Results 11 to 20 of about 8,428,479 (200)
Recognition of 5-hydroxymethylcytosine by the Uhrf1 SRA domain. [PDF]
PLoS ONE, 2011 Recent discovery of 5-hydroxymethylcytosine (5hmC) in genomic DNA raises the question how this sixth base is recognized by cellular proteins. In contrast to the methyl-CpG binding domain (MBD) of MeCP2, we found that the SRA domain of Uhrf1, an essential
Carina Frauer +6 more
doaj +6 more sources
The 5-Hydroxymethylcytosine Landscape of Prostate Cancer
Cancer Research, 2022 Abstract Analysis of DNA methylation is a valuable tool to understand disease progression and is increasingly being used to create diagnostic and prognostic clinical biomarkers. While conversion of cytosine to 5-methylcytosine (5mC) commonly results in transcriptional repression, further conversion ...
Martin Sjöström +63 more
core +12 more sources
5-Hydroxymethylcytosine Profiling as an Indicator of Cellular State [PDF]
Epigenomics, 2013 DNA methylation is widely studied in the context of cancer. However, the rediscovery of 5-hydroxymethylation of DNA adds a new layer of complexity to understanding the epigenetic basis of development and disease, including carcinogenesis. There have been significant advances in techniques for the detection of 5-hydroxymethylcytosine and, with this ...
Laird +4 more
openaire +7 more sources
Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition
Nature Communications, 2017 Transcription activator-like effector proteins recognise specific DNA sequences via tandem repeats. Here the authors demonstrate TALEs can recognise the methylated bases 5mC and 5hmC, enabling them to detect epigenetic modifications.
Yuan Zhang +7 more
doaj +3 more sources
The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing. [PDF]
PLoS ONE, 2010 We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons.
Yun Huang +5 more
doaj +6 more sources
5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote.
PLoS ONE, 2012 BackgroundIn the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the ...
Juliette Salvaing +5 more
doaj +2 more sources
5-Hydroxymethylcytosine (5-hmC) Specific Enrichment [PDF]
Bio-Protocol, 2012 5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a ...
Keith Szulwach +3 more
doaj +3 more sources
5-Methylcytosine and 5-Hydroxymethylcytosine Signatures Underlying Pediatric Cancers
Epigenomes, 2019 In addition to the genetic variations, recent evidence has shown that DNA methylation of both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) underlies the pathogenesis of pediatric cancer. Given the high mortality rate, there is an urgent need
Shalu Jhanwar, Ajinkya Deogade
doaj +2 more sources
Nature Communications, 2016 Glioblastomas have distorted epigenomes. Here, the authors compare the genome-wide profiles of 5-methylcytosine and 5- hydroxymethylcytosine in glioblastoma and prefrontal cortex tissue reporting a correlation between these profiles and patients ...
Kevin C. Johnson +5 more
doaj +2 more sources
The emerging role of 5-hydroxymethylcytosine in neurodegenerative diseases [PDF]
Frontiers in Neuroscience, 2014 DNA methylation primarily occurs within human cells as a 5-methylcytosine (5mC) modification of the cytosine bases in CpG dinucleotides. 5mC has proven to be an important epigenetic mark that is involved in the control of gene transcription for processes
Sahar eAl-Mahdawi +5 more
doaj +4 more sources