Results 11 to 20 of about 9,742 (128)
eLife, 2021 Although high levels of 5-hydroxymethylcytosine (5hmC) accumulate in mammalian neurons, our knowledge of its roles in terminal differentiation or as an intermediate in active DNA demethylation is incomplete.
Elitsa Stoyanova +3 more
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5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
Bio-Protocol, 2012 5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a ...
Keith Szulwach +3 more
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Genome-wide characterization of cytosine-specific 5-hydroxymethylation in normal breast tissue
Epigenetics, 2020 Despite recent evidence that 5-hydroxymethylcytosine (5hmC) possesses roles in gene regulation distinct from 5-methylcytosine (5mC), relatively little is known regarding the functions of 5hmC in mammalian tissues.
Owen M. Wilkins +5 more
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Cell‐free DNA 5‐hydroxymethylcytosine as a marker for common cancer detection
Clinical and Translational Discovery, 2022 Background Early cancer detection can dramatically improve clinical outcomes and greatly reduce economic burden of patients. Plasma cell–free DNA (cfDNA) 5‐hydroxymethylcytosine (5hmC) is an emerging epigenetic marker for cancer diagnosis.
Jianming Shao +3 more
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Integrated single-cell sequencing of 5-hydroxymethylcytosine and genomic DNA using scH&G-seq
STAR Protocols, 2021 Summary: The asymmetric distribution of 5-hydroxymethylcytosine (5hmC) between two DNA strands of a chromosome enables endogenous reconstruction of cellular lineages at an individual-cell-division resolution. Further, when integrated with data on genomic
Alex Chialastri +2 more
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The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing. [PDF]
PLoS ONE, 2010 We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons.
Yun Huang +5 more
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Bio-Protocol, 2022 Nucleic acids in living organisms are more complex than the simple combinations of the four canonical nucleotides. Recent advances in biomedical research have led to the discovery of numerous naturally occurring nucleotide modifications and enzymes ...
Weiwei Yang +9 more
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Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease
npj Parkinson's Disease, 2017 5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains.
Reinhard Stöger +3 more
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5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote.
PLoS ONE, 2012 BackgroundIn the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the ...
Juliette Salvaing +5 more
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5-Methylcytosine and 5-Hydroxymethylcytosine Signatures Underlying Pediatric Cancers
Epigenomes, 2019 In addition to the genetic variations, recent evidence has shown that DNA methylation of both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) underlies the pathogenesis of pediatric cancer. Given the high mortality rate, there is an urgent need
Shalu Jhanwar, Ajinkya Deogade
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