann, Angelika
core +1 more source
Supplementary Figure from The 5-Hydroxymethylcytosine Landscape of Prostate Cancer
, 2022 Supplementary Figure from The 5-Hydroxymethylcytosine Landscape of Prostate ...
Matti Annala (219688) +63 more
core +1 more source
Tissue-specific 5-hydroxymethylcytosine landscape of the human genome
Nature Communications, 2021 Charting the landscape of 5hmC in human tissues is fundamental to understanding its regulatory functions. Here, we systematically profiled the whole-genome 5hmC landscape at single-base resolution for 19 types of human tissues and found 5hmC shows tissue-
Bo He +11 more
doaj +1 more source
Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during pronuclear development in equine zygotes produced by ICSI [PDF]
, 2017 Background: Global epigenetic reprogramming is considered to be essential during embryo development to establish totipotency. In the classic model first described in the mouse, the genome-wide DNA demethylation is asymmetric between the paternal and the ...
Katrien Smits +7 more
core +1 more source